Rapid LC-UV Analysis of Iohexol in Canine Plasma for Glomerular Filtration Rate Determination

Pöytäkangas, Merja; Saario-Paunio, Elise; Putkonen, T; Saastamoinen, Ilkka; Frías, Rafael; Spillmann, Thomas; Saloniemi, Hannu

doi:10.1365/s10337-009-1445-9

Cited by 5 publications

(10 citation statements)

References 18 publications

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“…A mobile phase of 40:60 v/v 50 mM phosphate buffer pH 5: methanol showed the most satisfactory results in consideration of peak shape, system stability, separation efficiency, and time. Separation of iohexol stereoisomers was also achieved as reported for RP particle based columns , a major peak with more than 95% of iohexol was observed (Fig. A).…”

Section: Resultssupporting

confidence: 63%

“…Three types of monoliths, SMA‐EDMA, PEDAS‐EDMA, and MAA‐EDMA were synthesized and preliminarily investigated. Although successful separation of iohexol on reversed‐phase particle based columns has been reported , no retention of iohexol on the reversed‐phased SMA‐EDMA monolith containing C 18 functional groups was obtained. Similar result was observed from a mixed mode monolith containing both polar –OH and nonpolar –C 17 functional groups; PEDAS‐EDMA monolith.…”

Section: Resultsmentioning

confidence: 99%

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Simple and fast analysis of iohexol in human serums using micro‐hydrophilic interaction liquid chromatography with monolithic column

Chaloemsuwiwattanakan

Sangcakul

Kitiyakara

et al. 2016

J of Separation Science

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A simple and rapid method based on micro-liquid chromatography using a synthetic monolithic capillary column was developed for determination of iohexol in human serums, a marker to evaluate the glomerular filtration rate. A hydrophilic methacrylic acid-ethylene dimethacrylate monolith provided excellent selectivity and efficiency for iohexol with separation time of 3 min using a mobile phase of 40:60 v/v 50 mM phosphate buffer pH 5/methanol. Four serum protein removal, methods using perchloric acid, 50% acetonitrile, 0.1 M zinc sulfate, and centrifuge membrane filter were examined. The method of zinc sulfate was chosen due to its simplicity, compatibility with the mobile phase system, nontoxicity, and low cost. Interday calibration curves were conducted over iohexol concentrations range of 2-500 mg/L (R(2) = 0.9997 ± 0.0001) with detection limit of 0.44 mg/L. Intra- and interday precisions for peak area and retention time were less than 2.8 and 1.4%, respectively. The method was successfully applied to serum samples with percent recoveries from 102 to 104. The method was applied to monitor released iohexol from healthy subject. Compared with the commercially available reversed-phase high-performance liquid chromatography method, the presented method provided simpler chromatogram, faster separation with higher separation efficiency and much lower sample and solvent consumption.

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Section: Resultssupporting

confidence: 63%

Section: Resultsmentioning

confidence: 99%

Simple and fast analysis of iohexol in human serums using micro‐hydrophilic interaction liquid chromatography with monolithic column

Chaloemsuwiwattanakan

Sangcakul

Kitiyakara

et al. 2016

J of Separation Science

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“…This value is low enough to evaluate concentrations until 270 min after injection of 1,500 mg of Omnipaque®. Moreover, accuracy and precision are also in the same ranges as previously described .…”

Section: Discussionmentioning

confidence: 92%

“…Few HPLC methods have been described for iohexol quantification (16,21,(28)(29)(30)(31). We developed a rapid (run time <10 min) HPLC method validated according to the recommendations of ICH guidelines, compatible with those described in the literature.…”

Section: Discussionmentioning

confidence: 99%

“…Many studies showed a high correlation between plasma clearance of iohexol, a nonionic molecule commonly used as X-ray contrast media agent, and other exogenous GFR markers such as DTPA (diethylenetriaminepentaacetic acid), radiolabeled EDTA (ethylenediaminetetraacetic acid), and inulin, in adult patients with normal or impaired renal function (16)(17)(18)(19)(20)(21). Several analytical methods have been described to measure plasma iohexol concentrations such as X-ray fluorescence (22,23), mass spectrometry (24,25), and chromatographic methods (16,21,(26)(27)(28)(29)(30)(31).…”

Section: Introductionmentioning

confidence: 99%

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Routine Determination of GFR in Renal Transplant Recipients by HPLC Quantification of Plasma Iohexol Concentrations and Comparison With Estimated GFR

Castagnet¹,

Blasco

Vourc’h

et al. 2012

Clinical Laboratory Analysis

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Estimated glomerular filtration rate (eGFR) methods are not sufficiently reliable in renal transplant recipients (RTR) and should be replaced by iohexol plasma clearance measurement. However, this method has poor availability in health centers. The aim of our study was to develop a high-performance liquid chromatography (HPLC) method for plasma iohexol measurement in routine practice and to evaluate its plasma clearance as a reference of GFR. We developed an HPLC method using UV detection. We evaluated sample storage conditions to provide recommendations for routine practice. Then, we compared GFRbased on plasma iohexol clearance (GFR-iohexol) to eGFR using modification of diet in renal disease, Cockcroft and Gault, and CDK-EPIequations in 40 RTR. The method was validated over a concentration range of 15-300 μg/l. Excellent linearity (r > 0.998), inter- and intraday precision (CV < 3.3%), and accuracy (>96.8%) were complied with ICH guidelines. We also demonstrated excellent samples stability (9 days). Although eGFR methods are not references in RTR, we found a correct concordance between eGFR and GFR-iohexol in our population. To conclude, our method is simple, rapid, accurate, and reliable for routine clinical and research use especially in RTR.

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Quantitative determination of exo- and endo-iohexol in canine and feline samples using high performance liquid chromatography with ultraviolet detection

Baere

Smets

Finch

et al. 2012

Journal of Pharmaceutical and Biomedical Analysis

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Rapid LC-UV Analysis of Iohexol in Canine Plasma for Glomerular Filtration Rate Determination

Cited by 5 publications

References 18 publications

Simple and fast analysis of iohexol in human serums using micro‐hydrophilic interaction liquid chromatography with monolithic column

Simple and fast analysis of iohexol in human serums using micro‐hydrophilic interaction liquid chromatography with monolithic column

Routine Determination of GFR in Renal Transplant Recipients by HPLC Quantification of Plasma Iohexol Concentrations and Comparison With Estimated GFR

Quantitative determination of exo- and endo-iohexol in canine and feline samples using high performance liquid chromatography with ultraviolet detection

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