2010
DOI: 10.1128/aem.00743-10
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Rapid Microarray-Based Genotyping of Enterohemorrhagic Escherichia coli Serotype O156:H25/H−/Hnt Isolates from Cattle and Clonal Relationship Analysis

Abstract: Since enterohemorrhagic Escherichia coli (EHEC) isolates of serogroup O156 have been obtained from human diarrhea patients and asymptomatic carriers, we studied cattle as a potential reservoir for these bacteria. E. coli isolates serotyped by agglutination as O156:H25/H؊/Hnt strains (n ‫؍‬ 32) were isolated from three cattle farms during a period of 21 months and characterized by rapid microarray-based genotyping. The serotyping by agglutination of the O156 isolates was not confirmed in some cases by the resul… Show more

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Cited by 45 publications
(40 citation statements)
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“…We found S. aureus at lower prevalences of 29.4% in sheep and 11.5% in goats. These strains all belonged to the same clonal complex (CC133), which has been predominant in isolates from small ruminants in other studies (17,20). They carry the leukocidin lukM-lukF P83, a toxin host specific for ruminants (35).…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…We found S. aureus at lower prevalences of 29.4% in sheep and 11.5% in goats. These strains all belonged to the same clonal complex (CC133), which has been predominant in isolates from small ruminants in other studies (17,20). They carry the leukocidin lukM-lukF P83, a toxin host specific for ruminants (35).…”
Section: Discussionmentioning
confidence: 99%
“…The primer elongation and the hybridization, washing, and straining of ArrayStrips were described previously (17). The ArrayStrips were photographed using an ArrayMate instrument (Alere Technologies, Jena, Germany) and automatically analyzed.…”
Section: Methodsmentioning
confidence: 99%
“…In contrast, a standard commercial kit for DNA isolation takes Ͼ1 h. We observed no significant differences in the signal and background quality of the microarray between the methods, which allows the conclusion that the DNA isolated by heat lysis is sufficiently pure and highly concentrated (0.5 to 1.5 g) in order to be used in the standard protocol for labeling and hybridization. A further advantage of the microarray is that the number of spotted probes on the array can be extended by additional probes, e.g., for resistance genes or markers for further characterization of E. coli or the identification of other Gramnegative bacteria that we have described before (12,(25)(26)(27).…”
Section: Discussionmentioning
confidence: 99%
“…The two French isolates carried none of the resistance genes covered by the array (9). The 2004 isolate was completely susceptible to nalidixic acid, whereas the 2009 isolate was resistant.…”
Section: Two Isolates Of Enterohemorrhagic Escherichia Coli (Ehec)mentioning
confidence: 99%