Rapid in vitro multiplication of Melia azedarach L. (a multipurpose woody tree)

Abstract

An efficient regeneration protocol for rapid multiplication of Melia azedarach, an economically as well as medicinally important timber-yielding tree, was developed. Nearly 90% of the culture exhibited axillary bud sprouting and multiple shoot formation from nodal segments derived from 20-year-old candidate plus tree on Murashige and Skoog (MS) medium supplemented with 5 lM 6-benzyladenine (BA). The highest shoot regeneration frequency (92%), maximum number of multiple shoots (19.7 ± 0.31) as well as shoot length (4.9 ± 0.08 cm) was induced from nodal explants on MS medium amended with 5.0 lM BA, 0.5 lM indole-3-acetic acid (IAA) and 30 lM adenine sulfate (AdS). Addition of 250 mg l -1 ammonium sulphate, (NH 4 ) 2 SO 4 , and 100 mg l -1 K 2 SO 4 , prevented defoliation and tip burning without affecting the number of shoots. The explant harvest period also influenced the bud break and shoot sprouting from nodal segments. Repeated subculturing of nodal explants on fresh MS medium containing lower concentration of BA (2.5 lM) along with IAA (0.5 lM), AdS (30 lM) and additives was found most suitable growth regulator regime for achieving 1.2-fold increase in shoot multiplication rate. The percentage of shoot multiplication as well as the number of shoots per node remained the same during first three subculture passages, afterwards a decline was recorded. About 90% of the in vitro regenerated shoots were successfully rooted ex vitro by giving a pulse treatment of 250 lM indole-3-butyric acid for 15 min, followed by their transfer to thermocol cups containing soilrite. The raised plantlets were successfully acclimatized first under culture room conditions, then to green house with 85% survival rate. Keywords Meliaceae Á Bakain Á Micropropagation Á Axillary bud break Á Multiple shoots Á Ex vitro rooting Abbreviations AdS Adenine sulfate BA 6-Benzyladenine 2iP 2-Isopentenyladenine IAA Indole-3-acetic acid IBA Indole-3-butyric acid KIN Kinetin MS Murashige and Skoog medium NAA a-Naphthalene acetic acid Communicated by E. Lojkowska.

Rapid multiplication of Dalbergia sissoo Roxb.: a timber yielding tree legume through axillary shoot proliferation and ex vitro rooting

“…The addition of KCl and Ca(NO 3 ) 2 help in improving the growth of culture of woody legume. These findings are further supported by earlier reports (Rathore et al 2004;Husain and Anis 2009). According to Preece (1995), in vitro performance of explants can be improved by balancing the nutrient level in the medium.…”

The role of cytokinins on in vitro shoot production in Salix tetrasperma Roxb.: a tree of ecological importance

“…Means sharing the same letter are not significantly different (P = 0.05) using Duncan's multiple range test tried, BA was the most effective followed by Kn and 2 iP which is in accordance with earlier report on hybrid willow (S. matsudana 9 alba NZ-1002) by Bhojwani (1980). The effectiveness of BA on multiple shoot bud differentiation has also been demonstrated in a number of cases using a variety of explants Husain and Anis 2009;Siddique and Anis 2009). The differentiation of shoot buds was confirmed by histological examination of cultured explants.…”

In vitro plant regeneration of Albizia lebbeck (L.) from seed explants

“…It has been reported that ex vitro-rooted plants are better suited to tolerate environmental stresses (Phulwaria et al, 2011). Rooting under ex vitro conditions has been successfully utilized for the establishment of several woody plants including Tectona grandis (Tiwari et al, 2002), Vitex nigundo (Ahmad and Anis, 2007), Melia azedarach (Husain and Anis, 2009) and Tecomella undulata (Varshney and Anis, 2012). Therefore, auxin dipped ex-vitro rooting is the most effective method for root induction in the present investigation.…”