Rapid multiplication of Polyscias filicifolia by secondary somatic embryogenesis

Abstract

Efficient plant regeneration through somatic embryogenesis was achieved in Polyscias filicifolia. Embryogenic calluses were induced on Murashige and Skoog (MS) basal medium supplemented with 0.5 mg l −1 2,4-dichlorophenoxyacetic acid (2,4-D) and 1.0 mg l −1 benzylaminopurine (BAP; type I callus) and on MS medium with 2.0 mg l −1 2,4-D and 0.01 mg l −1 kinetin (type II callus) from leaf explants of a 2-yr-old plant. Primary somatic embryos (PSEs) developed after four passages of suspension culture established from embryogenic callus when cultured in liquid half-strength MS medium (1/2 MS) without growth regulators. PSEs in the cotyledonary stage were multiplied by adventitious embryogenesis. Single secondary somatic embryos (SSEs) or their clusters developed at the base of PSE hypocotyls and regenerated into plantlets in a one-step process on plant growth regulator-free 1/2 MS medium. Low sucrose concentration of 15 g l −1 promoted development of normal SSEs. All SSEs regenerated into single, well-rooted plantlets on a Nitsch and Nitsch medium supplemented with 0.5 mg l −1 kinetin, 0.1 mg l −1 indole-3-butyric acid, and 10 mg l −1 adenine sulfate. Subsequent two subculture cycles on the same medium were necessary to obtain plantlets sufficiency developed to allow successful transfer to the soil. Rooted plantlets were established in a peat mixture with 90% survival, with the plants showing normal morphological characteristics.

Stimulation of phenolic compounds production in the in vitro cultivated Polyscias filicifolia Bailey shoots and evaluation of the antioxidant and cytotoxic potential of plant extracts

“…Polyscias filicifolia shoots were obtained by micropropagation of plants regenerated from somatic embryos, as described by Śliwińska et al [24]. These shoots were cultivated on Linsmaier and Skoog (LS) medium [25] supplemented with 2.0 mg/L 6-benzylaminopurine (BAP) and 0.5 mg/L kinetin (KIN), 30 g/L sucrose, and 8.0 g/L Plant Propagation Agar (Biocorp, Poland) and passaged every 6 weeks onto fresh media, and then used as a plant material [24]. The shoots of P. filicifolia cultured on LS medium for 5 weeks were transferred onto LS medium supplemented with elicitors: JM and SA at concentrations of 50, 100, or 200 µM.…”

In Vitro Response of Polyscias filicifolia (Araliaceae) Shoots to Elicitation with Alarmone–Diadenosine Triphosphate, Methyl Jasmonate, and Salicylic Acid

“…The studied explants were P. filicifolia plantlets developed as described by Śliwińska et al [ 28 ]. The plantlets were cultured on 1/3 strength MS [ 29 ] solid medium without growth regulators for three months.…”

“…The plantlets were cultured on 1/3 strength MS [ 29 ] solid medium without growth regulators for three months. Following this, their apical parts were isolated (3.5–4.5 cm length) and multiplied in the first stage on LS [ 30 ] solid medium supplemented with 8.88 µM (2 mg/L) 6-benzylaminopurine (BAP) and 2.32 µM (0.5 mg/L) kinetin (KIN), 30 g/L sucrose and cultivated for five weeks at 16 h/8 h (light/dark) photoperiod with light provided by cool-white fluorescent lamps (40 µM/m 2 /s 1 ) at 25 ± 1 °C [ 28 ]. BAP and KIN were added to the medium prior to autoclaving.…”

Comparative Study of the Genetic and Biochemical Variability of Polyscias filicifolia (Araliaceae) Regenerants Obtained by Indirect and Direct Somatic Embryogenesis as a Source of Triterpenes

“…P. filicifolia is a species that shares the genus Polyscias with between 116 [ 1 ] and 150 others [ 2 ]. It is an evergreen, six-meter-tall shrub that naturally occurs in Southeast Asia, but is also available as a decorative potted plant [ 3 ]. Polyscias spp.…”