Rapid, inexpensive fabrication of electrophoretic microdevices for fluorescence detection

Nelson, Daniel A.; Thompson, Brandon L.; Tsuei, An‐Chi; Nouwairi, Renna; Birch, Christopher; DuVall, Jacquelyn A.; Roux, Delphine Le; Li, Jingyi; Root, Brian E.; Landers, James P.

doi:10.1002/elps.202200090

Cited by 3 publications

(2 citation statements)

References 23 publications

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“…Moreover, separation channel designs and centrifugation conditions were improved by Nelson et al. [57], which enabled successful polymer loading within less than 3 min. Additionally, DNA fragments of 147 and 167 bases were separated and sized by the devices as 148.62 ± 2 and 166.48 ± 3 bases, respectively [57].…”

Section: In Forensic Geneticsmentioning

confidence: 99%

Application of microfluidic technologies in forensic analysis

Guo,

Zhang,

Zhang

et al. 2023

Electrophoresis

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The application of microfluidic technology in forensic medicine has steadily expanded over the last two decades due to the favorable features of low cost, rapidity, high throughput, user‐friendliness, contamination‐free, and minimum sample and reagent consumption. In this context, bibliometric methods were adopted to visualize the literature information contained in the Science Citation Index Expanded from 1989 to 2022, focusing on the co‐occurrence analysis of forensic and microfluidic topics. A deep interpretation of the literature was conducted based on co‐occurrence results, in which microfluidic technologies and their applications in forensic medicine, particularly forensic genetics, were elaborated. The purpose of this review is to provide an impartial evaluation of the utilization of microfluidic technology in forensic medicine. Additionally, the challenges and future trends of implementing microfluidic technology in forensic genetics are also addressed.

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Section: In Forensic Geneticsmentioning

confidence: 99%

Application of microfluidic technologies in forensic analysis

Guo,

Zhang,

Zhang

et al. 2023

Electrophoresis

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“…However, limitations in adopting microfluidic systems often stem from failing to address the costly nature of the microfluidic consumable [ 31 ]. Our lab has defined a cost-effective method for rapid, iterative prototyping of microfluidic CDs (print-cut-laminate technique; PCL [ 32 ]) with complex, intricate architectures for chemical and biochemical assays ranging from DNA purification and genome analysis to illicit drug detection and explosives sensing [ 33 , 34 , 35 ].…”

Section: Introductionmentioning

confidence: 99%

Rapid Microchip Electrophoretic Separation of Novel Transcriptomic Body Fluid Markers for Forensic Fluid Profiling

et al. 2022

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Initial screening of criminal evidence often involves serological testing of stains of unknown composition and/or origin discovered at a crime scene to determine the tissue of origin. This testing is presumptive but critical for contextualizing the scene. Here, we describe a microfluidic approach for body fluid profiling via fluorescent electrophoretic separation of a published mRNA panel that provides unparalleled specificity and sensitivity. This centrifugal microfluidic approach expedites and automates the electrophoresis process by allowing for simple, rotationally driven flow and polymer loading through a 5 cm separation channel; with each disc containing three identical domains, multi-sample analysis is possible with a single disc and multi-sample detection per disc. The centrifugal platform enables a series of sequential unit operations (metering, mixing, aliquoting, heating, storage) to execute automated electrophoretic separation. Results show on-disc fluorescent detection and sizing of amplicons to perform comparably with a commercial ‘gold standard’ benchtop instrument and permitted sensitive, empirical discrimination between five distinct body fluids in less than 10 min. Notably, our microfluidic platform represents a faster, simpler method for separation of a transcriptomic panel to be used for forensically relevant body fluid identification.

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Rapid, inexpensive fabrication of electrophoretic microdevices for fluorescence detection

et al. 2022

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The laser print, cut, and laminate (PCL) method for microfluidic device fabrication can be leveraged for rapid and inexpensive prototyping of electrophoretic microchips useful for optimizing separation conditions. The rapid prototyping capability allows the evaluation of fluidic architecture, applied fields, reagent concentrations, and sieving matrix, all within the context of using fluorescencecompatible substrates. Cyclic olefin copolymer and toner-coated polyethylene terephthalate (tPeT) were utilized with the PCL technique and bonding methods optimized to improve device durability during electrophoresis. A series of separation channel designs and centrifugation conditions that provided successful loading of sieving polymer in less than 3 min was described. Separation of a 400-base DNA sizing ladder provided calculated base resolution between 3 and 4 bases, a greater than 18-fold improvement over separations on similar substrates. Finally, the accuracy and precision capabilities of these devices were demonstrated by separating and sizing DNA fragments of 147 and 167 bases as 148.62 ± 2 and 166.48 ± 3 bases, respectively.

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Rapid, inexpensive fabrication of electrophoretic microdevices for fluorescence detection

Cited by 3 publications

References 23 publications

Application of microfluidic technologies in forensic analysis

Application of microfluidic technologies in forensic analysis

Rapid Microchip Electrophoretic Separation of Novel Transcriptomic Body Fluid Markers for Forensic Fluid Profiling

Rapid, inexpensive fabrication of electrophoretic microdevices for fluorescence detection

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