Rapid monitoring of benzylpenicillin sodium using Raman and surface enhanced Raman spectroscopy

Jiang, Xin; Qin, Xiaoyu; Yin, Di; Gong, Mengdi; Yang, Libin; Zhao, Bing; Ruan, Weidong

doi:10.1016/j.saa.2015.01.016

Cited by 35 publications

(17 citation statements)

References 31 publications

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“…The stronger band in this spectrum is observed at 1000 cm −1 , which is a Raman signature of PG, though its assignment has not been consensual in the literature. Hence, some authors have assigned this Raman band to the phenyl ring vibrational modes [8,9,12,39,40,42], while others assigned it to the presence of the beta lactam ring [11,38,41,44]. Nevertheless, for the magnetic-plasmonic substrates employed here, which differ in the average size of the Au NPs adsorbed at the surface, the SERS spectra always showed the band at about 1000 cm −1 .…”

Section: Resultsmentioning

confidence: 82%

“…Penicillin G (PG) belongs to the β-lactam antibiotics family whose structure is composed of a five-membered sulfur-containing thiazolidine ring fused to a beta-lactam ring and a phenyl ring attached to the side chain ( Figure S1, Supporting Information). The intensive use of PG as a pharmaceutical associated with the potential contamination of waters has raised interest in the SERS analysis of this antibiotic [8,9,11,12,[38][39][40][41][42][43][44]. To the best of our knowledge, there are no SERS studies on this analyte using magneto-plasmonic substrates.…”

Section: Introductionmentioning

confidence: 99%

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SERS Detection of Penicillin G Using Magnetite Decorated with Gold Nanoparticles

2017

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Sensitive and reliable procedures for detecting vestigial antibiotics are of great relevance for water quality monitoring due to the occurrence of such emergent pollutants in the aquatic environment. As such, we describe here research concerning the use of multifunctional nanomaterials combining magnetic and plasmonic components. These nanomaterials have been prepared by decorating magnetite nanoparticles (MNP) with colloidal gold nanoparticles (Au NPs) of distinct particle size distributions. Several analytical conditions were investigated in order to optimize the surface enhanced Raman scattering (SERS) detection of penicillin G (PG) dissolved in water. In particular, the dependence of the SERS signal by using distinct sized Au NPs adsorbed at the MNP was investigated. Additionally, microscopic methods, including Raman confocal microscopy, were employed to characterize the SERS substrates and then to qualitatively detect penicillin G using such substrates. For example, magnetic-plasmonic nanocomposites can be employed for magnetically concentrate analyte molecules and their removal from solution. As a proof of concept, we applied magneto-plasmonic nanosorbents in the removal of aqueous penicillin G and demonstrate the possibility of SERS sensing this antibiotic.

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Section: Resultsmentioning

confidence: 82%

Section: Introductionmentioning

confidence: 99%

SERS Detection of Penicillin G Using Magnetite Decorated with Gold Nanoparticles

2017

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“…SERS studies were applied to penicillin derivatives, trihydrate amoxicillin, levofloxacin, nitroimidazole derivatives, ciprofloxacin, sulfamethoxazole, chloramphenicol and tetracycline , . Zhao and co‐workers have fabricated sulfonated poly(styrene) microspheres decorated with Ag NPs, that show potential for the trace detection of penicillin G and chloramphenicol .…”

Section: Sers and Water Quality Analysismentioning

confidence: 99%

Functionalized Inorganic Nanoparticles for Magnetic Separation and SERS Detection of Water Pollutants

et al. 2018

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Inorganic nanoparticles (NPs) have emerged in the last decades as key materials for a number of technologies. In particular, noble metal (Ag, Au) and iron oxide NPs have revealed important applications due to their plasmonic and magnetic properties, respectively. Among these applications, this review provides, on one hand, an overview on the use of colloidal metal NPs in surface enhanced Raman scattering (SERS) chemical analysis and, on the other hand, the application of iron oxides as a new class of nanosorbents for water pollutants. These distinct types of NPs are the main building blocks to produce [a] Raman spectroscopy is a powerful analytical tool for chemical characterization, providing information about molecular structure, surface processes, and interface reactions. [31,32] Raman spectroscopy is based on the inelastic scattering of photons by chemical species and materials (Raman scattering). Among the incident photons that hit the sample only a small portion is inelastically scattered, i.e. comes out at a different frequency from the incident light. The spectral information is based on the detection of these scattered photons that are recorded as energy shifts (Raman shifts) from the wavenumber of the elastic scattered photons (Rayleigh scattering). Each band in the Raman spectrum can be assigned to a vibrational mode (or Paula

