Rapid impedance detection of salmonellas in confectionery using modified LICNR broth

Bullock, R.D.; Frodsham, D.

doi:10.1111/j.1365-2672.1989.tb05107.x

Cited by 26 publications

(8 citation statements)

References 7 publications

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“…Previous studies have shown that the impedance-based microbiological method is a very sensitive and reliable method for detecting different bacteria in foods (Bolton 1990;Coppola and FirstenbergEden 1988;Dupont et al 1994;Gibson et al 1992;Bullock and Frodsham 1989;Capell et al 1995;Ogden 1990). The present study confirmed these findings.…”

Section: Resultssupporting

confidence: 88%

ASSESSMENT OF IMPEDANCE MICROBIOLOGICAL METHOD FOR THE DETECTION OFESCHERICHIA COLIIN FOODS

Jawad

Marrow

Odumeru

1998

Rapid Methods Automation Mic

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This study was conducted to determine the sensitivity and specificity of the impedance‐based microbiological method for the detection of Escherichia coli in foods within 24 h of testing. A Malthus Microbiological Analyzer system (Malthus System V, Malthus Instruments Ltd., Bury, United Kingdom), and a modified Malthus Coliform Broth Medium (MCBM), and an incubation temperature of 44C were used. The sensitivity of the impedance method was determined by testing E. coli‐negative food samples spiked with different concentrations of E. coli. The specificity of the method was determined by testing E. coli‐negative food samples spiked with Klebsiella pneumoniae, Enterobacter cloacae and Pseudomonas aeruginosa. The test results were compared with those obtained by the Most Probable Number (MPN) method. Milk, milk products, raw and ready‐to‐eat meats, and vegetables were tested for the presence of E. coli by both methods. The sensitivity of the impedance method and the MPN method for the detection of foods containing 101 CFU/g was 100% and 84.4%, respectively. Both methods had a specificity of 100% for food samples spiked with 101 CFU/g E. coli. The specificity of the impedance and the MPN methods for the detection of E. coli in naturally contaminated milk and meat samples was 100% and 95.7% respectively. E. coli was detected in foods by the impedance method within 4–24 h of testing at a detection limit of 1 CFU/mL. These results demonstrate that the impedance method can be used as a rapid and sensitive method for the detection of E. coli in foods.

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Section: Resultssupporting

confidence: 88%

ASSESSMENT OF IMPEDANCE MICROBIOLOGICAL METHOD FOR THE DETECTION OFESCHERICHIA COLIIN FOODS

Jawad

Marrow

Odumeru

1998

Rapid Methods Automation Mic

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“…For quantitative studies with salmonellas and other microorganisms the impedimetric technique is now a well established rapid method (Zindulis 1984;Gibson 1987;Bullock and Frodsham 1989;Watson-Craik et al 1989;Bolton 1990;Smith et al 1991). Unlike the standard plate count technique, impedance microbiology monitors microbial metabolism in a real time modus.…”

Section: Introductionmentioning

confidence: 99%

The study of Salmonellaenteritidis growth kinetics using Rapid Automated Bacterial Impedance Technique

Fehlhaber

Krüger

1998

J Appl Microbiol

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In order to study strain-specific differences in their growth behaviour at different, and particularly lower, temperatures, generation times for 45 strains of Salmonella enteritidis isolated from food were determined impedimetrically over a temperature range from 7 to 42 degrees C. In practical terms, 7 degrees C is the minimum requirement for Salm. enteritidis growth, and generation time variability increases markedly as this temperature is reached. Reports in the literature describing psychrotrophic behaviour and multiplication at lower temperatures cannot be confirmed. Generation time variability increased as temperature moved away from the optimal range with variation coefficients tending to rise as temperature fell. The great variability of multiplication parameters near the growth limit found in Salm. enteritidis may also be a characteristic of other bacterial species. It is therefore imperative to commence studies on larger numbers of strains to allow prediction of their behaviour at lower temperatures.

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“…Recent advances in conductance/impedance tests for salmonellas have included media based on their ability to decarboxylate L-lysine (Arnott et af. 1988 ;Ogden 1988;Bullock & Frodsham 1989). Ogden (1988) showed that the total conductance change in Moeller's decarboxylase base increased with increasing amounts of lysine, and 132 of 134 known Salmonella isolates were detected in the final medium.…”

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confidence: 99%

Salmonella detection by a modified lysine conductance medium

Ogden¹

1990

Lett Appl Microbiol

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OGDEN, I.D. 1990 Salmonella detection by a modified lysine conductance medium. Letters in Applied Microbiology 11, 69--72.The statistical technique of response surface methodology was used to optimize the component concentrations of a Salmonella lysine medium for conductance responses on a Malthus microbial growth analyser. When the original and modified media were compared, conductance changes in pure cultures improved by 135 pS on average, and those from fopds both naturally contaminated and spiked with salmonellas by 220 pS. Salmonellas were successfully isolated from all assays after subculturing conductance broths onto xylose lysine desoxycholate agar and confirming by serology.

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Rapid impedance detection of salmonellas in confectionery using modified LICNR broth

Cited by 26 publications

References 7 publications

ASSESSMENT OF IMPEDANCE MICROBIOLOGICAL METHOD FOR THE DETECTION OFESCHERICHIA COLIIN FOODS

ASSESSMENT OF IMPEDANCE MICROBIOLOGICAL METHOD FOR THE DETECTION OFESCHERICHIA COLIIN FOODS

The study of Salmonellaenteritidis growth kinetics using Rapid Automated Bacterial Impedance Technique

Salmonella detection by a modified lysine conductance medium

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