“…First, a multislice, T 2 weighted imaging sequence (RARE) was used to provide location information on the cells or tumor with the following parameters: 55 TE/TR = 24/3000 ms, RARE factor = 8, NAV = 1, 15 axial slices with 1.5 mm thickness, matrix size = 128 × 128, 30 × 30 mm field of view (FOV). Total acquisition time was 48 s. Next, a single slice, T 2 -mapping Carr–Purcell–Meiboom–Gill sequence was optimized to detect the boundary of the lesions at the xenograft site with the following parameters: 56 TE = 8, 16, 24...512 ms (64 echoes), TR = 1000 ms, NAV = 2, 1.5 mm thickness, matrix size = 128 × 128, 30 × 30 mm FOV. Total acquisition time was 4 min 16 s. After imaging, the cell samples were collected and sonicated at 30% power for 30 s in ice, and the total protein content was measured using the Quick Start Bradford Protein Assay with bovine serum albumin as standard (Biorad, Hercules, CA).…”