Rapid Identification and Simultaneous Quantification of Aristolochic Acids by HPLC-DAD and Confirmations by MS in Aristolochia chilensis Using a Limited Biomass

Abstract

Six aristolochic acids were identified in the Chilean species Aristolochia chilensis using high-performance liquid chromatography coupled to a diode array detector (HPLC-DAD) and subsequent confirmation with mass spectrometry (MS). The fractions of each signal were collected and injected directly into an Orbitrap mass detector model Q Exactive Focus (Thermo Scientific). The acids extraction was done with 0.10–0.50 g of lyophilized and pulverized sample and concentrated in Soxhlet extraction equipment. The liquid-liquid separations and a subsequent solid phase extraction (SPE) C18 were performed using 100 µL of the extract that contains the aristolochic acids present in the Aristolochia chilensis plant. The HPLC conditions used a single mobile phase acetonitrile : water (1 : 1) acidified with 0.1% acetic acid and an isocratic elution to 1 mL·min−1. The column InertSustain C18 250 × 4.6 mm and 3 µm was used, the injection volume was 20 µL, and the time of run was reduced to 15 min. Calibration curves were constructed with r 2 being 0.9997. The quantification limit for AAI was 0.138 ± 0.010 µg/mL, and for AAII, it was 0.558 ± 0.042 µg/mL.

Pitfalls and promises of raw drug identification techniques in the ayurvedic industry: an overview

“…In addition, presence and quantification of toxic chemicals from plant species such as aristolochic acids in Aristolochia, norditerpenoid alkaloids in Delphinium spp, and identification of five hepatotoxic pyrrolizidine alkaloids in traditional Chinese medicinal herb, Herba senecionis var. scandentis, have been identified using HPLC (Araya et al 2018;Li et al 2008). HPLC is possible to hyphenate with other detectors such as UV, DAD, for herbal finger printing and NMR for metabolomic profiling (Kamboj and Saluja 2017).…”

Rapid Analysis of Aristolochic Acid Analogues in Traditional Chinese Patent Medicine by LC-MS/MS

“…At present, different methods have been reported for determination of AAs, including thin-layer chromatography (TLC), high performance liquid chromatography (HPLC), liquid chromatography-mass spectrometry (LC-MS), enzymelinked immunosorbent assay (ELISA), and capillary electrophoresis (CE) [9][10][11]. e HPLC and LC-MS methods are also widely used in characterizing the AA analogues [12][13][14][15][16][17][18]. HPLC is more suitable for those with higher content of AA analogues.…”

Development of ic-ELISA and an immunochromatographic strip assay for the detection of aristolochic acid Ⅰ

“…To ensure the safety and effectiveness of clinical drug use, strict testing must be carried out for medicinal plant use, including preparations containing AAs, and a standard for limited drug use must be established. At present, the detection of AA Ⅰmainly involves thin layer chromatography (Agrawal & Laddha, 2017;Liu, Bi, Chu, & Li, 2010), and high-performance liquid chromatography (Araya, Garcia, & Gonzalezteuber, 2018;Yamasaki et al, 2009;Yuan et al, 2008). The sensitivity of thin layer chromatography is low, whereas high-performance liquid chromatography has high sensitivity and is more accurate.…”