Rapid naked-eye detection of Gram-positive bacteria by vancomycin-based nano-aggregation

Shin, Cheong; Lee, Ha Neul; Ryu, Jea Sung; Chung, Hyun Jung

doi:10.1039/c8ra03540g

Cited by 8 publications

(3 citation statements)

References 48 publications

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“…Also, the opposite effect of dendrons (i.e., bacterial aggregation support when bacteria were first treated with dendron 4 and subsequently with AMP3) that was also observed in this study could be interesting because bacterial clustering can in some cases complicate attack on target cells by these bacteria, and may eventually somehow reduce the spread of bacteria in a given organism. , In addition, this phenomenon can also be used for the bacterial detection and diagnostic purposes …”

Section: Resultsmentioning

confidence: 56%

“…Finally, let us note that the ability to aggregate bacteria per se can also be in some cases understood as certain antibacterial activity prevents or complicates host cell attack 34 or it could be useful for bacterial detection and diagnostic purpose. 38 From this point of view, synergic effect found after sequential application of first dendrons and second AMP3 might be of particular interest due to the increase of AMP3 aggregation ability achieved.…”

Section: Confocal Microscopymentioning

confidence: 99%

“…34,35 In addition, this phenomenon can also be used for the bacterial detection and diagnostic purposes. 38…”

Section: Molecular Pharmaceuticsmentioning

confidence: 99%

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Carbosilane Dendron–Peptide Nanoconjugates as Antimicrobial Agents

et al. 2019

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Over the last decades, multidrug-resistant bacteria have emerged and spread, increasing the number of bacteria, against which commonly used antibiotics are no longer effective. It has become a serious public health problem whose solution requires medical research in order to explore novel effective antimicrobial molecules. On the one hand, antimicrobial peptides (AMPs) are regarded as good alternatives because of their generally broad-spectrum activities, but sometimes they can be easily degraded by the organism or be toxic to animal cells. On the other hand, cationic carbosilane dendrons, whose focal point can be functionalized in many different ways, have also shown good antimicrobial activity. In this work, we synthetized first- and second-generation cationic carbosilane dendrons with a maleimide molecule on their focal point, enabling their functionalization with three different AMPs. After different microbiology studies, we found an additive effect between first-generation dendron and AMP3 whose study reveals three interesting effects: (i) bacteria aggregation due to AMP3, which could facilitate bacteria detection or even contribute to antibacterial activity by preventing host cell attack, (ii) bacteria disaggregation capability of second-generation cationic dendrons, and (iii) a higher AMP3 aggregation ability when dendrons were added previously to peptide treatment. These compounds and their different effects observed over bacteria constitute an interesting system for further mechanism studies.

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Section: Resultsmentioning

confidence: 56%

Section: Confocal Microscopymentioning

confidence: 99%

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Carbosilane Dendron–Peptide Nanoconjugates as Antimicrobial Agents

et al. 2019

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Polydopamine Sensors of Bacterial Hypoxia via Fluorescence Coupling

Lee

Ryu

Kang

et al. 2020

Adv Funct Materials

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Biological catecholamines play critical physiological roles in various parts of the human body, namely, the skin and brain. In the skin, an oxygen‐contacting and oxygen‐abundant body part, catecholamine molecules are oxidatively polymerized, becoming melanin. In contrast, the brain is an oxygen‐demanding organ that suppresses catecholamine oxidation. Catecholamine oxidative polymerization, also known as polydopamine (or dopamine–melanin) formation, can be finely controlled by bacterial growth. Under exponential growth of Escherichia coli, a process that requires large amounts of oxygen, dopamine polymerization is significantly inhibited. In contrast, under steady‐state growth, polydopamine is formed due to the abundance of oxygen which is not actively consumed by E. coli. This polydopamine‐oxygen relationship is further demonstrated by using fluorescent dextran nanoparticles (FDNPs) as sensors, whose fluorescence is quenched by polydopamine formation. Thus, FDNP fluorescence can be precisely controlled by dopamine concentration, incubation time, and bacterial number. The cascade coupling of E. coli growth—oxygen level—polydopamine—fluorescence can also be used to detect the antibiotic‐resistant bacteria, New Delhi metallo‐beta‐lactamase 1‐positive (NDM1+) E. coli. This method not only uncovers the unique role played by biological catecholamine in a living system, but also presents a diagnostic assay for detecting bacterial growth and antibiotic susceptibility.

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