Rapid Identification of Antibacterial Compounds from Turkey Berry by HPLC-Based Metabolomics

Maser, Wahyu Haryati; Yuliana, Nancy Dewi; Andarwulan, Nuri

doi:10.1080/10826076.2015.1032419

Cited by 11 publications

(8 citation statements)

References 11 publications

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“…The ethanolic extract was fractionated using manual liquid-liquid sequential solvent extraction based on the method by Maser et al (2015). Ethanol extract were dissolved in distilled water (1:30 w/v) and partitioned gradually in separator funnel using n-hexane, chloroform, and ethyl acetate, respectively (Merck, 1:1 v/v ratio).…”

Section: Extraction and Fractionationmentioning

confidence: 99%

Antibacterial stability of Spondias pinnata (L. f.) Kurz leaves extract and its mechanism

Asnani¹,

Rahayu²,

Jenie³

et al. 2018

MJM

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Aims: This study was conducted to observe the stability of Spondias pinnata leaf (SPL) extract antibacterial at different pH, salt concentration and temperature, to examine its antibacterial effectivity on minced fish, and to determine its fraction's antibacterial mechanism. Methodology and results: The tested SPL ethanolic extract, n-hexane, chloroform, ethyl acetate and water fractions' antibacterial activity against Bacillus cereus and Vibrio parahaemolitycus. Its stability against pH, salt, and thermal variation was studied, as well as the mechanism and application in fish. Ethyl acetate fraction and water fraction showed the highest activity against B. cereus (MIC 0.62 mg/mL). Protein profile analysis using gel electrophoresis showed that B. cereus cells exposed with SPL ethyl acetate fraction and water fraction showed thinner protein bands as compared to control. Severe damage of the cells treated with 3 MIC was also observed under SEM. Antibacterial activity of SPL ethanolic against Bacillus cereus and Vibrio parahaemolitycus were stable against heat treatment (80-121 °C for 15 min) and NaCl treatment (0-10% w/v), whereas the inhibition zone respectively at pH 4 (10.31±0.25 and 8.09±0.97 mm) was higher than pH 7 (8.45±0.52 and 6.66±1.84 mm). Application of SPL ethanolic extract in fish broth showed higher antibacterial activity than in fish flesh, which gave bactericidal effect at 3 MIC. Conclusion, significance and impact study: Ethanol extract can be developed as a natural preservative in fish processing.

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Section: Extraction and Fractionationmentioning

confidence: 99%

Antibacterial stability of Spondias pinnata (L. f.) Kurz leaves extract and its mechanism

Asnani¹,

Rahayu²,

Jenie³

et al. 2018

MJM

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“…Analysis of specific bioactive chemical profiles used HPLC instrument (HPLC Agilent Technologies, Multi Wavelength Detektor (MWD) with C18 column (5 μm, 150 x 4.6 mm)). The chemical profile analysis of specific bioactive compounds by HPLC followed (Andarwulan et al, 2010) and (Maser et al, 2015) with few modifications. Samples (CE, FH, FC, FEA and FW) in 0.8 μg/mL concentration in methanol were filtered by regenerated membrane 0.45 µm.…”

Section: Extract/fraction Specific Bioactive Chemical Profile By Hplcmentioning

confidence: 99%

“…The spots of TLC from isolated results were identified by using UHPLC-MS/MS tandem mass spectrometer (UHPLC Vanquish Tandem Q Exactive Plus Orbitrap HRMS ThermoScientific, column Accucore phenyl hexyl, 100 x 2.1 mm, 2.6 µm). The principle of UHPLC-MS/MS refers to Yulianto et al (2017) and Maser et al (2015). Spots extract were diluted with 100 μL methanol and then filtrated by filter membrane 0.45 µm.…”

