Rapid identification of Candida species by FT-IR microspectroscopy

Essendoubi, Mohammed; Toubas, D.; Bouzaggou, Mohamed; Pinon, J. M.; Manfait, Michel; Sockalingum, Ganesh D.

doi:10.1016/j.bbagen.2005.04.019

Cited by 95 publications

(73 citation statements)

References 28 publications

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“…This is conflictive with the results obtained by Essendoubi, M. et al [5] and in a blind trial we would wrongly consider two strains, one from each of these two clusters, as different species when in fact they are not.…”

Section: Resultscontrasting

confidence: 54%

“…[5,6] which may be resolved with their standardization and growth in controlled environment. This is in fact very important for the reproducibly of the spectroscopic analysis of microorganisms [5].…”

Section: Introductionmentioning

confidence: 99%

“…This process is a fast, non-destructive analytical method and the fingerprint is reproducible [4,5] allowing the rapid distinction of genera and species with low cost from a small amount of analytical biomass [5,6]. It also allows the phonotypical typing and characterization of microbial species, strains, biochemical behaviors and adaptation mechanisms and can even be used to identify certain macromolecules (nature, quantity and conformation) present in the cells [5]. A fast response is important for the medical community, especially when considering that Candida is the 4 th most frequently isolated microorganism from nosocomial infections [7].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Assessing the Potential of FT-IR to Identify Clinical Strains of Candida spp

Brandão¹,

Veríssimo²,

Brandão³

2011

TOAINFOJ

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Section: Resultscontrasting

confidence: 54%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Assessing the Potential of FT-IR to Identify Clinical Strains of Candida spp

Brandão¹,

Veríssimo²,

Brandão³

2011

TOAINFOJ

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“…This led to a large number of studies with different tissues and cell lines with an objective to make the technique relevant to oncology (Fukuyama et al 1999, Diem et al 2000, Gao et al 1999, Yano et al 2000, Malins et al 2003. The potential application in other biomedical fields was expanded by studying the classification of microorganisms using the technique and using it for identifying pathogens , Choosmith et al 2001, Sandt et al 2003, Essendoubi et al 2005. These studies led to the development of the concept of "biomarkers" which are parameters or statistics derived from the spectral data that help to identify or differentiate among samples.…”

Section: Brief Historical Perspective Of Ftir Spectroscopy In Biomedimentioning

confidence: 99%

Chemometrics of Cells and Tissues Using IR Spectroscopy - Relevance in Biomedical Research

Sahu¹,

Mordechaǐ²

2012

Infrared Spectroscopy - Life and Biomedical Sciences

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“…Identification of microorganisms using cultivation and microscopic examination is time consuming and alone does not provide sufficient information with respect to the evaluation of health hazards in connection with bioaerosol exposure. FT-IR spectroscopy has widely been used for the characterization and identification of bacteria and yeasts, due to the fact that they are hydrophilic microorganisms and can easily be suspended in water for sample preparation (Essendoubi et al, 2005;Duygu et al, 2009). The identification of airborne fungi using FT-IR spectroscopy was described by Fischer and coworkers.…”

mentioning

confidence: 99%

Application of FTIR Spectroscopy in Environmental Studies

Simonescu¹

2012

Advanced Aspects of Spectroscopy

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Figure 1. Schematic representation of the electromagnetic spectrum (adapted from http://www2.chemistry.msu.edu/faculty/reusch/VirtTxtJml/Spectrpy/UV-Vis/spectrum)? Advanced Aspects of Spectroscopy 50Infrared radiation is divided into:near (NIR, ν = 10,000 -4,000 cm -1 ); -middle (MIR, ν = 4,000 -200 cm -1 ) and -far (FIR, ν = 200 -10 cm -1 ).Because all compounds show characteristic absorption/emission in the IR spectral region and based on this property they can be analyzed both quantitatively and qualitatively using FT-IR spectroscopy.Today FT-IR instruments are digitalized and are faster and more sensitive than the older ones. FT-IR spectrometers can detect over a hundred volatile organic compounds (VOC) emitted from industrial and biogenic sources. Gas concentrations in stratosphere and troposphere were determined using FT-IR spectrometers (Puckrin et al., 1996).In case of environmental studies FTIR Spectroscopy is used to analyze relevant amount of compositional and structural information concerning environmental samples (Grube et al., 2008). The analysis can be performed also to determine the nature of pollutants, but also to determine the bonding mechanism in case of pollutants removal by sorption processes. Techniques for measuring gas pollutants such as continuous air pollutants analyzer (SO2, NO2, O3, NH3), on-line gas chromatography (GC) used simple real-time instruments to quantify gas pollutants. They need to use several sensors in order to analyze multiple gas pollutants simultaneously.FT-IR spectroscopy coupled with other spectroscopic techniques such as AAS (atomic absorption spectroscopy) have been used to assess the impact of industrial and natural activities on air quality (Kumar et al., 2005;Childers et al., 2001).In addition to the traditional transmission FTIR (T-FTIR) methods (e.g. KBr-pellet or mull techniques), modern reflectance techniques are widely used today in environmental, agricultural, pharmaceuticals, and food studies. These modern techniques are attenuated total reflection FTIR (ATR-FTIR), and diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS). The choice of the method to be used depends on many factors such as: the information needed (bulk versus surface analysis), the physical form of the sample, the time required for sample preparation (Majedová et al., 2003).In the following there will be presented some of the most important research studies related to the involvement of FTIR spectroscopy in environmental studies. Traditional transmission FT-IR (T-FTIR) spectroscopy in environmental studiesTransmission spectroscopy is the oldest and most commonly used method for identifying either organic or inorganic chemicals providing specific information on molecular structure, chemical bonding and molecular environment. It can be applied to study solids, liquids or gaseous samples being a powerful tool for qualitative and quantitative studies. Application of FTIR Spectroscopy in Environmental Studies 51FTIR instrument's principle of function is the following: IR radi...

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Rapid identification of Candida species by FT-IR microspectroscopy

Cited by 95 publications

References 28 publications

Assessing the Potential of FT-IR to Identify Clinical Strains of Candida spp

Assessing the Potential of FT-IR to Identify Clinical Strains of Candida spp

Chemometrics of Cells and Tissues Using IR Spectroscopy - Relevance in Biomedical Research

Application of FTIR Spectroscopy in Environmental Studies

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