Rapid in vitro propagation of cauliflower

Kumar, Atul; Kumar, Vikas; Kumar, Jitendra

doi:10.1016/0168-9452(93)90237-t

Cited by 31 publications

(36 citation statements)

References 7 publications

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“…Light is one of the important environmental factors that controls plant growth, development, morphogenesis, metabolism and chlorophyll content in plant cell, tissue and organ cultures (Dou et al 2017). Light intensity and light source are important parameters that influence shoot regeneration, fresh weight, and secondary metabolite biosynthesis during micropropagation in a variety of crops, including Persian shallot (Farhadi et al 2017), cauliflower (Kumar et al 1993), date palm (Meziani et al 2015) and Cistanche (Ouyang et al 2003). Light can also influence the efficacy of plant growth regulators (PGRs) as well as adjustment of endogenous hormone levels.…”

Section: Introductionmentioning

confidence: 99%

Effects of light intensity and plant growth regulators on callus proliferation and shoot regeneration in the ornamental succulent Haworthia

et al. 2019

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Background Haworthia are desert succulents belonging to the Asphodelaceae family. Haworthia species are cultivated commercially as ornamentals and some rare species are quite valuable at retail market but growth slowly and difficult to propagation. However, an efficient micropropagation protocol was remained insufficient. Results The organogenic cultures obtained from inflorescence explants were cultured on Murashige and Skoog (MS) medium supplemented with various combinations of 6-benzylaminopurine (BA) and α-naphthalene acetic acid (NAA) under a light intensity of 10 μmol m −2 s −1 or 45 μmol m −2 s −1 . The highest callus proliferation index (93.15%) with 1.0 mg L −1 BA + 0.1 mg L −1 NAA under a light intensity of 10 μmol m −2 s −1 . The best shoot proliferation rates were on media with either 1 mg L −1 BA + 0–0.4 mg L −1 NAA (65.57–81.01%) under a light intensity of 45 μmol m −2 s −1 . The highest root length (15.57 mm) and the highest rooting frequency (17 roots per shoot) were obtained when adventitious shoots were inoculated on MS medium with 0.4 mg L −1 NAA + 0.4 mg L −1 IBA. The survival rate of the transplanted plantlets was about 100%. The efficient micropropagation protocol proliferated Haworthia regenerate plants from inflorescence within 11 weeks. Conclusions The present study determined the best combination of light intensity and plant growth regulators (PGRs) for improved organogenesis of Haworthia during propagation by tissue culture. This optimized protocol showed light intensity is an important factor for efficient callus or shoot regeneration. These results indicate that it will be useful to optimize the light conditions for future commercial cultivation, germplasm conservation, genetic engineering and molecular biology research of this ornamental plant.

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Section: Introductionmentioning

confidence: 99%

Effects of light intensity and plant growth regulators on callus proliferation and shoot regeneration in the ornamental succulent Haworthia

et al. 2019

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“…The outermost layer of cauliflower curd is made of several million meristems (Kieffer et al 2001) and the use of curd meristematic tissue for in vitro culture has been investigated for micropropagation and the production of virus free plants (Grout and Crisp 1977;Kumar et al 1993). The use of the meristematic layer of cauliflower curd is considered to be superior compared with the use of conventional protocols using seedling or leaf explants which have been found to be labour intensive (Pow 1969;Kumar et al 1993).…”

Section: Introductionmentioning

confidence: 98%

“…The use of the meristematic layer of cauliflower curd is considered to be superior compared with the use of conventional protocols using seedling or leaf explants which have been found to be labour intensive (Pow 1969;Kumar et al 1993). Kieffer et al (2001) designed an effective protocol for the production of cauliflower propagules from fractionated and graded curd and these propagules were suggested to be suitable for encapsulation in sodium alginate matrices for the production of artificial seeds.…”

Section: Introductionmentioning

confidence: 99%

Encapsulation of cauliflower (Brassica oleracea var botrytis) microshoots as artificial seeds and their conversion and growth in commercial substrates

