Rapid location of Glomerella leaf spot resistance gene locus in apple by whole genome re-sequencing

Liu, Yuanxia; Lan, Jinhao; Li, Qing; Zhang, Yugang; Wang, Caihong; Hirata, Dai

doi:10.1007/s11032-017-0684-y

Cited by 9 publications

(7 citation statements)

References 38 publications

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“…Symptoms of the lenticel-infecting fungi usually do not appear until the fruit has been kept in cold storage for some weeks and thereafter at room temperature for some days. Moreover, storage-rot-producing species of Neofabraea can also cause perennial cankers (anthracnose) on apple trees, thus ensuring year-round survival of the pathogen in the orchard [ 46 ], while species of Colletotrichum are responsible for the widely dispersed Glomerella leaf spot disease (named after the sexual stage of C. gloeosporioides , namely Glomerella cingulata ) [ 35 , 36 ].…”

Section: Fungi That Cause Storage Rotmentioning

confidence: 99%

“…Nowadays, genes can also be located using the increasingly affordable methods for whole-genome re-sequencing. Together with bulked segregant analysis, this type of data was used to locate genes for resistance against Glomerella leaf spot caused by C. fructicola [ 35 , 36 ]. Genome-wide comparison of SNP profiles between the resistant and the susceptible bulks, constructed from F1 individuals derived from a cross between ‘Golden Delicious’ and ‘Fuji’, enabled the fine-mapping of an Rgls locus on LG15.…”

Section: Resistance Mechanisms and Quest For Resistance Genesmentioning

confidence: 99%

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Review of the Impact of Apple Fruit Ripening, Texture and Chemical Contents on Genetically Determined Susceptibility to Storage Rots

Nybom

Ahmadi-Afzadi

Rumpunen

et al. 2020

Plants

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Fungal storage rots like blue mould, grey mould, bull’s eye rot, bitter rot and brown rot destroy large amounts of the harvested apple crop around the world. Application of fungicides is nowadays severely restricted in many countries and production systems, and these problems are therefore likely to increase. Considerable variation among apple cultivars in resistance/susceptibility has been reported, suggesting that efficient defence mechanisms can be selected for and used in plant breeding. These are, however, likely to vary between pathogens, since some fungi are mainly wound-mediated while others attack through lenticels or by infecting blossoms. Since mature fruits are considerably more susceptible than immature fruits, mechanisms involving fruit-ripening processes are likely to play an important role. Significant associations have been detected between the susceptibility to rots in harvested fruit and various fruit maturation-related traits like ripening time, fruit firmness at harvest and rate of fruit softening during storage, as well as fruit biochemical contents like acidity, sugars and polyphenols. Some sources of resistance to blue mould have been described, but more research is needed on the development of spore inoculation methods that produce reproducible data and can be used for large screenings, especially for lenticel-infecting fungi.

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Section: Fungi That Cause Storage Rotmentioning

confidence: 99%

Section: Resistance Mechanisms and Quest For Resistance Genesmentioning

confidence: 99%

Review of the Impact of Apple Fruit Ripening, Texture and Chemical Contents on Genetically Determined Susceptibility to Storage Rots

Nybom

Ahmadi-Afzadi

Rumpunen

et al. 2020

Plants

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“…The techniques were successfully used in research on the apple resistance to Glomerella leaf spot and have made possible to determine the SNPs saturating the quantitative trait locus (QTL) fragments responsible for the trait, which, according to current knowledge, was considered to be regulated by a recessive allele at a single gene locus. In such approaches, the resequencing technique has a great impact on the determination of specific genome fragments that control quantitative traits (Liu et al 2017).…”

Section: Single Nucleotide Polymorphism and Apple Genotypingmentioning

confidence: 99%

Structural and functional apple genomics and its application in breeding

Keller-Przybyłkowicz¹

2018

CZECH J GENET PLANT

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The present paper summarized some knowledge of modern technologies, applied in apple (Malus domestica) genome studies. New generation sequencing allowed single nucleotide polymorphism (SNP) chip technologies for genotyping, description of functional apple genes, characterization of the evolutionary results in apple genome fragment transition, as well as phylogenetic reconstruction of the genus Malus, being the confirmed progeny of M. sieviersii. Based on these technologies, newly developed putative markers may give the most important biological data such as age, geographical origins, tissue type determination, and external visible characters. The new generation genotyping platforms, representing very high efficiency, are now successfully applied for random apple genome-wide association (GWA) studies as well pedigree-based analysis and marker-assisted selection (MAS).

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“…Glomerella leaf spot (GLS) is a severe apple disease caused by Colletotrichum fructicola , which has affected the industrial development of apple ( Malus domestica ) in the last decades [ 1 , 2 ]. GLS mainly occurred in some major apple cultivars, such as ‘Gala’ and ‘Golden Delicious’.…”

Section: Introductionmentioning

confidence: 99%

A TIR-NBS-LRR Gene MdTNL1 Regulates Resistance to Glomerella Leaf Spot in Apple

Liu

Bai

et al. 2022

IJMS

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Glomerella leaf spot (GLS), caused by the fungus Colletotrichum fructicola, is one of the most devastating apple diseases. Our previous study reported that the GLS resistance locus was defined on the chromosome 15 region. Here, we further found a single-nucleotide polymorphism (SNP) site (SNP7309212) in the GLS resistance that was able to distinguish resistant cultivars (lines) from susceptible ones. On the basis of the SNP site, we cloned a TNL gene from the GLS resistant locus and named it MdTNL1 (NCBI Accession Number: ON402514). This gene contains a toll/interleukin-1 receptor transmembrane domain (TIR), nucleotide-binding sites (NBS), and leucine-rich repeat (LRR) domain. Subcellular location indicated that MdTNL1 was expressed in the nucleus and cell membrane. Ectopic overexpression of MdTNL1 in Nicotiana benthamiana caused cell death. We further demonstrated allelic polymorphisms in MdTNL1. It is noteworthy that NBS and LRR domains of the MdTNL1 protein serve as the repository for generating allelic diversity. Quantitative real-time PCR (qRT-PCR) assay revealed that MdTNL1 was highly expressed in resistant apple cultivar ‘Fuji’ after inoculation with C. fructicola, whereas susceptible cultivar ‘Golden Delicious’ exhibited low expression after inoculation. Over-expression of MdTNL1-1 in susceptible apple fruits and leaves improved disease resistance, while in ‘Orin’ calli, silencing the MdTNL1-1 gene conversely decreased GLS resistance. In conclusion, we identified a GLS associated with SNP7309212 and demonstrated that a TIR-NBS-LRR gene MdTNL1-1 positively regulates GLS resistance in apple.

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Rapid location of Glomerella leaf spot resistance gene locus in apple by whole genome re-sequencing

Cited by 9 publications

References 38 publications

Review of the Impact of Apple Fruit Ripening, Texture and Chemical Contents on Genetically Determined Susceptibility to Storage Rots

Review of the Impact of Apple Fruit Ripening, Texture and Chemical Contents on Genetically Determined Susceptibility to Storage Rots

Structural and functional apple genomics and its application in breeding

A TIR-NBS-LRR Gene MdTNL1 Regulates Resistance to Glomerella Leaf Spot in Apple

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