Rapid isolation of cancer cells using microfluidic deterministic lateral displacement structure

Liu, Zongbin; Huang, Fei; Du, Jinghui; Wang, Shu; Feng, Hongtao; Xu, Xiaoping; Chen, Yan

doi:10.1063/1.4774308

Cited by 191 publications

(128 citation statements)

References 31 publications

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“…Larger size cells will move laterally toward to the side of channel; smaller particles will flow along the original trajectory. [61][62][63][64][65] DLD arrays allow continuous cancer cell enrichment from peripheral blood. However, the cancer cell purity was still very low after enrichment.…”

Section: Hydrodynamicsmentioning

confidence: 99%

Microfluidics cell sample preparation for analysis: Advances in efficient cell enrichment and precise single cell capture

et al. 2017

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Single cell analysis has received increasing attention recently in both academia and clinics, and there is an urgent need for effective upstream cell sample preparation. Two extremely challenging tasks in cell sample preparation-highefficiency cell enrichment and precise single cell capture-have now entered into an era full of exciting technological advances, which are mostly enabled by microfluidics. In this review, we summarize the category of technologies that provide new solutions and creative insights into the two tasks of cell manipulation, with a focus on the latest development in the recent five years by highlighting the representative works. By doing so, we aim both to outline the framework and to showcase example applications of each task. In most cases for cell enrichment, we take circulating tumor cells (CTCs) as the target cells because of their research and clinical importance in cancer. For single cell capture, we review related technologies for many kinds of target cells because the technologies are supposed to be more universal to all cells rather than CTCs. Most of the mentioned technologies can be used for both cell enrichment and precise single cell capture. Each technology has its own advantages and specific challenges, which provide opportunities for researchers in their own area. Overall, these technologies have shown great promise and now evolve into real clinical applications. Published by AIP Publishing. [http://dx

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Section: Hydrodynamicsmentioning

confidence: 99%

Microfluidics cell sample preparation for analysis: Advances in efficient cell enrichment and precise single cell capture

et al. 2017

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“…Recently, there have been many attempts to develop a reliable, rapid and sensitive method for the isolation and detection of CTCs, including immunomagnetic separation, 2,3 affinity chromatography separation, 4,5 and label-free separation. [6][7][8][9][10][11][12] Despite the detection and analysis of CTCs offer a promising non-invasive diagnostic approach for the diagnosis and prognosis of cancer, its use in clinical practice remains limited because of the rarity of CTCs in blood, the difficulty of isolating CTCs and the complexity of CTCs identification and enumeration. In contrast to the isolation and detection of CTCs, the protein-based biomarker tests are easier and more convenient which do not need labor-intensive cell capture and identification and has the potential to allow rapid point-of-care use.…”

Section: Introductionmentioning

confidence: 99%

Direct detection of cancer biomarkers in blood using a “place n play” modular polydimethylsiloxane pump

Zhang

Liao

et al. 2013

Biomicrofluidics

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Cancer biomarkers have significant potential as reliable tools for the early detection of the disease and for monitoring its recurrence. However, most current methods for biomarker detection have technical difficulties (such as sample preparation and specific detector requirements) which limit their application in point of care diagnostics. We developed an extremely simple, power-free microfluidic system for direct detection of cancer biomarkers in microliter volumes of whole blood. CEA and CYFRA21-1 were chosen as model cancer biomarkers. The system automatically extracted blood plasma from less than 3 ll of whole blood and performed a multiplex sample-to-answer assay (nano-ELISA (enzyme-linked immunosorbent assay) technique) without the use of external power or extra components. By taking advantage of the nano-ELISA technique, this microfluidic system detected CEA at a concentration of 50 pg/ml and CYFRA21-1 at a concentration of 60 pg/ml within 60 min. The combination of PnP polydimethylsiloxane (PDMS) pump and nano-ELISA technique in a single microchip system shows great promise for the detection of cancer biomarkers in a drop of blood. V C 2013 AIP Publishing LLC. [http://dx

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“…29 Highly efficient capture of CTCs was reported by using nanostructured silicon substrates with integrated chaotic micromixers. 30 Label-free cancer cell separation techniques, such as the size-based separation using deterministic lateral displacement structure 31 or using dielectrophoretic techniques, 32 were also reported.…”

Section: Introductionmentioning

confidence: 99%

Isolation of viable cancer cells in antibody-functionalized microfluidic devices

et al. 2014

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Microfluidic devices functionalized with EpCAM antibodies were utilized for the capture of target cancer cells representing circulating tumor cells (CTCs). The fraction of cancer cells captured from homogeneous suspensions is mainly a function of flow shear rate, and can be described by an exponential function. A characteristic shear rate emerges as the most dominant parameter affecting the cell attachment ratio. Utilizing this characteristic shear rate as a scaling factor, all attachment ratio results for various combinations of receptor and ligand densities collapsed onto a single curve described by the empirical formula. The characteristic shear rate increases with both cell-receptor and surface-ligand densities, and empirical formulae featuring a product of two independent cumulative distributions described well these relationships. The minimum detection limit in isolation of target cancer cells from binary mixtures was experimentally explored utilizing microchannel arrays that allow high-throughput processing of suspensions about 0.5 ml in volume, which are clinically relevant, within a short time. Under a twostep attachment/detachment flow rate, both high sensitivity (almost 1.0) and high specificity (about 0.985) can be achieved in isolating target cancer cells from binary mixtures even for the lowest target/non-target cell concentration ratio of 1:100 000; this is a realistic ratio between CTCs and white blood cells in blood of cancer patients. Detection of CTCs from blood samples was also demonstrated using whole blood from healthy donors spiked with cancer cells. Finally, the viability of target cancer cells released after capture was confirmed by observing continuous cell growth in culture. V C 2014 AIP Publishing LLC.

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Rapid isolation of cancer cells using microfluidic deterministic lateral displacement structure

Cited by 191 publications

References 31 publications

Microfluidics cell sample preparation for analysis: Advances in efficient cell enrichment and precise single cell capture

Microfluidics cell sample preparation for analysis: Advances in efficient cell enrichment and precise single cell capture

Direct detection of cancer biomarkers in blood using a “place n play” modular polydimethylsiloxane pump

Isolation of viable cancer cells in antibody-functionalized microfluidic devices

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