Rihan

Al-Issawi

Burchett

et al. 2011

Plant Cell Tiss Organ Cult

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An effective protocol for the mass production of cauliflower microshoots was refined using the meristematic layer of cauliflower curd. After the meristematic layer was surface sterilized and shaved off, a commercial blender was used for homogenization and several blending treatments were tested in the range 15-120 s and 30 s was found to be optimal in terms of the amount explants produced and their subsequent growth ability. Explants were cultivated in S23 liquid medium (4.4 g L -1 MS (Murashige and Skoog) and 3% v/w sucrose) supplemented with several combinations of plant growth regulators (PGRs) including 1 and 2 mg L -1 of Kinetin in combination with three types of auxins (indole butyric acid (IBA), Naphthaleneacetic acid (NAA) and Indole-3-acetic acid (IAA)), each at 1 and 2 mg L -1 concentration. The use of 2 mg L -1 Kinetin and 1 mg L -1 IBA gave the best results in terms of its effects on explant induction. Microshoots of different sizes were encapsulated in a sodium alginate matrix and the optimal stage suitable for the production of artificial seeds was assessed in terms of both subsequent conversion and plantlet viability. The feasibility of cultivating cauliflower artificial seeds in commercial substrates (compost, vermiculite, perlite and sand) irrigated with different solution mixtures including sterilized distilled water (SDW), PGRs-free S23 medium and S23 medium supplemented with Kinetin (1 and 2 mg L -1 ) and IBA or NAA at (1 and 2 mg L -1 ) was investigated. The use of 2 mg L -1 Kinetin and 2 mg L -1 NAA applied with S23 gave the optimal response with both perlite and compost.This study showed high growth capacity of cauliflower artificial seeds in commercial substrates which is considered a promising step for their direct use in vivo.

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“…In vitro propagation of plants can be achieved through different techniques (Kumar and Reddy 2011). Somatic embryogenesis and organogenesis are two different techniques for in vitro regeneration of plants (Phillips 2004).…”

Section: Introductionmentioning

confidence: 99%

Nuclear Magnetic Resonance Characterizes Metabolic Differences in Cymbopogon Schoenanthus Subsp. Proximus Embryogenic and Organogenic Calli and their Regenerated Shoots

Abdelsalam

Chowdhury

Boroujerdi

et al. 2021

Preprint

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Cymbopogon schoenanthus subsp. proximus is a threatened wild grass well known for its folk medicine uses and possesses a broad spectrum of pharmacological properties. In this study, the metabolite differences between embryogenic and organogenic calli have been characterized. Changes in the metabolome of somatic embryogenesis and organogenesis have been studied by comparing the metabolites of morphogenic calli with their respective regenerated shoots. Based on 1H and HSQC NMR data of the polar extracts, a total of 51 metabolites have been identified. Statistical analyses were performed to visualize the pattern of metabolites distribution among the studied groups. Glycolate present in organogenic calli, but not in organogenic calli. Some metabolites like glucose-6-phosphate, 4-aminobutyrate and arginine showed elevated concentrations in embryogenic calli than organogenic calli. While sucrose and myo-inositol up regulated in organogenic calli than embryogenic calli. Metabolites that marked embryogenic shoots from embryogenic calli have been described. Quantitatively, embryogenic shoots showed higher concentrations of sucrose, some amino acids, and trigonelline compared with embryogenic calli. In contrast, glucose, fructose, and arginine accumulated in embryogenic calli more than in embryogenic shoots. Likewise, metabolites characterized organogenic shoots from organogenic calli have been identified. Sucrose, several amino acids and trigonelline were detected in higher concentrations in organogenic shoots than in organogenic calli while monosaccharides and arginine accumulated in organogenic calli than in organogenic shoots. This study represents a first step towards understanding the metabolic activity of calli and shoots regenerated through different morphogenetic pathways.

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Rapid in vitro propagation of cauliflower

Cited by 31 publications

References 7 publications

Effects of light intensity and plant growth regulators on callus proliferation and shoot regeneration in the ornamental succulent Haworthia

Effects of light intensity and plant growth regulators on callus proliferation and shoot regeneration in the ornamental succulent Haworthia

Encapsulation of cauliflower (Brassica oleracea var botrytis) microshoots as artificial seeds and their conversion and growth in commercial substrates

Nuclear Magnetic Resonance Characterizes Metabolic Differences in Cymbopogon Schoenanthus Subsp. Proximus Embryogenic and Organogenic Calli and their Regenerated Shoots

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