Rapid Identification of Drug Resistance and Phylogeny in M. tuberculosis, Directly from Sputum Samples

Abstract

The COVID-19 pandemic negatively affected the progress in accessing essential Tuberculosis (TB) services and reducing the burden of TB disease, resulting in a decreased detection of new cases and increased deaths. Generating molecular diagnostic tests with faster results without losing reliability is considered a priority.

Spotlight on mycobacterial lipid exploitation using nanotechnology for diagnosis, vaccines, and treatments

“… 79. , 80. Therefore, the development of nanotechnology-based sequencing methods to address this approach constitutes a worthy challenge.…”

Two-stage tuberculosis diagnostics: combining centrifugal microfluidics to detect TB infection and Inh and Rif resistance at the point of care with subsequent antibiotic resistance profiling by targeted NGS

“…30 Other approaches include tNGS by either selective enrichment or targeted amplification. 30,31 However, approaches are still evolving, with selective enrichment facing difficulties in achieving comprehensive target coverage, 32 and proposed amplification panels being limited in the genomic regions to be analysed. 33 Therefore, we aim to establish a tNGS amplification approach encompassing the full panel of genomic regions known or suspected to be involved in antibiotic resistance of MTBC strains.…”

The MAGMA pipeline for comprehensive genomic analyses of clinical Mycobacterium tuberculosis samples

“…Recent approaches have aimed to circumvent this by enriching Mtb DNA in vitro or in silico . In vitro methods include enzymatic removal of non- Mtb contaminants [ 7 ], Mtb -specific DNA-capture probes [ 8 , 9 ] or multiplex-amplification of drug resistance-conferring regions [ 10 , 11 ]. Mtb enrichment in silico can be achieved by classifying reads and rejecting any reads not classified as Mtb [ 12 ].…”