2014
DOI: 10.1021/jf4053258
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Rapid HPLC-MS Method for the Simultaneous Determination of Tea Catechins and Folates

Abstract: An effective and rapid HPLC-MS method for the simultaneous separation of the eight most abundant tea catechins, gallic acid, and caffeine was developed. These compounds were rapidly separated within 9 min by a linear gradient elution using a Zorbax SB-C18 packed with sub 2 μm particles. This methodology did not require preparative and semipreparative HPLC steps. In fact, diluted tea samples can be easily analyzed using HPLC-MS as described in this study. The use of mass spectrometry detection for quantificatio… Show more

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Cited by 33 publications
(22 citation statements)
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“…The catechin contents (ECG, EGCG, EC, and EGC) of the tea extracts were measured by high-performance liquid chromatography (HPLC) 30) . Concentrations of 1.25, 2.50, 5.00, 10.00, and 20.00 mg/mL of the AGW, TGY, and DHP extracts were prepared by diluting the tea extracts with distilled water.…”
Section: Catechin Measurementmentioning
confidence: 99%
“…The catechin contents (ECG, EGCG, EC, and EGC) of the tea extracts were measured by high-performance liquid chromatography (HPLC) 30) . Concentrations of 1.25, 2.50, 5.00, 10.00, and 20.00 mg/mL of the AGW, TGY, and DHP extracts were prepared by diluting the tea extracts with distilled water.…”
Section: Catechin Measurementmentioning
confidence: 99%
“…Unfortunately, analysis of FA is not an easy task because of its instability under acidic conditions and sensitivity to light and heat. Conventional analytical techniques employed for the FA quantification mainly include high performance liquid chromatography-mass spectrometry (HPLC-MS) (Monica, Alain, Elodie, & Laurent, 2014), LC-MS/MS (Vishnumohan, Arcot, & Pickford, 2011), bioassays, and fluorimetric methods (Hemmateenejad, Shakerizadehshirazi, & Samari, 2014), which could provide available and reliable test results. It is a pity that they all have the individual drawbacks, for instance, tedious sample pretreatment of HPLC, time-consuming detection of bioassays, difficult design of fluorimetric method.…”
Section: Introductionmentioning
confidence: 99%
“…Green tea also contains other polyphenols such as gallic acid, the main tea polyphenolic acid, and certain amounts of alkaloids such as caffeine. These compositions of green tea vary according to its variety, climate, horticultural conditions, and particularly technologies applied during the manufacturing process (Araya‐Farias, Gaudreau, Rozoy, & Bazinet, 2014). The main chemical changes of tea polyphenols during green tea processing are oxidation, hydrolysis, polymerization, and transformation.…”
Section: Introductionmentioning
confidence: 99%