2003
DOI: 10.1128/jcm.41.12.5660-5664.2003
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Rapid Identification of Commonly EncounteredCandidaSpecies Directly from Blood Culture Bottles

Abstract: We report a rapid-cycle, real-time PCR method for identifying six Candida spp. directly from BACTEC blood culture bottles. Target sequences in the noncoding internal transcribed spacer regions of the rRNA operon were simultaneously amplified and interrogated with fluorescent probes to identify Candida albicans, C. glabrata, C. parapsilosis, C. tropicalis, C. krusei, and C. lusitaniae; these account for 88% of the yeast species isolated from positive blood cultures in our laboratory. Any of the first four speci… Show more

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Cited by 61 publications
(37 citation statements)
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“…Detection of oligonucleotide probe hybridization has been reported using microtitration plate-based enzyme immunoassay (5,17,30), Southern or slot blotting (4,6,23,28), and fluorogenic probes (8,19,24). We chose the RLB format, given the advantages of relative simplicity, low cost, ready availability of the materials and the methods, and ability to simultaneously analyze multiple specimens against multiple probes (7).…”
Section: Discussionmentioning
confidence: 99%
“…Detection of oligonucleotide probe hybridization has been reported using microtitration plate-based enzyme immunoassay (5,17,30), Southern or slot blotting (4,6,23,28), and fluorogenic probes (8,19,24). We chose the RLB format, given the advantages of relative simplicity, low cost, ready availability of the materials and the methods, and ability to simultaneously analyze multiple specimens against multiple probes (7).…”
Section: Discussionmentioning
confidence: 99%
“…Rapid, reliable tests that identify a majority of species known to cause infections would greatly improve patient treatment. Many of the recently developed molecular-based assays are real-time assays that provide rapid results, but they are often limited in the number of species that can be detected in a single assay (18). Standard PCR-based assays are also limited in the number of species that can be identified from a single amplification.…”
mentioning
confidence: 99%
“…Current phenotypic methods for yeast identification, including colony morphology, germ tube test, urease activity, and the API 20C AUX strip (bioMeriuex, Durham, NC), take an additional 48 to 72 h to complete after the yeast-like fungal pathogens are observed in the blood culture media (33). Various molecular techniques, including real-time PCR, fluorescent in situ hybridization, and pyrosequencing, have been developed to speed the identification of blood-borne fungi (14,23,25), but they have not been implemented as routine techniques in the clinical microbiology laboratory.…”
mentioning
confidence: 99%