Rapid identification of subgenera of human adenovirus by serological and PCR assays

Akalu, Abebe; Seidel, W; Liebermann, Herbert; Bauer, Ulrike; Döhner, L

doi:10.1016/s0166-0934(97)00213-9

Cited by 21 publications

(22 citation statements)

References 25 publications

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“…As observed for other parts of the genome (2,3,6,7,20 ), the sequences were nearly identical within each species but varied with respect to the other species. On the basis of the generated sequence information, we chose 5 primer pairs that flanked the selected probe target sequence.…”

Section: Discussionsupporting

confidence: 52%

Development of a PCR-Based Assay for Detection, Quantification, and Genotyping of Human Adenoviruses

Chmielewicz¹,

Nitsche²,

Schweiger³

et al. 2005

Clinical Chemistry

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Section: Discussionsupporting

confidence: 52%

Development of a PCR-Based Assay for Detection, Quantification, and Genotyping of Human Adenoviruses

Chmielewicz¹,

Nitsche²,

Schweiger³

et al. 2005

Clinical Chemistry

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“…10,11 In the past hemagglutination properties of human adenoviruses have been employed for serotyping in clinical routine, but nowadays PCR analysis and the avaliability of type-specific antibodies have superseded this technique. 12 The interest in adenoviral fiber proteins has gained a renaissance since it has been shown that this protein is mainly responsible for cell tropism of adenoviral vectors. 13 Whether the described affinity between erythrocytes and adenoviruses could influence gene transfer efficiency, biodistribution or immunological side effects remains unclear.…”

Section: Hemagglutinationmentioning

confidence: 99%

Titer determination of Ad5 in blood: a cautionary note

Cichon

Boeckh-Herwig

Kuemin³

et al. 2003

Gene Ther

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Recombinant adenoviruses are presently the most efficient in vivo gene transfer system available. Targeting single organs or large tumors by adenoviral vectors requires an intravascular route of application. During the first pass of viral particles through the vascular bed of the target tissue, virus uptake is not quantitative and undefinite amounts of particles leak into circulation. To determine the amount of leaking particles and to calculate organ-specific uptake (in-/outflow ratio), it is necessary to titrate virus particles directly in blood. In preclinical and clinical trials titration is currently mostly done with blood plasma instead of full blood. However, this technique provides valid results only as long as there is no affinity between adenovirus particles and erythrocytes. In this study we demonstrate that Ad5 particles, as mostly employed for gene therapy, have a strong affinity to human erythrocytes. At 60 min after coincubation of human erythrocytes and Ad5 particles, more than 98% of the particles are attached to the surface of erythrocytes. Therefore, ignoring the amount of red cell bound particles by performing titration in plasma leads to severe miscalculation of organ-specific transfer rates or virus circulation half-life. The biological impact of an increased affinity between virus particles and erythrocytes will be discussed.

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“…The PCR primer sets used for the detection of rotavirus, norovirus, astrovirus, and enteric adenovirus were described previously (1,10,12). The RT-PCR for RNA viruses and the PCR for DNA virus were performed as described earlier (1,10,12).…”

mentioning

confidence: 99%

Molecular Epidemiology and Clinical Manifestations of Viral Gastroenteritis in Hospitalized Pediatric Patients in Northern Taiwan

et al. 2007

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By reverse transcription-PCR or PCR, among 257 children with nonbacterial acute gastroenteritis (AGE), rotavirus, norovirus, astrovirus, enteric adenovirus, and multiple viruses were identified in 78 (30.4%), 21 (8.2%), 7 (2.7%), 51 (19.8%), and 53 (20.6%) patients, respectively. Higher disease severity was found for AGE caused by multiple viruses and by rotavirus alone. The majority of rotaviruses isolated from 2004 to 2006 belonged to genotypes G1 (20.4%), G2 (16.5%), G3 (27.2%), and G9 (21.4%).

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Rapid identification of subgenera of human adenovirus by serological and PCR assays

Cited by 21 publications

References 25 publications

Development of a PCR-Based Assay for Detection, Quantification, and Genotyping of Human Adenoviruses

Development of a PCR-Based Assay for Detection, Quantification, and Genotyping of Human Adenoviruses

Titer determination of Ad5 in blood: a cautionary note

Molecular Epidemiology and Clinical Manifestations of Viral Gastroenteritis in Hospitalized Pediatric Patients in Northern Taiwan

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