Rapid measurement of waterborne bacterial viability based on difunctional gold nanoprobe

“…Wen et al [4] developed a novel detection nanoprobe for the viability of bacteria in water (Figure1d). This nanoprobe is a difunctional gold nanoprobe, which through the color change, namely the colorimetric method, to show the detection effect.…”

Section: Bacteria Detectionmentioning

confidence: 99%

Biosensors Based on Gold Nano-Clusters

Chen

2023

TNS

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The world is entering an era of strong infectious diseases, and it is urgent to develop a new type of rapid, efficient detection system. This graph specifically illustrates the detection function of the gold nanoparticles, and the gold nanoparticles-based sensor. Gold-based nanoclusters (NCs) which has a small particle size, has a high degree of stability and no impact on the biological activity. Gold-based NCs has a surface plasma resonance effect and produces visible color, which can be detected by observing the color change using the agglomeration reaction of the detected substance with Gold-based NCs. In order to specifically detect certain biomolecules, some functional groups or small DNA molecular chains of nanolodin are usually modified on it to have a selective detection capability. In the review, we introduce a total of four nano-gold applications, for bacteria, viruses, DNA and cancer, also, respectively elaborating some recent Gold-based NCs detection applications.

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“…Up to date, gold nanoparticles (Au NPs) have been in use for a wide range of biomedical applications like drug delivery, disease treatment, optical imaging and catalysis owing to their physical and chemical properties. [1][2][3][4][5][6][7][8][9][10][11][12] In addition to that, Au NPs have been heavily incorporated to various sensing platform to colorimetrically detect a wide variety of targets including metal/nonmetals ions, [13][14][15][16] organic or biomolecules [17][18][19] and even virus, bacteria and also cancer cells [20][21][22][23][24][25][26] due its unique surface plasmon resonance (SPR) property. For instance, while the dispersed spherical Au NPs in solution gives wine-red color, when they are in close proximity each other, they are in tendency to aggregation and induce blue-purple color.…”

Section: Introductionmentioning

confidence: 99%

Development of a Colorimetric Urease Test Based on Au NPS Capped with Anthocyanin for the Rapid Detection of Helicobacter pylori through Multiple Readouts

Sezgin,

Tekin,

Calim

et al. 2023

ChemistrySelect

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In this work, we have reported use of anthocyanin capped gold nanoparticles (Ant@Au NPs)‐incorporated colorimetric urease test for rapid, sensitive and economic detection of Helicobacter pylori (H. pylori). This test containing Ant@Au NPs, urea, sodium phosphate buffer (PBS) and sodium azide (NaN3) was prepared at pH 5 with red solution owing to dispersity of Ant@Au NPs, urease enzyme secreted from H. pylori cause production of ammonia (NH3) via hydrolysis of urea, then which makes reaction environment alkaline (pH 8.2). The limit of detection for this Ant@Au NPs based urease test was determined to be to 102 CFU/mL with proportional to increasing incubation time. However, distinct color change was observed with 104 CFU/mL H. pylori suspensions in 15 min. The anthocyanin molecules existing on surface of Ant@Au NPs were easily deprotonated from hydroxyl groups in the alkaline condition, which caused various changes on Ant@Au NPs including quite much negative charges on the surface of Ant@Au NPs analyzed by Zeta potential, aggregation of Ant@Au NPs demonstrated by STEM, DLS and spectrophotometer, and turning the color of test solution to purple evaluated by a naked eye and digital process imaging system.

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Rapid measurement of waterborne bacterial viability based on difunctional gold nanoprobe

Abstract: A colorimetric method is proposed to measure waterborne bacterial viability by using a difunctional gold nanoprobe that can generate color signals while recognizing bacterial suspensions of different viabilities.

Cited by 6 publications

References 52 publications

Smartphone-based surface plasmon resonance sensing platform for rapid detection of bacteria

Smartphone-based surface plasmon resonance sensing platform for rapid detection of bacteria

Biosensors Based on Gold Nano-Clusters

Development of a Colorimetric Urease Test Based on Au NPS Capped with Anthocyanin for the Rapid Detection of Helicobacter pylori through Multiple Readouts

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