Rapid Fractionation of Grape Seed Proanthocyanidins

Saucier, Cédric; Mirabel, Marie; Daviaud, Freddy; Longieras, and Antoine; Glories, Yves

doi:10.1021/jf010784f

Cited by 82 publications

(64 citation statements)

References 22 publications

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“…All patterns were evaluated by HPLC HP 1100, Lispher RP18 (5 mM) column equipped with a UV detector and a quaternary pump system, with a fl ow rate of 1.0 mL / min and an injection volume of 50 μL, and results were expressed in μg / mL. The quantifi cation of catechin was performed as described by Saucier et al (2001). Solvent A contained water with 5% acetic acid; solvent B contained methanol with 5% acetic acid; isocratic elution 90% A and 10% solvent B, total analysis time, 45 min, detection at 280 nm (Saucier et al 2001).…”

Section: Phenolic Compoundsmentioning

confidence: 99%

“…The quantifi cation of catechin was performed as described by Saucier et al (2001). Solvent A contained water with 5% acetic acid; solvent B contained methanol with 5% acetic acid; isocratic elution 90% A and 10% solvent B, total analysis time, 45 min, detection at 280 nm (Saucier et al 2001). For quantifi cation of rutin, quercetin, kaempferol and naringin, solvent A contained methanol with 2% acetic acid and solvent B Milli-Q water with 2% acetic acid, total analysis time, 60 min, detection at 350 nm.…”

Section: Phenolic Compoundsmentioning

confidence: 99%

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Evaluation of antioxidant activity of grapevine leaves extracts (Vitis labrusca) in liver of Wistar rats

Schaffer

Wohlenberg

Medeiros

et al. 2016

An. Acad. Bras. Ciênc.

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The aim of this study was to evaluate the hepatoprotection of organic and conventional grapevine leaves extract (Vitis labrusca). The total polyphenol content and the isolate polyphenols by HPLC were evaluate. The animals received intraperitoneal injections of saline or extracts (conventional or organic -30 mg/kg) for 14 days. On day 15, the rats received carbon tetrachloride (CCl 4 ) or mineral oil (i.p.). After 4h, the animals were euthanized. The analysis of the liver enzymes activity (AST, ALT, GGT) was performed using serum, obtained by blood and the levels of lipid peroxidation (TBARS), protein oxidation (carbonyl), and the activity of antioxidant enzymes superoxide dismutase and catalase were analyzed in the liver. The results showed that the organic extract is richer in polyphenol and resveratrol than the conventional one. Both extracts prevent lipid peroxidation and protein oxidation generated by CCl 4 . Moreover, the extracts demonstrated ability to modulate the activity of SOD and CAT, as well as to establish a balance in the ratio of SOD/CAT. We also found that the CCl 4 increased the levels of AST and GGT, and that both extracts prevent this. These results indicate that grapevine leaves extracts, both, organic and conventional, can prevent liver disorders.

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Section: Phenolic Compoundsmentioning

confidence: 99%

Section: Phenolic Compoundsmentioning

confidence: 99%

Evaluation of antioxidant activity of grapevine leaves extracts (Vitis labrusca) in liver of Wistar rats

Schaffer

Wohlenberg

Medeiros

et al. 2016

An. Acad. Bras. Ciênc.

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show abstract

“…All patterns were evaluated using an HPLC HP 1100 with Lichrospher RP-18 (5 µm) column, equipped with a UV detector and a quaternary pump system, with a flow rate of 1.0 mL/min and an injection volume of 50 µL, and the results were expressed in µg/ml. The quantification of catechin was performed as described by Saucier et al (2001), where solvent A consisted of water with 5% acetic acid, and solvent B consisted of methanol with 5% acetic acid. Separation was by isocratic elution with 90% A and 10% solvent B; total analysis time was 45 min, and detection at 280 nm.…”

Section: Phenolicsmentioning

confidence: 99%

In vitro antioxidant capacity of tea of Echinodorus grandiforus, “leather hat,” in Wistar rat liver

Lunardi¹,

Wohlenberg²,

Medeiros³

et al. 2014

An. Acad. Bras. Ciênc.

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Oxidative stress has been considered as one of the factors responsible for hepatic diseases, which sometimes require new ways of treatment. The present study aimed to evaluate the in vitro antioxidant capacity of the tea of Echinodorus grandiforus ("leather hat" plant) in rat liver. Different preparations of tea were evaluated for phenolic composition, antioxidant activity by DPPH assay and ability to inhibit lipid peroxidation induced by copper sulfate. The antioxidant activity was assessed in liver tissue treated with sodium azide in the presence or absence of tea by assays for lipid peroxidation (TBARS), protein oxidation (carbonyl) and the antioxidant enzymes catalase (CAT) and superoxide dismutase (SOD). The results show that different preparations of tea are important sources of polyphenols and contain theobromine, catechin and vitexin. Furthermore, the results indicate that this tea exhibits an antioxidant activity by its ability to scavenge DPPH radical. Different preparations of tea prevented damage to lipids and proteins induced by sodium azide, as well as assisting in restoring CAT and SOD activities. Thus, it can be seen that E. grandiforus tea had antioxidant activity in serum and liver being able to prevent oxidative damages generated by sodium azide.

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“…Extraction of CTs was carried out as described by Geny et al (2003) and measurements were performed according to the method of Saucier et al (2001). HPLC analyses were carried out on a Spectra Physics system fitted with a UV visible detector driven by a PC1000 system.…”

Section: Quantification Of Pa-derived Compoundsmentioning

confidence: 99%

Characterization of a Grapevine R2R3-MYB Transcription Factor That Regulates the Phenylpropanoid Pathway

et al. 2005

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The ripening of grape (Vitis vinifera) berry is characterized by dramatic changes in gene expression, enzymatic activities, and metabolism that lead to the production of compounds essential for berry quality. The phenylpropanoid metabolic pathway is one of the components involved in these changes. In this study, we describe the cloning and functional characterization of VvMYB5a, a cDNA isolated from a grape L. cv Cabernet Sauvignon berry library. VvMYB5a encodes a protein belonging to a small subfamily of R2R3-MYB transcription factors. Expression studies in grapevine indicate that the VvMYB5a gene is mainly expressed during the early steps of berry development in skin, flesh, and seeds. Overexpression of VvMYB5a in tobacco (Nicotiana tabacum) affects the expression of structural genes controlling the synthesis of phenylpropanoid and impacts on the metabolism of anthocyanins, flavonols, tannins, and lignins. Overexpressing VvMYB5a induces a strong accumulation of several phenolic compounds, including keracyanin (cyanidin-3-rhamnoglucoside) and quercetin-3-rhamnoglucoside, which are the main anthocyanin and flavonol compounds in tobacco. In addition, VvMYB5a overexpression increases the biosynthesis of condensed tannins and alters lignin metabolism. These findings suggest that VvMYB5a may be involved in the control of different branches of the phenylpropanoid pathway in grapevine.

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Rapid Fractionation of Grape Seed Proanthocyanidins

Cited by 82 publications

References 22 publications

Evaluation of antioxidant activity of grapevine leaves extracts (Vitis labrusca) in liver of Wistar rats

Evaluation of antioxidant activity of grapevine leaves extracts (Vitis labrusca) in liver of Wistar rats

In vitro antioxidant capacity of tea of Echinodorus grandiforus, “leather hat,” in Wistar rat liver

Characterization of a Grapevine R2R3-MYB Transcription Factor That Regulates the Phenylpropanoid Pathway

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