Rapid identification of triptolide in Tripterygium wilfordii products by gold immunochromatographic assay

Duan, Yaping; Luo, Jiaoyang; Liu, Congmin; Shan, Linan; Dou, Xiaobing; Yang, Shihai; Yang, Meihua

doi:10.1016/j.jpba.2019.02.020

Cited by 6 publications

(2 citation statements)

References 25 publications

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“…The assay commences when the liquid containing the analyte of interest flows through the strip after sample application. The sets of the strip can be provided with the cassette, which contains the sample loading opening that is connected to the sample pad and read-out opening [37][38][39][40][41][42][43]. However, the cassette is not required in certain assays.…”

Section: Competitive Formatmentioning

confidence: 99%

“… [ 33 ] Mitragynine Indole alkaloid Mitragyna speciosa [ 99 , 101 ] Salvinorin A Diterpenoid Salvia divinorum [ 100 ] Saikosaponin d Triterpenoid glycoside Bupleurum falcatum [ 39 ] Icariin Flavone glycoside Epimedium spp. [ 40 ] Isomiroestrol Chromene Pueraria mirifica [ 104 ] Deoxymiroestrol Chromene Pueraria mirifica [ 64 ] Triptolide Diterpenoid epoxide Tripterygium wilfordii [ 38 ] Aristolochic acid I Nitrophenanthrene Aristolochia spp. [ 47 ] Aconitine Alkaloid Aconitum spp.…”

Section: Introductionmentioning

confidence: 99%

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Lateral flow immunoassay for small-molecules detection in phytoproducts: a review

et al. 2022

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Phytoproducts are involved in various fields of industry. Small-molecule (Mw < 900 Da) organic compounds can be used to indicate the quality of plant samples in the perspective of efficacy by measuring the necessary secondary metabolites and in the perspective of safety by measuring the adulterant level of toxic compounds. The development of reliable detection methods for these compounds in such a complicated matrix is challenging. The lateral flow immunoassay (LFA) is one of the immunoassays well-known for its simplicity, portability, and rapidity. In this review, the general principle, components, format, and application of the LFA for phytoproducts are discussed.

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Section: Competitive Formatmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Lateral flow immunoassay for small-molecules detection in phytoproducts: a review

et al. 2022

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Novel identification of mAbs by Raman spectroscopy

Duan,

Liu,

Wang

et al. 2024

Engineering Reports

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The identification of the critical quality attributes (CQAs) of monoclonal antibodies (mAbs) is a key component of quality by design. Traditional detection methods for the identification of mAbs, such as peptide mapping and weak cation‐exchange chromatography (WCX‐HPLC), require sophisticated equipment, experienced staff, and a considerable amount of time. In this study, the novel identification of mAbs was performed using Raman spectroscopy, combined with the evaluation of other CQAs, such as the appearance and purity of size‐exclusion high‐performance liquid chromatography(SEC‐HPLC), charge heterogeneity of WCX‐HPLC, and bioassay. Raman spectroscopy achieved comparable results to the conventional, but complex, approach to identifying anti‐vascular endothelial growth factor antibodies. Raman spectroscopy was used to identify and distinguish between different antibody types. Additionally, the Raman technique with principal component analysis of multivariate algorithms is rapid, efficient, and accurate for mAb identification; this technique has great potential to support biopharmaceutical development and counterfeiters.

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Preparation of sensitive monoclonal antibodies against triptolide and establishment of a rapid and cost-effective icELISA method for authentication of Tripterygium wilfordii and related products

Duan

Liu

Dou

et al. 2020

Microchemical Journal

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Rapid identification of triptolide in Tripterygium wilfordii products by gold immunochromatographic assay

Cited by 6 publications

References 25 publications

Lateral flow immunoassay for small-molecules detection in phytoproducts: a review

Lateral flow immunoassay for small-molecules detection in phytoproducts: a review

Novel identification of mAbs by Raman spectroscopy

Preparation of sensitive monoclonal antibodies against triptolide and establishment of a rapid and cost-effective icELISA method for authentication of Tripterygium wilfordii and related products

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