Rapid, highly sensitive detection of Gram-negative bacteria with lipopolysaccharide based disposable aptasensor

Zhang, Jian; Oueslati, Rania; Cheng, Cheng; Zhao, Ling; Chen, Jiangang; Almeida, Ricardo Augusto Monteiro de Barros

doi:10.1016/j.bios.2018.04.034

Cited by 58 publications

(34 citation statements)

References 45 publications

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“…The change of normalized capacitance per minute, i.e., dC/dt (%/min), directly reflects the S-protein adsorption level, by which the S-protein concentration in solution can be indicated. Using an IDME of micron scale, dC/dt is a competitive parameter to reflect tiny change at the electrode interface [30,37,43]. Therefore, ultra-trace S-protein detection can be expected.…”

Section: Methodsmentioning

confidence: 99%

Real-time Sensing of Trace Biomarkers from Viruses with a Microfluidic Immunosensor: A Case Study of SARS-CoV-2 Detection in Cold-chain Food

Qi¹,

Zhang

Cheng³

et al. 2020

Preprint

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Section: Methodsmentioning

confidence: 99%

Real-time Sensing of Trace Biomarkers from Viruses with a Microfluidic Immunosensor: A Case Study of SARS-CoV-2 Detection in Cold-chain Food

Qi¹,

Zhang

Cheng³

et al. 2020

Preprint

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“…The following subsection highlights two electrochemical biosensors for the detection of E. coli. The first highly sensitive electrochemical biosensor was recently developed by Zhang et al for the detection of gram-negative bacteria using commercially available microelectrodes functionalized with a liposaccharide (LPS) specific thiolated aptamer (K d = 12 nM, Kim et al, 2012), and E. coli as the model gram-negative bacteria to develop and optimize the biosensor (Zhang et al, 2018d). This assay could detect as few as 267 cells/mL in as little as 30 s, however detection in an environmental sample was not demonstrated.…”

Section: Escherichia Colimentioning

confidence: 99%

Aptamer-Based Biosensors for Environmental Monitoring

2020

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Due to their relative synthetic and chemical simplicity compared to antibodies, aptamers afford enhanced stability and functionality for the detection of environmental contaminants and for use in environmental monitoring. Furthermore, nucleic acid aptamers can be selected for toxic targets which may prove difficult for antibody development. Of particular relevance, aptamers have been selected and used to develop biosensors for environmental contaminants such as heavy metals, small-molecule agricultural toxins, and water-borne bacterial pathogens. This review will focus on recent aptamer-based developments for the detection of diverse environmental contaminants. Within this domain, aptamers have been combined with other technologies to develop biosensors with various signal outputs. The goal of much of this work is to develop cost-effective, user-friendly detection methods that can complement or replace traditional environmental monitoring strategies. This review will highlight recent examples in this area. Additionally, with innovative developments such as wearable devices, sentinel materials, and lab-on-a-chip designs, there exists significant potential for the development of multifunctional aptamer-based biosensors for environmental monitoring. Examples of these technologies will also be highlighted. Finally, a critical perspective on the field, and thoughts on future research directions will be offered.

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“…This type of biosensors requires only one recognition element, which leads to simplification of the pattern of analysis, reducing both the analysis time and expenditures for reagents. The latest generation of label-free biosensors provides real-time quantification of products of biomolecular reaction, which makes it possible to perform continuous data recording, allowing kinetic monitoring of parameters of the ligand-receptor interaction recognition process [24,32,[77][78][79][80].…”

Section: Label-free Biosensorsmentioning

confidence: 99%

“…Moreover, an important advantage of the use of label-free biosensors is that target analytes are detected in their natural form without labeling and chemical modification, and thus, they can be preserved for further analysis (Table 2). [ 22,26,39,80,86] In recent decades, numerous studies have been carried out to develop new types of receptors [29,63,87,88] and methods of recognition for label-free biosensors. They can generate signal immediately after binding to the recognition element and do not require additional interactions with the labels that provide signal.…”

Section: Label-free Biosensorsmentioning

confidence: 99%

“…Optical, electrochemical, electric (piezoelectric), microwave, or (micro) mechanical transducers are promising strategies for recognizing ligand-receptor interaction signals in label-free biosensors used to indicate biological agents or biomonitoring the environment [32,40,80,84]. Among the most powerful detection and analysis tools, widely used in biomedical research and in practical medicine, are biosensors with optical transducers, considered the basic tool for signal perception [3,7,75,89,90].…”

Section: Label-free Biosensorsmentioning

confidence: 99%

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Label-Free Biosensors for Laboratory-Based Diagnostics of Infections: Current Achievements and New Trends

et al. 2020

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Infections pose a serious global public health problem and are a major cause of premature mortality worldwide. One of the most challenging objectives faced by modern medicine is timely and accurate laboratory-based diagnostics of infectious diseases. Being a key factor of timely initiation and success of treatment, it may potentially provide reduction in incidence of a disease, as well as prevent outbreak and spread of dangerous epidemics. The traditional methods of laboratory-based diagnostics of infectious diseases are quite time- and labor-consuming, require expensive equipment and qualified personnel, which restricts their use in case of limited resources. Over the past six decades, diagnostic technologies based on lateral flow immunoassay (LFIA) have been and remain true alternatives to modern laboratory analyzers and have been successfully used to quickly detect molecular ligands in biosubstrates to diagnose many infectious diseases and septic conditions. These devices are considered as simplified formats of modern biosensors. Recent advances in the development of label-free biosensor technologies have made them promising diagnostic tools that combine rapid pathogen indication, simplicity, user-friendliness, operational efficiency, accuracy, and cost effectiveness, with a trend towards creation of portable platforms. These qualities exceed the generally accepted standards of microbiological and immunological diagnostics and open up a broad range of applications of these analytical systems in clinical practice immediately at the site of medical care (point-of-care concept, POC). A great variety of modern nanoarchitectonics of biosensors are based on the use of a broad range of analytical and constructive strategies and identification of various regulatory and functional molecular markers associated with infectious bacterial pathogens. Resolution of the existing biosensing issues will provide rapid development of diagnostic biotechnologies.

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Rapid, highly sensitive detection of Gram-negative bacteria with lipopolysaccharide based disposable aptasensor

Cited by 58 publications

References 45 publications

Real-time Sensing of Trace Biomarkers from Viruses with a Microfluidic Immunosensor: A Case Study of SARS-CoV-2 Detection in Cold-chain Food

Real-time Sensing of Trace Biomarkers from Viruses with a Microfluidic Immunosensor: A Case Study of SARS-CoV-2 Detection in Cold-chain Food

Aptamer-Based Biosensors for Environmental Monitoring

Label-Free Biosensors for Laboratory-Based Diagnostics of Infections: Current Achievements and New Trends

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