Rapid identification of Paracoccidioides lutzii and P. Brasiliensis using Fourier Transform Infrared spectroscopy

Filho, Olavo O. Comparato; Morais, Flavia Villaça; Bhattacharjee, Tanmoy; Castilho, Maiara L.; Raniero, Leandro

doi:10.1016/j.molstruc.2018.09.016

Cited by 8 publications

(4 citation statements)

References 28 publications

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“…The identification of species boundaries and introgression in fungal pathogens has human health-related implications. Paracoccidioides lutzii and P. brasiliensis sensu stricto show differences in the immunological response they elicit ( 14 , 32 ), the strength of the disease they cause ( 14 ), and in traits involved in diagnostic tools ( 33 – 35 ). Introgression, then, can be a vehicle to transfer virulence factors and antifungal resistance in Paracoccidioides .…”

Section: Discussionmentioning

confidence: 99%

Paracoccidioides Genomes Reflect High Levels of Species Divergence and Little Interspecific Gene Flow

Mavengere

Mattox

Teixeira

et al. 2020

mBio

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Section: Discussionmentioning

confidence: 99%

Paracoccidioides Genomes Reflect High Levels of Species Divergence and Little Interspecific Gene Flow

Mavengere

Mattox

Teixeira

et al. 2020

mBio

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“…[ 151 , 152 ]. Comparato Filho et al [ 153 ] used the vibrational spectroscopy signatures of proteins, polysaccharides, and nucleic acids to differentiate P. brasiliensis and P. lutzii successfully. They could visualize the P. brasiliensis complex’s genetic diversity but could not define parameters for their differentiation.…”

Section: Molecular Diagnosis: Where Do We Stand?mentioning

confidence: 99%

“…The test does not require extensive sample preparation, such as MALDI-ToF, cutting valuable time off the diagnostic pipeline. On the other hand, it has some challenging technical requirements, possibly affecting reproducibility [ 153 ].…”

Section: Molecular Diagnosis: Where Do We Stand?mentioning

confidence: 99%

Molecular Tools for Detection and Identification of Paracoccidioides Species: Current Status and Future Perspectives

Pinheiro

Hahn

Camargo

et al. 2020

JoF

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Paracoccidioidomycosis (PCM) is a mycotic disease caused by the Paracoccidioides species, a group of thermally dimorphic fungi that grow in mycelial form at 25 °C and as budding yeasts when cultured at 37 °C or when parasitizing the host tissues. PCM occurs in a large area of Latin America, and the most critical regions of endemicity are in Brazil, Colombia, and Venezuela. The clinical diagnosis of PCM needs to be confirmed through laboratory tests. Although classical laboratory techniques provide valuable information due to the presence of pathognomonic forms of Paracoccidioides spp., nucleic acid-based diagnostics gradually are replacing or complementing culture-based, biochemical, and immunological assays in routine microbiology laboratory practice. Recently, taxonomic changes driven by whole-genomic sequencing of Paracoccidioides have highlighted the need to recognize species boundaries, which could better ascertain Paracoccidioides taxonomy. In this scenario, classical laboratory techniques do not have significant discriminatory power over cryptic agents. On the other hand, several PCR-based methods can detect polymorphisms in Paracoccidioides DNA and thus support species identification. This review is focused on the recent achievements in molecular diagnostics of paracoccidioidomycosis, including the main advantages and pitfalls related to each technique. We discuss these breakthroughs in light of taxonomic changes in the Paracoccidioides genus.

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“…A precise assessment of the range and opportunity for hybridization will be crucial to establish the genetic, environmental, and demographic factors that govern the patterns of introgression in Paracoccidioides.The identification of species boundaries and introgression in fungal pathogens has human health-related implications. Paracoccidioides lutzii and P. brasiliensis sensu stricto show differences in the immunological response they elicit(Lenhard-Vidal et al 2013;Siqueira et al 2016), the strength of the disease they cause(Siqueira et al 2016), and in traits involved in diagnostic tools(De Almeida et al 2015;Arantes et al 2017;Comparato Filho et al 2019). Introgression, then, can be a vehicle to transfer virulence factors and antifungal resistance in Paracoccidioides.…”

mentioning

confidence: 99%

Paracoccidioidesgenomes reflect high levels of species divergence and little interspecific gene flow

Mavengere

Mattox

Teixeira

et al. 2020

Preprint

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The fungus Paracoccidioides spp. is a prevalent human pathogen endemic to South America. The genus is composed by five species but most of the inference of their evolutionary history has used only a handful of nuclear markers. In this report, we use 37 whole genome sequences to study the allocation of genetic variation in Paracoccidioides. We tested predictions of advanced speciation and find support for strong divergence in the Paracoccidioides genus. We find that the five species of Paracoccidioides are reciprocally monophyletic and that their genomes confirm a history of deep divergence. We also find that the amount of gene flow between species is low and in some cases even completely absent in spite of geographic overlap. Our results indicate that the genomes of the five species of Paracoccidioides are highly differentiated and that should differentiation has not been hampered by gene exchange.

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Rapid identification of Paracoccidioides lutzii and P. Brasiliensis using Fourier Transform Infrared spectroscopy

Cited by 8 publications

References 28 publications

Paracoccidioides Genomes Reflect High Levels of Species Divergence and Little Interspecific Gene Flow

Paracoccidioides Genomes Reflect High Levels of Species Divergence and Little Interspecific Gene Flow

Molecular Tools for Detection and Identification of Paracoccidioides Species: Current Status and Future Perspectives

Paracoccidioidesgenomes reflect high levels of species divergence and little interspecific gene flow

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