The role of cytokinins on in vitro shoot production in Salix tetrasperma Roxb.: a tree of ecological importance

Abstract

A valuable tropical tree, Salix tetrasperma Roxb. commonly known as Indian willow has been investigated for its in vitro regeneration potential using nodal explants obtained from a 30-year-old elite tree. Agarsolidified Woody Plant Medium (WPM) containing different concentrations of Plant Growth Regulators (PGRs) was used in the study. Shoot induction response was best on WPM supplemented with 6-benzyladenine (5.0 lM) where 90% explants responded with an average shoot number (4.40 ± 0.50) and shoot length (0.92 ± 0.04) after 6 weeks of culture. However, multiplication and elongation was best recorded when BA (5.0 lM) treated shoot clusters were transferred to WPM containing BA (1.0 lM) ? NAA (0.5 lM) where 18.40 ± 0.92 well-grown healthy shoots with an average shoot length of 5.30 ± 0.43 cm were obtained on completion of 12 weeks culture period. In vitro rooting of shoots was best achieved in half-strength WPM containing 0.5 lM IBA. Well-rooted plantlets were successfully hardened off and acclimatized in plastic cups containing sterile Soilrite. These plantlets were then transferred to pots containing normal garden soil followed by transfer to greenhouse and ultimately to field under full sun.

RETRACTED ARTICLE: Influencing micropropagation in Clitoria ternatea L. through the manipulation of TDZ levels and use of different explant types

“…About 88 % plants survived the hardening procedure without any detectable morphological variations. Such process of slow hardening and acclimatization of tissue culture raised plantlets have been found suitable with many micropropagation protocols (Ahmad and Anis 2007;Khan et al 2011).…”

Management of cytokinin–auxin interactions for in vitro shoot proliferation of Althaea officinalis L.: a valuable medicinal plant

“…Most shoot buds exposed no visible connection with the original vascular tissue, although they appeared to have originated from the meristematic zone beneath the epidermis. It is possible that the initial shoots developed from pre-existing meristems (Khan et al 2011;Brondani et al 2012). These results showed consistency with the findings of Ahmad and Anis (2007), Paunescu (2010) and Fatima and Anis (2012).…”

Effect of copper and zinc on the in vitro regeneration of Rauvolfia serpentina

“…The effectiveness of IBA for efficient in vitro rooting the regenerants have been reported by Faisal et al (2005) in Rauvolfia tetraphylla, Khan et al (2011) in Salix tetrasperma, and also by Ahmad and Anis (2011) in Vitex negundo. Successful hardening and acclimatization of the regenerated plantlets was achieved first on Soilrite followed by transfer to normal garden soil, a procedure found suitable for many micropropagation protocols.…”