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“…Using peak 329 height relative to the background signal (10-20 wavennumbers away from the peak), 330 peaks were identified which showed clear decay over time. These peaks were assigned 331 using Biorad's KnowItAll software, combined with a literature survey [47][48][49][50][51][52][53][54][55]. One peak 332 of interest was determined (see Fig 4C): 1278 cm −1 (thought to be due to twisting of 333 C-N-C [47], or twisting of the CH 2 group [55])).…”

mentioning

confidence: 99%

“…These peaks were assigned 331 using Biorad's KnowItAll software, combined with a literature survey [47][48][49][50][51][52][53][54][55]. One peak 332 of interest was determined (see Fig 4C): 1278 cm −1 (thought to be due to twisting of 333 C-N-C [47], or twisting of the CH 2 group [55])). The peak at 1278 cm −1 was identified 334 as important as a Biorad's KnowItAll database search of possible mecillinam 335 degradation products (e.g.…”

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confidence: 99%

Stability ofβ-lactam antibiotics in bacterial growth media

Brouwers

Vass

Dawson

et al. 2020

Preprint

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Laboratory assays such as MIC tests assume that antibiotic molecules are stable in the chosen growth medium -but rapid degradation has been observed for antibiotics including β-lactams under some conditions in aqueous solution. Degradation rates in bacterial growth medium are less well known. Here, we develop a 'delay time bioassay' that provides a simple way to estimate antibiotic stability in bacterial growth media. We use the bioassay to measure degradation half-lives of the β-lactam antibiotics mecillinam, aztreonam and cefotaxime in widely-used bacterial growth media based on MOPS and Luria-Bertani (LB) broth. We find that mecillinam degradation can occur rapidly, with a half-life as short as 2 hours in MOPS medium at 37 • C and pH 7.4, and 4-5 hours in LB, but that adjusting the pH and temperature can increase its stability to a half-life around 6 hours without excessively perturbing growth. Aztreonam and cefotaxime were found to have half-lives longer than 6 hours in MOPS medium at 37 • C and pH 7.4, but still shorter than the timescale of a typical minimum inhibitory concentration (MIC) assay. Taken together, our results suggest that care is needed in interpreting MIC tests and other laboratory growth assays for β-lactam antibiotics, since there may be significant degradation of the antibiotic during the assay.Antibiotic efficacy is usually quantified by the minimal inhibitory concentration (MIC), 2 or the concentration of antibiotic needed to prevent bacterial growth over 24 hours in a 3 standard laboratory growth medium [1]; the MIC value plays a central role in 4 diagnostic and therapeutic decision-making. When performing an MIC assay, one 5 assumes that the antibiotic does not degrade over the timescale of the assay. Antibiotic 6 stability is also assumed in a host of other bacterial growth assays that are used to 7 determine antibiotic mechanism of action [2][3][4][5], interactions between antibiotics [6-9], 8 and evolution of antibiotic resistance [10][11][12][13][14]. 9Antibiotic degradation in aqueous solution has been extensively studied in the 10 chemical literature [15][16][17][18][19], and it is well-known that, under some conditions, 11 antibiotics can degrade on timescales much shorter than a typical bacterial growth assay. 12 There has been little work, however, on how quickly antibiotics degrade in standard 13 laboratory growth media, such as those used for MIC assays. Here, we develop a 14 'delay-time bioassay', based on growth measurements in a plate reader, that allows 15 simple estimation of the rate of antibiotic degradation in bacterial growth media. 16Bacterial growth media are chemically complex, since bacterial proliferation requires 17 carbon, nitrogen, phosphorous, iron and a diverse array of micronutrients [20,21]. 18April 14, 2020 1/18 "Undefined" growth media contain ingredients such as yeast extract, blood or beef 19 extract, whose detailed chemical composition is not known. Luria-Bertani (LB) medium, 20 which consists of water, tryptone, sodium chloride and yeast extract, i...

show abstract

Rapid monitoring of benzylpenicillin sodium using Raman and surface enhanced Raman spectroscopy

Cited by 35 publications

References 31 publications

SERS Detection of Penicillin G Using Magnetite Decorated with Gold Nanoparticles

SERS Detection of Penicillin G Using Magnetite Decorated with Gold Nanoparticles

Functionalized Inorganic Nanoparticles for Magnetic Separation and SERS Detection of Water Pollutants

Stability ofβ-lactam antibiotics in bacterial growth media

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