Rapid identification of furanocoumarins in Angelica dahurica using the Online LC‐MMR‐MS and their nitric oxide inhibitory activity in RAW 264.7 cells

Abstract

The results obtained showed that the anti-inflammatory activities of A. dahurica could be linked to imperatorin and phellopterin.

Simultaneous Characterisation of Fifty Coumarins from the Roots ofAngelica dahuricaby Off-line Two-dimensional High-performance Liquid Chromatography Coupled with Electrospray Ionisation Tandem Mass Spectrometry

“…In the literature many coumarins in A. dahurica have been investigated by HPLC, HPLC‐NMR‐MS, and HPLC ESI‐MS (Kang et al ., ; Kang et al ., ; Zheng et al ., ; Park et al ., ; Zhang et al ., ). However, there is no report on the characterization of trace amounts of coumarins in A. dahurica by using off‐line 2D‐HPLC–ESI/MS n .…”

Modern Approaches in the Search for New Active Compounds from Crude Extracts of Natural Sources

“…NO inhibitory activities for five main fractions of the extract were evaluated; these fractions were identified by LC-NMR-MS as containing furanocoumarins: byakangelicol, oxypeucedanin, imperatorin, phellopterin, and isoimperatorin. The results obtained showed that the anti-inflammatory activities of A. dahurica could be linked to imperatorin and phellopterin [112].…”

“…Recently, Kang et al used LC-NMR-MS and NO inhibitory assays to investigate the anti-inflammatory constituents of extracts from Angelica dahurica, a plant used for the treatment of colds, headaches, and toothaches in Korean traditional medicine [112]. Reversed-phase isocratic chromatography was performed using the acetonitrile-water solvent system on a C 30 column.…”

Dose-dependent targeted knockout methodology combined with deep structure elucidation strategies for Chinese licorice chemical profiling

“…Effluent collected in the first 5 min under 2% B and labeled as fraction 0 was used to flush the strong polar compounds, such as monosaccharides, polysaccharides and amino acids, to reduce interference in the MS and NMR signals. The remaining 20 fractions (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20) were collected from the p-HPLC every 3 min. Data were processed by ChemStation B.04.03 software.…”

“…In order to characterize the chemical profiling of TCM, hyphenated techniques, including LC-MS [6][7][8], GC-MS [9][10][11], CE-DAD and/or MS [12][13][14], and LC-NMR [15], have been widely employed. Among these hyphenated techniques, ultra-performance liquid chromatography coupled with quadrupole-time-of-flight MS (UPLC-Q/TOF MS) is the most feasible method for rapid chemical profiling in TCM [16].…”