Rapid immunological detection of copper ions using fluorescence immunochromatographic assay

Abstract

One-step fluorescence immunochromatographic assay (FICA) has been developed using fluorescent microspheres-labelled monoclonal antibody (McAb) probe for the rapid detection of copper ions in herbal plants and soil samples were investigated in this study. The results can be read by the fluorescence reader according to the fitted standard curve equation. Under optimal conditions, detecting copper ions with FICA can be done within 15 min with a LOD (limit of detection) of 0.2 μg/L, and was very useful for the rapid onsite testing. A total of 31 solid samples were pre-treated by microwave digestion combined with cation exchange solid phase extraction and prepared as test samples for FICA and ICP-AES. The two methods showed good relativity with correlation coefficients of 0.924 and 0.969 in herbal plants and soil samples for copper detection, respectively. We hope that our research can help promote the daily monitoring of heavy metal pollution effectively.

Development of two highly sensitive and selective sensor-assisted fluorescence immunoassays for trace determination of copper residues in food samples

“…Kinetic exclusion assays (KinExA) employing KinExA™ immunosensor offered the same advantages of FIA besides the convenience of automated analysis. Fluorescence immunochromatographic assay has been described for determination of copper ions in herbal plants and soil samples; 31 however, the assay has not been validated for food samples, and it suffers from the procedure convenience. KinExA has not been described yet for copper ions in food samples.…”

Dual-emission fluorescent nanoprobe based on Ag nanoclusters for sensitive detection of Cu(II)

“…It is of great significance to detect the content of heavy metal ions, nowadays, the existing methods of copper ion detection include flame atomic absorption spectrometry [15][16][17], ion chromatography [18], molecular spectroscopy [19,20] and so on, among them, fluorescence spectroscopy has attracted more and more attention from scientists in recent years [2,[21][22][23][24][25][26], because of its striking characteristics such as simple instrument and equipment, short analysis time, the fluorescence signal can be observed by naked eyes and convenient semi-quantitative analysis. The semi-quantitative analysis of metal ions by fluorescence spectrometry is mainly based on the phenomenon that metal ions can quench or enhance the fluorescence specificity of fluorescent molecular probes [27].…”

Rapid and sensitive detection of ochratoxin A in rice flour using a fluorescent microsphere immunochromatographic test strip assay

“…The antibody is chemically bound to the FM surface to provide a high degree of long-term stability in sample testing (Huang et al, 2017). Fluorescent immunochromatography is widely used in the food safety industry (Duan et al, 2017;Yang et al, 2018). The combination of FM and immunochromatographic test strip (ICTS) (FM-ICTS) and a fluorescence analyzer make it possible to quantitatively read and analyze fluorescence signals and avoids errors caused by visual observation.…”