Rapid green synthesis of silver nano particles from <i>Ziziphus mauritiana</i> and antibacterial activity against human pathogens

Abstract

A novel green source Ziziphus mauritiana fresh young leaves was opted to synthesize silver nanoparticles and analyze its antibacterial activity. The bioactive compounds present in the plant extracts reduced silver ions to NPs, indicated by change in color from red to dark brown. In this study, we have successfully synthesized nanoparticles using Z. mauritiana aqueous leaf extract as a reducing agent and the reaction process of synthesized nanoparticles was monitored by UV-Vis spectroscopy. The UV-Vis absorption peak showed maximum adsorption at 420 nm confirmed the silver nanoparticles synthesis. Further characterization was carried out by FTIR and the results recorded a downward shift of absorption the bands between 400 to 4000 cm-1 indicates the formation of silver nanoparticles. Finally, the present research was exploited to study the antibacterial activity of synthesized nanoparticles produced Z. mauritiana was studied using different pathogenic bacteria such as Salmonella sp., Proteus sp., Bacillus sp., Klebsiella pneumonia and E.coli from the well diffusion results, the synthesized silver nanoparticles displayed the best antibacterial property as compared to the antibiotic has been reported in this paper. To the best of our knowledge, this is the first report that the Z. mauritiana aqueous extract facilitate the synthesis of silver nanoparticles and also exhibits antibacterial activity.

Restoration of Antibacterial Activity of Inactive Antibiotics via Combined Treatment with AgNPs

“…Interpretations of Selvaraj et al (2019) are correlated with our findings . Similar results were also presented by Amutha and Sridhar …”

“…sativa are already reported. The availability of H + ions is considered responsible for the reduction of silver ions into silver NPs. − Visual detection of the formation of green synthesized nanoparticles with the metal precursor and the plant extract is also confirmed by many researchers. , …”

“… 44 − 46 Visual detection of the formation of green synthesized nanoparticles with the metal precursor and the plant extract is also confirmed by many researchers. 47 , 48 …”

Inhibition of biofilm, quorum‐sensing, and swarming motility in pathogenic bacteria by magnetite, manganese ferrite, and nickel ferrite nanoparticles

“…Various NPs of iron oxide have shown variable antibacterial activity against gram‐positive bacteria, including S. aureus and enterococcus, thereby confirming the results obtained in this study 69–71 . In the same light, many gram‐negative bacteria, including those reported in this study like P. aeruginosa and E. coli , have been shown to be susceptible to antibacterial effects of magnetic iron NPs 72–75 . The inhibition of fungal ( Candida ) species by magnetic iron oxide NPs reported in this study ascertains other findings that describe anticandidal activity that has been reported previously 76 .…”

“…[69][70][71] In the same light, many gram-negative bacteria, including those reported in this study like P. aeruginosa and E. coli, have been shown to be susceptible to antibacterial effects of magnetic iron NPs. [72][73][74][75] The inhibition of fungal (Candida) species by magnetic iron oxide NPs reported in this study ascertains other findings that describe anticandidal activity that has been reported previously. 76 It has been noted that microbes have less resistance to NPs and usually show bacteriostatic and bactericidal susceptibilities toward nanomaterials.…”