Section: Sample Identification With Bioactive Chemical Profile With Tmentioning

confidence: 99%

Identification of antioxidant bioactive compounds as potential functional food ingredient from kebar grass (Biophytum petersianum) by metabolomic approach

et al. 2022

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Kebar grass (Biophytum petersianum) is traditionally believed as a food ingredient to increase the health of the reproductive. The specific bioactive compounds with antioxidant effect in this plant have not been reported before. The objective of this research was to identify the specific bioactive compounds as a source of functional food ingredients of Kebar grass (KG) which have antioxidant activity by using a metabolomic approach. Samples chemical profile (extract and fraction) used HPLC, followed by metabolomic analysis by OPLS. Chosen samples were isolated by TLC and then identified by UHPLC-MS/MS. Specific bioactive compounds were identified from chromatogram with putative identification by MZmine referring to online databases. The metabolomic result obtained ethyl acetate fraction (FEA) as the sample that most contributed to antioxidant activity. FEA identification result showed ten specific bioactive compounds that are thought to contribute to antioxidant agents. Two of the compounds were identified as caffeic acid and cassiaoccidentalin A, while the other eight compounds have not been identified. The finding of cassiaoccidentalin A in KG is a novel finding and can be used as a specific marker for petersianum species of Biophytum genus and as a source of functional food ingredients as antioxidant agents to improve reproductive health.

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“…Briefly, the plant leaves were harvested, lyophylized, and extracted with ethanol 80 % using combination of maceration and sonication. The ethanolic extract was then fractionated using manual liquid-liquid sequential solvent extraction based on the method by (Maser et al, 2015) resulting in 4 fractions including hexane, chloroform, ethyl acetate and water fraction. The chloroform fraction was selected because the fraction showed the highest activity on inhibiting the viability of MCF-7 (data not shown).…”

Section: Origin and The Preparation Of The Plant Fractionsmentioning

confidence: 99%

“…Samples were subjected to LC-MS analysis. LC-MS system and the condition are similar to the study by (Maser et al, 2015). The identity of the compound was predicted based on fragmentation pattern of the mass spectra compared to literature.…”

Section: Lc-ms Analysis To Identify the Bioactive Compoundmentioning

confidence: 99%

BIOACTIVE COMPOUNDS FROM TORBANGUN [Plectranthus amboinicus (Lour.) SPRENG] CHLOROFORM FRACTION INDUCE APOPTOSIS IN BREAST CANCER (MCF-7 CELLS) IN VITRO

et al. 2017

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Torbangun (Plectranthus amboinicus (Lour.) Spreng) is a medicinal plant that has been traditionally used in tropical countries to cure various illnesses. The objective of this study was to identify the active compounds in the chloroform fraction which have effect on the apoptosis-related genes expression of breast cancer MCF-7 cells. Apoptosis was observed morphologically using Hoechst nuclear staining. Expression of the genes was analyzed using Real-Time PCR. Chemical compounds of the plant fractions were determined using LC-MS. Result of cell morphology observation clearly indicated apoptosis after the treatment of the plant fraction. Increased expression of anti-apoptotic gene Bcl-2 could not prevent the cells from apoptosis. Expressions of p53 and p21 genes were increased significantly. The expressions of caspase 9, caspase 7 and caspase 1 were increased at concentration-dependent manner. Most of the compounds in the chloroform fraction are identified as diterpenoids which may contribute to the apoptosis inducing activity of the fraction.

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Rapid Identification of Antibacterial Compounds from Turkey Berry by HPLC-Based Metabolomics

Cited by 11 publications

References 11 publications

Antibacterial stability of Spondias pinnata (L. f.) Kurz leaves extract and its mechanism

Antibacterial stability of Spondias pinnata (L. f.) Kurz leaves extract and its mechanism

Identification of antioxidant bioactive compounds as potential functional food ingredient from kebar grass (Biophytum petersianum) by metabolomic approach

BIOACTIVE COMPOUNDS FROM TORBANGUN [Plectranthus amboinicus (Lour.) SPRENG] CHLOROFORM FRACTION INDUCE APOPTOSIS IN BREAST CANCER (MCF-7 CELLS) IN VITRO

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