2021
DOI: 10.1021/acscentsci.1c01205
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Rapid On-Cell Selection of High-Performance Human Antibodies

Abstract: Phage display is a critical tool for developing antibodies. However, existing approaches require many time-consuming rounds of biopanning and screening of potential candidates due to a high rate of failure during validation. Herein, we present a rapid on-cell phage display platform which recapitulates the complex in vivo binding environment to produce high-performance human antibodies in a short amount of time. Selection is performed in a highly stringent heterogeneous mixture of cells t… Show more

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Cited by 11 publications
(9 citation statements)
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“…C Schematic illustration of the μCellect methodology. Copyright 2022 American Chemical Society [54] the collision-based force effect with the two-dimensional separation-based concentration effect to increase the biopanning stringency, phages with high-affinity can be screened within only 6 h (Fig. 3B) [53].…”
Section: Phage Displaymentioning
confidence: 99%
See 1 more Smart Citation
“…C Schematic illustration of the μCellect methodology. Copyright 2022 American Chemical Society [54] the collision-based force effect with the two-dimensional separation-based concentration effect to increase the biopanning stringency, phages with high-affinity can be screened within only 6 h (Fig. 3B) [53].…”
Section: Phage Displaymentioning
confidence: 99%
“…The biopanning stringency can be tuned by changing the ratio of cell types. With the assistance of machine learning and next-generation sequencing (NGS), binders with picomolar affinity can be identified with only two rounds of biopanning [54].…”
Section: Phage Displaymentioning
confidence: 99%
“…Philpott et al presented a biopanning platform with a throughput of 6 × 10 7 cells per h (μCellect), and high-affinity antibodies against human Frizzled-7 were discovered in two rounds of selection. [81] Similarly, microfluidic panning has been optimized to identify high-affinity multiple-target phage display peptides in one round without requiring any bacterial culture. [82] The entire screening completed in only 4 h, resulting in less time and labor consumption.…”
Section: Phage Display Technologymentioning
confidence: 99%
“…Recent examples largely include genetically-encoded discovery methods, where round-to-round enrichment serves as the label in supervised learning analysis. [36][37][38] In contrast to genetically-encoded platforms, AS-MS has unparalleled use of abiotic chemical libraries, meaning its datasets could enable the development of an ML approach to broadly connect chemical space. AS-MS has historically been a screening tool; 39 but has been advanced with target-focused libraries, 16,40 and onward to de novo discovery with peptidomimetics against multiple biomolecular targets with large libraries (>10 8 members).…”
Section: Introductionmentioning
confidence: 99%