Rapid, Noninvasive Quantitative Determination of Acyclovir in Pharmaceutical Solid Dosage Forms through Their Poly(Vinyl Chloride) Blister Package by Solid-State Fourier Transform Raman Spectroscopy

Abstract

Fourier transform (FT) Raman spectroscopy based on band intensity or band area measurements was used for the quantitative determination of acyclovir in pharmaceutical solid dosage forms through their poly(vinyl chloride) blister package. Univariate calibration using the bands observed at 1690, 1630, 1574, 1482, 1181, 578, and 508 cm(-1) was found to be sufficient for the analysis. Calibration curves were linear, the correlation coefficients being 0.997-0.9993 and 0.996-0.9991 for band intensity and band area measurements, respectively. Results obtained compare well, as indicated by the t-test, with those obtained by the current United States Pharmacopoeia (USP 24) and National Formulary (NF 19) method. Precision ranged from 0.7-4.5 and 0.4-4.0% RSD (n = 3) for band intensity and band area measurements, respectively. The developed nondestructive FT-Raman method is rapid, simple, and can be used for the on-line, real-time monitoring of acyclovir formulation production lines.

Reference database of Raman spectra of pharmaceutical excipients

“…[1] While using Raman spectroscopy, no sample preparation is necessary, a Raman microscope allows to study very small particles (down to 1 µm) and it is even possible to measure through blister packages. [2] Raman spectroscopy has been used as an important research tool for different domains in the pharmaceutical world, for instance in quantitative as well as qualitative analysis. [3] In the past, this technique has been used for the identification of active ingredients and their different polymorphic crystalline forms.…”

High-throughput HPLC assay of acyclovir and its major impurity guanine using a monolithic column and a flow gradient approach

“…Flow injection analysis with either chemiluminescence [4] or electro-chemiluminescence [5] detection schemes, a non-destructive method based on FT-Raman spectroscopy [6], and a derivative spectrophotometric assay developed by Daabees [7]. Separation techniques are usually the techniques of choice in pharmaceutical analysis and quality control mainly due to the possibility of multi-analytes deter- * Corresponding author.…”

Quantitative determination of captopril and prednisolone in tablets by FT-Raman spectroscopy

“…Unlike many other common analytical methods, this technique does not require any special sample preparation, so analysed drugs are usually in their unmodified forms. Often it is possible to determine active pharmaceutical ingredients (API) quantitatively in polymer blisters or in ampoules, what significantly simplifies the analysis [21,22].…”