Rapid Measurement of Blood Propofol Levels: a Proof of Concept Study

McGaughran, L.; Voss, Logan J.; Oliver, R. G.; Petcu, Miruna; Schaare, Peter; Barnard, John P.; Sleigh, James W.

doi:10.1007/s10877-006-9038-8

Cited by 10 publications

(11 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is capable of making a propofol concentration measurement in heparinised whole blood or plasma using automated solid phase extraction and detection. A minimum sample size of 0.5 ml heparinised whole blood is lysed with de‐ionised water in the ratio of 1:2 (blood:water) and filtered through a 1‐μm filter, followed by automated solid phase drug extraction [8]. Briefly, a sorbent cartridge (Phenomenex, Macclesfield, UK) is conditioned with methanol and equilibrated with water.…”

Section: Methodsmentioning

confidence: 99%

“…We assessed a device designed to measure the concentration of propofol in a blood sample in 4 min by extracting propofol from 0.5 ml of whole blood using solid phase extraction and quantifying the amount of propofol in the extract using colorimetric detection techniques. This technique for propofol extraction was first described in 2006 in a proof of concept study [8]. We conducted both laboratory and clinical evaluations of the device, the latter using samples from patients undergoing propofol‐based general anaesthesia for cardiac surgery.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of a new analyser for rapid measurement of blood propofol concentration during cardiac surgery

Cowley¹,

Laitenberger²,

Liu³

et al. 2012

Anaesthesia

View full text Add to dashboard Cite

SummaryWe report laboratory and clinical evaluations of a blood propofol concentration analyser. Laboratory experiments used volunteer blood spiked with known propofol concentrations over the clinically relevant concentrations from 0.5 to 16 lg.ml )1 to assess linearity and the influence of haematocrit and concurrent drug administration. Analyser concentrations demonstrated excellent linearity (R 2 = 0.999). Blood spiked with commonly used drugs showed no significant variation compared to unspiked controls. Propofol measurements were largely independent of haemoglobin concentration. A 6% decay in propofol concentration was observed at the highest prepared concentration. Clinical performance of the analyser was assessed using 80 arterial blood samples from 72 patients receiving propofol infusions during cardiac surgery. Samples were processed using the propofol analyser, and high performance liquid chromatography (HPLC) used as a gold-standard comparator. These data demonstrated excellent agreement between the propofol analyser and HPLC with a bias of 0.13 lg.ml )1 and precision of )0.16 to 0.42 lg.ml Target-controlled infusion (TCI) is a common method of propofol administration. The ability of TCI algorithms to predict blood propofol concentrations accurately is poor [1, 2], with measures of precision demonstrating errors of up to 60% [3]. Significant bias and large errors in precision are also identified in groups falling outside of limits used in the development of these algorithms [4][5][6]. Conventional methods of measuring propofol concentrations in blood include high performance liquid chromatography (HPLC), gas chromatography and liquid chromatography mass spectroscopy. These are labour intensive and time consuming methods and cannot be used to deliver clinically useful information. Estimation of blood propofol from expired gases has yet to demonstrate consistent and reliable results [7].We assessed a device designed to measure the concentration of propofol in a blood sample in 4 min by extracting propofol from 0.5 ml of whole blood using solid phase extraction and quantifying the amount of propofol in the extract using colorimetric detection techniques. This technique for propofol extraction was first described in 2006 in a proof of concept study [8]. We conducted both laboratory and clinical evaluations of the device, the latter using samples from patients undergoing propofol-based general anaesthesia for cardiac surgery.

show abstract

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

Evaluation of a new analyser for rapid measurement of blood propofol concentration during cardiac surgery

Cowley¹,

Laitenberger²,

Liu³

et al. 2012

Anaesthesia

View full text Add to dashboard Cite

show abstract

“…Previous reports [7,8] have identified several metabolites in human: 4-(2,6-diisopropyl-1,4-quinol) (quinol), 4-(2,6-diisopropyl-1,4-quinol) sulfate (4-QS), 1-(2,6-diisopropyl-1,4-quinol) glucuronide (1-QG), 4-(2,6-diisopropyl-1,4-quinol) glucuronide (4-QG) and propofol-glucuronide (PG) [8][9][10]. Occasionally, propofol was metabolized into minor compounds as x-((2-(-propanol)-6-isoproyl-phenol) glucuronide) (x-2--PG) and y-((2-(-propanol)-6-isopropyl-phenol) glucuronide) (y-2--PG).…”

Section: Introductionmentioning

confidence: 99%

“…Various analytical methods have been reported for the determination of propofol and its metabolites in biological matrices such as blood [9][10][11], urine [8,[12][13][14] and hair [15] including the use of high performance liquid chromatography (HPLC) with UV detection [4,7], and gas chromatography coupled mass spectrometry (GC/MS) [14,15]. In general, GC/MS methods have been used for the determination of propofol and its metabolites on account of the high separation capacity and detection sensitivity of the volatile compounds, even though intensive sample workup such as extraction and derivatization is required [14,16].…”

Section: Introductionmentioning

confidence: 99%

Comparison of GC/MS and LC/MS methods for the analysis of propofol and its metabolites in urine

Lee

Park

Jeong

et al. 2012

Journal of Chromatography B

View full text Add to dashboard Cite

“…T hree ‐ way stopcocks provide flexibility during anaesthesia to add an infusion of antibiotics, red blood cells, fresh‐frozen plasma and different drugs, especially total intravenous anaesthetics (TIVA) with propofol and remifentanil. Measurement of blood propofol levels is currently being investigated, 1 but currently there exists no validated non‐invasive, simple technique for monitoring anaesthesia drug blood concentrations. Although use of the bispectral‐index (BIS) 2 and entropy 3 may be an alternative to measure anaesthesia levels, it is controversially discussed, 4 and still not routine practice in the operating room.…”

mentioning

confidence: 99%