Rapid, highly efficient extraction and purification of membrane proteins using a microfluidic continuous-flow based aqueous two-phase system

Optimization and miniaturization of aqueous two phase systems for the purification of recombinant human immunodeficiency virus-like particles from a CHO cell supernatant

“…They are cost-effective since a large number of variables can be evaluated in parallel, reducing the sample/ reagent volumes required, and promote lab-scale process similar to the continuous large-scale processes. Such technique has been applied to the purification of biomolecules such as monoclonal antibodies [26], bovine serum albumin [27], membrane proteins from crude cell extract [28], and recombinant proteins from a cell lysate [29]. The main advantages of this design are (i) the possibility of adjusting the length and width of the separation channel in order to speed up the corresponding purification and separation process by decreasing diffusion times; (ii) the simple operation; (iii) the very low amount of required reagents, of the order of 1-100 lL;…”

A novel membrane based process to isolate recombinant human chemokine receptor CCR3 produced in Escherichia coli

“…Despite the critical significance of CCR3 in allergic diseases treatment, studies on its structure and function are relatively hampered for three main reasons: (a) natural CCR3 is at low abundance; (b) production of CCR3 in heterologous system is extremely difficult [4]; and (c) the isolation process of CCR3 using conventional techniques (e.g. chromatography and ultracentrifugation) takes more than 20 h and it is difficult to scale-up, resulting in less than 60% low-yield rate [5,6].…”

Thiolene and SIFEL-based microfluidic platforms for liquid–liquid extraction

“…Vigorous agitation of the phases is required to (1) shorten the distances that the chemicals need to traverse by diffusion to the aqueous-organic interface; (2) minimize the formation of the depletion and saturation layers on either side of the liquid-liquid interface; and (3) maximize the contact area. This need for agitation of the phases for efficient extraction necessitates the use of large volumes of aqueous and organic phases, which hampers the utility of LLE for applications involving small reagent volumes (<1 mL), such as sample preparation and/or chemical/ biochemical analysis of expensive, limitedly available chemicals [12–14], and extraction of molecules occurring in low concentrations ([15, 16]. …”