Rapid identification of Burkholderia pseudomallei in blood culture supernatants by a coagglutination assay

Abstract

In total, 309 blood culture supernatants were tested for the presence of Burkholderia pseudomallei antigen using an in-house coagglutination test prepared by sensitising Cowan I staphylococcal cells with B. pseudomallei polyclonal antiserum. The coagglutination test gave a sensitivity, specificity, positive predictive value and negative predictive value of 100% in comparison with blood culture. A subset of 102 supernatants was also tested for B. pseudomallei antigen using a monoclonal antibody-based latex agglutination test. The sensitivity, specificity, positive predictive value and negative predictive values of this test were 100%, 90%, 75% and 100%, respectively.

Melioidosis: Indian perspective

“…Similar results are shown in a study from Vellore using in-house co-agglutination test and latex agglutination test [32]. In Thailand, the results of rapid immunofluorescence (IF) test and those of an existing IF method were prospectively compared with the culture of various clinical specimens from patients with suspected melioidosis.…”

Development of a simple method for the rapid identification of organisms causing anthrax by coagglutination test

“…But all these require expensive devices or complicated protocols [9] thus, are not suitable under routine laboratory conditions. CAT being an inexpensive, simple, sensitive and rapid test had been used for the diagnosis of various diseases [11,15]. Therefore, in the present study, an attempt was made to develop CAT as a sensitive, specific and easy-to-use technique for the rapid identification of bacteria having potential to produce anthrax.…”

“…In a previous study, we reported that detection of anthrax toxin by latex agglutination test (LAT) can be an effective technique for the diagnosis of anthrax as well as for the specific identification of cultures of organisms causing anthrax [10]. However, coagglutination test (CAT) is reported to be more sensitive, cheaper and simpler test [11]. Therefore, the present study was planned to evaluate whether CAT can serve as a surrogate for the specific identification of bacteria causing anthrax.…”

First report of Burkholderia pseudomallei ST412 and ST734 clones harbouring blaOXA-57 but susceptible to imipenem in India

“…Appropriate biochemicals were also set up [7]. The plates showed growth of non-haemolytic grey colonies at 24 h on sheep blood agar, which turned hazy β-haemolytic by 48 h. On MacConkey agar fine pale colonies formed at 24 h, and the colonies turned pink (lactose fermenting) by 48 h. The colonies were oxidase-positive and showed a 4 + reaction on agglutination with B. pseudomallei -specific antiserum raised in rabbits [8]. The preliminary screening media and other biochemicals confirmed the organism to be B. pseudomallei .…”