Rapid immunophenotypic characterization of chromosomally aberrant cells by the new FICTION method

Weber‐Matthiesen, Klaus; Deerberg, J.; Müller-Hermelink, A; Schlegelberger, Brigitte; Grote, W.

doi:10.1159/000133515

Cited by 55 publications

(24 citation statements)

References 2 publications

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“…7b,d,f). By combining immunofluorescent staining for cyclin B1 with FISH for t(11;14) (FICTION methodology) 17 it was found that many of the cyclin B1 positive cells contained the fusion signal and thus belonged to the tumor cell population (data not shown).…”

Section: Validation Of the Differential Gene Expression In Another Sementioning

confidence: 99%

Mantle cell lymphomas with low levels of cyclin D1 long mRNA transcripts are highly proliferative and can be discriminated by elevated cyclin A2 and cyclin B1

Sander

Flygare

Porwit‐MacDonald

et al. 2005

Intl Journal of Cancer

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The role of transcript variants of cyclin D1 in cancer biology is unclear. Most tumors with high levels of cyclin D1 express 2 transcripts due to alternative splicing: one full-length transcript of 4.4 kb and one short transcript of approximately 1.7 kb. The short transcript lacks part of the 3 0 UTR region regulating mRNA stability and has a longer half-life. In our study, the contribution of each of these mRNAs to gene expression and cell proliferation has been investigated in mantle cell lymphoma (MCL), a B cell lymphoma characterized by a specific gene translocation resulting in enhanced expression of cyclin D1. A subset of MCL tumors with low levels of the long cyclin D1 transcript (cyclin D1 3 0 UTR) was identified by quantitative PCR and by oligonucleotide array hybridization. This tumor-subset had 3.4-fold higher levels of the short form of cyclin D1 mRNA (p < 0.0001) and had higher expression of cyclin D1 protein. Gene expression analysis identified a number of cell-cycle regulatory genes as upregulated. There was a significant difference in frequencies of cyclin B1 (p 5 0.0006) and cyclin A2 (p 5 0.0006) positive cells that discriminated MCL with low cyclin D1 3 0 UTR from other highly proliferative MCL. Among differentially expressed genes, there was a highly upregulated gene with homology to the group of cell-cycle promoting E2F transcription partners, E2F_TDP5. Several of the upregulated genes, such as TOP2A, AURORA A and RRM2 may influence a response to therapy. Identification of MCL with low cyclin D1 3 0 UTR is important because it seems to be associated with shorter overall survival. ' 2005 Wiley-Liss, Inc.Key words: mantle cell lymphoma; cyclins; CCND1 3 0 UTR; proliferation; expression profiling; micro array In most tumors demonstrating inappropriately high levels of cyclin D1 gene expression, 2 cyclin D1 transcripts are present due to alternative polyadenylation: one full-length transcript of 4.4 kb and one shorter transcript of 1.7 kb.1 The short transcript lacks the 3 0 -UTR, AU-rich, sequences of the mRNA containing destabilizing elements (Fig. 1). The role of long and short cyclin D1 transcript in cancer biology is unclear. The aberrant expression of cyclin leads to a disturbed cell cycle regulation; cyclin D1 binds to cyclin dependent kinase Cdk4 and initiates phosphorylation of Rb resulting in the release of E2F and transcriptional activation of S-phase genes.2 It has also been suggested that cyclin D1 sequesters Cip/Kip cell cycle inhibitors such as p21waf1 and p27Kip1 from cyclinE/Cdk2 complexes thereby relieving the inhibitory effect and further inducing progression toward S-phase.3 In addition to the cell cycle related functions, cyclin D1 is a transcriptional activator 2 that induces gene expression not only via the E2F transcription factors but also by modifying the effects of transcription factors such as C/EBP beta 4 and other targets, not yet wellcharacterized. 2Mantle cell lymphoma (MCL) is a non-Hodgkin lymphoma of B cell origin that expresses the B cell markers CD20 and CD7...

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Section: Validation Of the Differential Gene Expression In Another Sementioning

confidence: 99%

Mantle cell lymphomas with low levels of cyclin D1 long mRNA transcripts are highly proliferative and can be discriminated by elevated cyclin A2 and cyclin B1

Sander

Flygare

Porwit‐MacDonald

et al. 2005

Intl Journal of Cancer

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“…The MAC method was based on the techniques of Stenman et al (1975), who used immunostaining on cytospin preparations. Several MAC modifications and related techniques have emerged since 1984 (Anastasi et al, 1991;Haas et al, 1987;Henn et al, 1990;Kibbelaar et al, 1992;Köller et al, 1986Köller et al, , 1989Mullink et al, 1989;Perry & Thomson, 1986;Price et al, 1992;Schlegelberger et al, 1992;van den Berg et al, 1991;Weber-Matthiesen et al, 1993a. For phenotypic analysis some of these employ only cytochemical techniques (Anastasi et al, 1991) and others use only immunocytochemical techniques .…”

Section: Hodgkin's Lymphoma (Hl)mentioning

confidence: 99%

Lineage Specificity in Haematological Neoplasms

Knuutila

1997

Br J Haematol

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“…In 1992, Weber-Matthiesen et al (7,8) developed a method called Fluorescence Immuno-phenotyping and Interphase Cytogenetics as a Tool of the Investigation of Neoplasm. However, based on images presented in those reports, it was considered that improvements in the technique used for detection were required and it has yet to become a universal method.…”

Section: Double-detection -------------------------------------------mentioning

confidence: 99%

Novel and simple method of double‑detection using fluorescence inï¿½situ hybridization and fluorescence immunostaining of formalin‑fixed paraffin‑embedded tissue sections

Ikeda

2017

Oncol Lett

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Abstract. The importance of fluorescence in situ hybridization (FISH) for pathological diagnosis has been increasing. However, the procedures utilized for a conventional FISH method with formalin-fixed paraffin-embedded tissue sections are complicated and it is difficult to perform as a routine laboratory test. In addition, there are difficulties with differentiation of targeted cells in observations with a fluorescence microscope. The present study reported a novel method that utilizes FISH in combination with fluorescence immunostaining as a simple double-detection technique that addresses these problems. Using this novel method, various genetic aberrations, as well as protein overexpression were easily visualized in isologous sections. In particular, FISH signals with our method clearly identify target cells in samples with poor differentiation between tumor cells coexisting with normal cells. It is proposed that this simple technique is widely applicable as a routine laboratory test and future developments are expected.

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Rapid immunophenotypic characterization of chromosomally aberrant cells by the new FICTION method

Cited by 55 publications

References 2 publications

Mantle cell lymphomas with low levels of cyclin D1 long mRNA transcripts are highly proliferative and can be discriminated by elevated cyclin A2 and cyclin B1

Mantle cell lymphomas with low levels of cyclin D1 long mRNA transcripts are highly proliferative and can be discriminated by elevated cyclin A2 and cyclin B1

Lineage Specificity in Haematological Neoplasms

Novel and simple method of double‑detection using fluorescence inï¿½situ hybridization and fluorescence immunostaining of formalin‑fixed paraffin‑embedded tissue sections

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