Rapid in-situ detection kit (RisK): Development of loop-mediated isothermal amplification (LAMP) assay for the rapid identification of selected invasive alien fish in Malaysian freshwaters

Vythalingam, Lavanya Malini; Hossain, M. A. Motalib; Bhassu, Subha

doi:10.1016/j.mcp.2020.101683

Cited by 6 publications

(6 citation statements)

References 26 publications

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“…Colorimetric methods involves the use of different colorimetric reagents; the most commonly used reagent is calcein, which changes color according to the changes in magnesium ion concentrations (Pang et al, 2019). However, the color changes from orange (negative) to green (positive), which is not obvious and may lead to different assessments by different observers (Ali et al, 2017;Vythalingam et al, 2021). In this study, the pH indicator dye phenol red, included in the Colorimetric LAMP Master Mix (New England Biolabs), was used, as it is based on the decrease in pH caused by the hydrogen protons produced during LAMP (Nawattanapaiboon et al, 2021;.…”

Section: Discussionmentioning

confidence: 99%

Rapid identification of Takifugu genus using visual loop‐mediated isothermal amplification

Xie

et al. 2022

Journal of Food Science

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Some Takifugu species are commonly found in the coastal areas of China, Japan, Thailand, and Korea and cause pufferfish poisoning, which is toxic and even lethal to humans. From 2010 to 2015, there were 430 cases of pufferfish poisoning worldwide, resulting in 52 deaths. Identification of Takifugu species is imperative to reduce financial losses and ensure food safety. Here, visual loopmediated isothermal amplification (LAMP) was applied to identify Takifugu species. Conserved regions within the mitochondrial DNA among different Takifugu species were selected to design LAMP primers. In 55 min of amplification, sufficient DNA was obtained to observe the results with the naked eye, without the need for complicated instruments. The method was highly specific, with no cross-detection of 17 other fish species, namely, 7 Tetraodontiformes species and 10 commercially important fish. The method showed a detection limit of 0.1 ng Takifugu DNA and was successfully validated to detect Takifugu in cooked fish and the vomitus of poisoned patients. This rapid and visual LAMP method is a useful tool to prevent false labeling, protect consumer rights, and reduce the risk of pufferfish poisoning.

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Section: Discussionmentioning

confidence: 99%

Rapid identification of Takifugu genus using visual loop‐mediated isothermal amplification

Xie

et al. 2022

Journal of Food Science

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“…The most common application of DNA based detection in environmental biosecurity investigations is species identification (Linacre 2021). As a result, the use of LAMP to combat IWT extends to adulterated meat products (Cho et al 2014;Liu et al 2019;Nikunj and Vivek 2019;Sul et al 2019), detection of conservation significant species (But et al 2020;Wimbles et al 2021;Yu et al 2019), biosecurity screening (Blaser et al 2018b;Kyei-Poku et al 2020), invasive species detection in novel ecosystems (Rizzo et al 2021;Vythalingam et al 2021;Williams et al 2017), and disease monitoring (Sahoo et al 2016). Additionally, LAMP has a strong presence in bacterial and viral point-of-care detection methods research (Kashir and Yaqinuddin 2020;Nguyen et al 2019b).…”

Section: Lamp Research Applied To Environmental Biosecuritymentioning

confidence: 99%

“…Monitoring and related control programs have recently focussed on the role of eDNA in invasive species detection (Hunter et al 2015;Morisette et al 2021), with several studies focusing on LAMP as a potential eDNA monitoring tool (Vythalingam et al 2021;Williams et al 2017). The emphasis on monitoring primarily concerns invertebrate pests, as demonstrated by the development of LAMP assays as a means of point of entry detection or field-based detection.…”

Section: Invasive Species Monitoringmentioning

confidence: 99%

“…Additionally, the import and export of pets is often highly regulated or strictly banned under national jurisdictional law (Ege et al 2020). This has led to the development of detection methods for common aquatic pet species which double as invasive species in Malaysian waterways (Vythalingam et al 2021). The focus species included guppies, goldfish, siamese fighting fish, Amazon sailfin catfish, koi and African sharptooth catfish which were collected from pet shops and local aquariums for the purpose of LAMP development.…”

Section: Invasive Aquatic Species Detectionmentioning

confidence: 99%

“…Applications of LAMP extend to detection of human male DNA from sperm (Scott et al 2019) and differentiation of unknown body fluids, including venous blood, semen, and saliva, based on colorimetric responses (Jackson et al 2020;Layne et al 2021); with promising results for forensic science casework protocols. Similarly, the application of isothermal amplification methods for onsite monitoring of invasive species crossing transnational borders has been explored (Kyei-Poku et al 2020;Vythalingam et al 2021); as it offers an operational tool well suited for highly sensitive and specific field-based detection (Figure 1). LAMP shows great potential for compliance checks, species level presumptive detection, and situations lacking physical evidence, which rely on remnant trace DNA on surfaces or from tissue samples (Raele et al 2019).…”

Section: Introductionmentioning

confidence: 99%

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Shining a LAMP on the applications of isothermal amplification to monitoring illegal wildlife trade

Deliveyne¹,

Young²,

Austin³

et al. 2022

Preprint

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Environmental biosecurity risks associated with the Illegal Wildlife Trade (IWT) include the loss of biodiversity, threats to public health, and the proliferation of invasive alien species. To assist enforcement agencies in identifying trafficked species, rapid forensic techniques enable the detection of trace Environmental DNA (eDNA) where physical identification is not possible. Loop Mediated Isothermal Amplification (LAMP) is an emerging technique with recent applications in biosecurity and forensic sciences, and with potential to function as a field-based detection tool. Here we provide an overview of current research that applies LAMP to human and wildlife forensic science, including identification of ornamental wildlife parts, consumer products, and invasive species monitoring and biosecurity detection. We discuss the current scope of LAMP as applied to various wildlife crime scenarios and biosecurity checkpoint monitoring, highlight the specificity, sensitivity, and robustness for these applications, and review the potential utility of LAMP for rapid field-based detection within the IWT. Based on our assessment of the literature we recommend broader interest, research, and investment in LAMP as an appropriate field-based species detection method for a wide range of environmental biosecurity scenarios.

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Fish Traceability and Authenticity

GONZÁLEZ SOTELO,

GUNNLAUGSSON,

SCHRÖDER

et al. 2023

Current Challenges for the Aquatic Products Processing Industry

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Rapid in-situ detection kit (RisK): Development of loop-mediated isothermal amplification (LAMP) assay for the rapid identification of selected invasive alien fish in Malaysian freshwaters

Cited by 6 publications

References 26 publications

Rapid identification of Takifugu genus using visual loop‐mediated isothermal amplification

Rapid identification of Takifugu genus using visual loop‐mediated isothermal amplification

Shining a LAMP on the applications of isothermal amplification to monitoring illegal wildlife trade

Fish Traceability and Authenticity

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