Rapid identification of enantioselective ketone reductions using targeted microbial libraries

Highly enantioselective bioreduction of N-methyl-3-oxo-3-(thiophen-2-yl) propanamide for the production of (S)-duloxetine

“…Using a targeted microbial library (Homann et al 2004), we tested 38 yeast strains isolated from liquor pits (Supplementary Table S1). Rhodotorula sp.…”

Novel Developments Employing Redox Enzymes: Old Enzymes in New Clothes

“…For efficient screening, readily prepared libraries of microbial strains are used to identify suitable microorganisms quickly [31][32][33][34][35][36][37]. Besides fungi, yeasts, and bacterial strains, algae and plant cells proved to be applicable (Table 1, entries 2 and 5, respectively) [38].…”

Biotransformations in Small‐Molecule Pharmaceutical Development

“…Numerous other microorganisms also produce the ADH enzymes (KREDs) responsible for asymmetric ketone reduction, and so suitable biocatalysts have traditionally been identified by extensive microbial screening. Homann et al 145 have recently reported the identification of a subset of 60 ADH-producing microbial cultures that cut microbial screening time from weeks to days.…”