Rapid identification of bacterial mixtures in urine using MALDI-TOF MS-based algorithm profiling coupled with magnetic enrichment

Cheng, Wenmin; Shi, Haimei; Teng, Mengjing; Yu, Mei; Feng, Bin; Ding, Chuan‐Fan; Yu, Shaoning; Yang, Fan

doi:10.1039/d1an02098f

Cited by 10 publications

(6 citation statements)

References 33 publications

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“…Out of the MALDI MS instruments used, Microex of Bruker is the most extensively used instrument for microbial identication. [49][50][51][52][53] Fig. 2 represents the different sample preparation methods that have been in practice for the MALDI MS iden-tication of bacterial isolates.…”

Section: Methods Of Sample Preparationsmentioning

confidence: 99%

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A systematic assessment of matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) application for rapid identification of pathogenic microbes that affect food crops: delivered and future deliverables

et al. 2023

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Section: Methods Of Sample Preparationsmentioning

confidence: 99%

“…Out of the MALDI MS instruments used, Microflex of Bruker is the most extensively used instrument for microbial identification. 49–53…”

Section: Maldi Ms Spectrum-based Identification Of Microbial Pathogensmentioning

confidence: 99%

A systematic assessment of matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) application for rapid identification of pathogenic microbes that affect food crops: delivered and future deliverables

et al. 2023

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“…The Brucker MBT_FC. par method was selected to collect the data automatically [ 18 , 19 ]. The score standards for mass spectrometry identification were as follows: 2.300–3.000 for completely reliable identification at the species level; 2.00–1.99 for identification at the species level; 1.700–1.999 for identification at the genus level; 0.000–1.699 indicated no credible identification.…”

Section: Methodsmentioning

confidence: 99%

Rothia nasimurium as a Cause of Disease: First Isolation from Farmed Geese

Kang

Zhou

Jin

2022

Veterinary Sciences

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Rothia nasimurium was known previously as an opportunistic pathogen of animals. However, there are few reports regarding the pathogenicity of Rothia nasimurium. In September 2020, geese contracted a disease of unknown cause which brought economic losses to a farm in Jiangsu Province, China, prompting a series of investigations. The bacterium was isolated, cultured, and purified, and then identified using Gram staining, biochemical tests, matrix-assisted laser desorption/ionization time of flight mass spectrometry, and 16S rRNA sequence analysis. After determining the obtained bacteria species, antibiotic susceptibility tests and animal regression experiments were carried out. A strain of bacterium was successfully isolated from the livers of the diseased geese, which was identified as a strain of the Gram-positive bacterium Rothia nasimurium according to the 16S rRNA sequencing results. By indexing references, no goose was reported to have been infected with Rothia nasimurium. The antibiotic susceptibility testing showed that only four antibiotics (amikacin, cefazolin, fosfomycin, and ampicillin/sulbactam) could effectively inhibit the growth of the Rothia nasimurium strain. The animal regression experiments showed that the novel isolated strain could infect goslings, and it also causes serious depilation of goslings. The results of the manuscript expanded the range of pathogenic microorganisms in geese, which is helpful to develop methods for avian endemic control.

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“…Considering the unique relationship between PCa and urine biosamples, [42,43] urine is a noninvasive biofluid with unique advantages in detection scenarios. We thus performed MoS 2 /MXene-based UMF analysis on urinary biosamples from 60 PCa and 96 BPH patients (Figure 6a) and all relevant clinical information for biosamples was described in Table S2 (Supporting Information).…”

Section: Smf/umf Of Pca and Bph Via Mos 2 /Mxene Heterostructurementioning

confidence: 99%

Engineering the MoS₂/MXene Heterostructure for Precise and Noninvasive Diagnosis of Prostate Cancer with Clinical Specimens

et al. 2023

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High-throughput metabolic fingerprinting has been deemed one of the most promising strategies for addressing the high false positive rate of prostate cancer (PCa) diagnosis in the prostate-specific antigen (PSA) gray zone. However, the current metabolic fingerprinting remains challenging in achieving high-precision metabolite detection in complex biological samples (e.g., serum and urine). Herein, a novel self-assembly MoS 2 /MXene heterostructure nanocomposite with a tailored doping ratio of 10% is presented as a matrix for laser desorption ionization mass spectrometry analysis in clinical biosamples. Notably, owing to the two-dimensional architecture and doping effect, MoS 2 /MXene demonstrates favorable laser desorption ionization performance with low adsorption energy, which is evidenced by efficient urinary metabolic fingerprinting with an enhanced area under curve (AUC) diagnosis capability of 0.959 relative to that of serum metabolic fingerprinting (AUC = 0.902) for the diagnosis of PCa in the PSA gray zone. Thus, this MoS 2 /MXene heterostructure is anticipated to offer a novel strategy to precisely and noninvasively diagnose PCa in the PSA gray zone.

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Rapid identification of bacterial mixtures in urine using MALDI-TOF MS-based algorithm profiling coupled with magnetic enrichment

Abstract: Urinary tract infections (UTIs) are severe public health problem and caused by mono- or poly-bacteria. Culture-based methods are routinely used for the diagnosis of UTIs in clinical practice, but those...

Cited by 10 publications

References 33 publications

A systematic assessment of matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) application for rapid identification of pathogenic microbes that affect food crops: delivered and future deliverables

A systematic assessment of matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) application for rapid identification of pathogenic microbes that affect food crops: delivered and future deliverables

Rothia nasimurium as a Cause of Disease: First Isolation from Farmed Geese

Engineering the MoS₂/MXene Heterostructure for Precise and Noninvasive Diagnosis of Prostate Cancer with Clinical Specimens

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Rapid identification of bacterial mixtures in urine using MALDI-TOF MS-based algorithm profiling coupled with magnetic enrichment

Abstract: Urinary tract infections (UTIs) are severe public health problem and caused by mono- or poly-bacteria. Culture-based methods are routinely used for the diagnosis of UTIs in clinical practice, but those...

Cited by 10 publications

References 33 publications

A systematic assessment of matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) application for rapid identification of pathogenic microbes that affect food crops: delivered and future deliverables

A systematic assessment of matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) application for rapid identification of pathogenic microbes that affect food crops: delivered and future deliverables

Rothia nasimurium as a Cause of Disease: First Isolation from Farmed Geese

Engineering the MoS2/MXene Heterostructure for Precise and Noninvasive Diagnosis of Prostate Cancer with Clinical Specimens

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Resources

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Engineering the MoS₂/MXene Heterostructure for Precise and Noninvasive Diagnosis of Prostate Cancer with Clinical Specimens