Rapid method for the detection and determination of artemisinin by gas chromatography

Sipahimalani, A. T.; Fulzele, Devanand P.; Heble, M. R.

doi:10.1016/s0021-9673(01)88868-3

Cited by 51 publications

(19 citation statements)

References 5 publications

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“…Sipahimalani et al presented a method for the detection and determination of artemisinin in plants and plant tissue cultures, in which a linear relationship was obtained between the concentration of artemisinin and the respective peak areas for either of its two thermally degraded products. In that particular study, no complete separation of arteannuin B from one of the decomposition products of artemisinin was achieved [48].…”

Section: Analytical Proceduresmentioning

confidence: 85%

Progress in the research of artemisinin-related antimalarials: An update

et al. 1994

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Artemisinin, a sesquiterpene lactone endoperoxide isolated from Artemisia annua L., and a number of its semisynthetic derivatives have shown to possess antimalarial properties. They are all effective against Plasmodium parasites that are resistant to the newest and commonly used antimalarial drugs. This article gives a survey of the literature dealing with artemisinin-related antimalarial issues that have appeared from the end of 1989 up to the beginning of 1994. A broad range of medical and pharmaceutical disciplines is covered, including phytochemical aspects like the selection of high-producing plants, analytical procedures, and plant biotechnology. Furthermore, the organic synthesis of artemisinin derivatives is discussed, as well as their mechanism of action and antimalarial activity, metabolism and pharmacokinetics, clinical studies, side-effects and toxicology, and biological activities other than antimalarial activity.

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Section: Analytical Proceduresmentioning

confidence: 85%

Progress in the research of artemisinin-related antimalarials: An update

et al. 1994

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“…Recently, Sipahimalani et al (1991) described a GC method for the analysis of 3, using a packed OV 17 column. Under the conditions applied only decomposition products of 3 were measured.…”

Section: Resultsmentioning

confidence: 99%

Analysis of artemisinin and related sesquiterpenoids from artemisia annua L. by combined gas chromatography/mass spectrometry

Woerdenbag

Pras

Bos

et al. 1991

Phytochemical Analysis

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The sesquiterpenoid artemisinin (3) and its biosynthetic precursors arteannuic acid (1), arteannuin B (2) and artemisitene (4) can be separated and identified by combined gas chromatography/mass spectrometry both as a mixture of reference standards as well as in extracts of Artemisia annua L. From this study a generally applicable gas chromatographic method has been developed for the analysis of these sesquiterpenoids in A. annua. Analysis of plant parts revealed that the highest sesquiterpenoid concentrations were present in the leaves, buds and small green stems, while the main stem contained only small amounts. Low levels of arteannuic acid and arteannuin B were present in the side‐roots. No sesquiterpenoids could be detected in the main root and none of the plant parts accumulated detectable quantities of artemisitene.

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“…The techniques include HPLC [4,5] with UV, ELSD, MS, [6] chemiluminescence, [7] and several GC methods with MS detection or other detection modes. [8,9] In this study, we developed a TLC=UV method for the simultaneous determination of artemisinin. Artemisia annua L. sample were supplied by Iranian Biological Resource Center (IBRC).…”

Section: Introductionmentioning

confidence: 99%

ANALYSIS OF ARTEMISININ ISOLATED FROM Artemisia Annua L. BY TLC AND HPLC

Ghafoori

Sariri

Naghavi

et al. 2013

Journal of Liquid Chromatography & Related Technologies

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& Artemisia annua L. (Quinghao) is a promising and potent source of antimalarial herbal drug. Its activity has been ascribed to the content of artemisinin; it has been analyzed by different chromatographic techniques. In this research, we have developed a TLC=UV, a simply available, rapid, and cost-efficient method, for the simultaneous determination of artemisinin. The crude extracts from plant samples were used together with a standard in our HPLC=UV and TLC= UV techniques. Artemisinin (standard and in the extract) was converted into a UV-absorbing compound, Q258 by being treated with 0.25% (by weight) NaOH solution and then 0.2 M acetic acid. The resulting TLC method utilizes separation on silica gel RP-18 with methanol, acetonitrile, ethyl acetate, acetic acid (30:20:2:1) as mobile phase, highly specific densitometric evaluation at 254 nm. This precise and accurate assay could be performed in the linear working range of 10-100 lg=mL and 1-40 lg=mL for TLC and HPLC methods, respectively. The new technique has enabled us to screen high artemisinin producing plants from a large number of samples.

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Rapid method for the detection and determination of artemisinin by gas chromatography

Cited by 51 publications

References 5 publications

Progress in the research of artemisinin-related antimalarials: An update

Progress in the research of artemisinin-related antimalarials: An update

Analysis of artemisinin and related sesquiterpenoids from artemisia annua L. by combined gas chromatography/mass spectrometry

ANALYSIS OF ARTEMISININ ISOLATED FROM Artemisia Annua L. BY TLC AND HPLC

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