Rapid, on-site quantitative determination of higenamine in functional food using a time-resolved fluorescence microsphere test strip

Monoclonal antibody production and development of immunochromatographic strip assays for screening of the herbicide bispyribac-sodium in rice

“…25 The preparation of GNPs was the same as the previous study. 26,27 The GNP-mAb conjugate was prepared by a physical adsorption and condensation reaction according to previous references, 28 with some modifications. The process of labeling mAbs with GNP was as follows: 0.1 M K 2 CO 3 was added to 9 mL GNP solution to adjust the pH to 8.0 and then the anti-BIS mAb (50 μg) was added into the solution.…”

Development of a colloidal gold strip assay for the detection of total homocysteine in serum samples

“…The immunization process was performed as previously described. 23 Briefly, five 6–8-week-old female mice were immunized by multipoint subcutaneous injection with immunogen. The immunogen was prepared by emulsifying 100 μg of SAH-CDI-KLH with an equivalent volume of FCA for the first immunization.…”

Development and evaluation of time-resolved rapid immunofluorescence test for detection of TSOL18 specific antibody in porcine cysticercosis infections

“…Time-resolved fluorescence microsphere immunochromatography strip test (TRFM-ICST) is a relatively novel detection technique. The time-resolved lanthanide chelated microspheres have a longer fluorescence signal lifetime, with a long decay time of 10-2000 µs, about 10 3 times that of traditional fluorescent compared with traditional fluorescent microspheres [ 18 , 19 ]. The fluorescence signal of TRFM can still be measured after a certain excitation time interval, which avoids the influence of background fluorescence signal [ 18 , 19 ].…”

“…The time-resolved lanthanide chelated microspheres have a longer fluorescence signal lifetime, with a long decay time of 10-2000 µs, about 10 3 times that of traditional fluorescent compared with traditional fluorescent microspheres [ 18 , 19 ]. The fluorescence signal of TRFM can still be measured after a certain excitation time interval, which avoids the influence of background fluorescence signal [ 18 , 19 ]. However, TRFM-ICST can be read under a 365 nm wavelength UV light and analyzed through an immunoassay analyzer.…”

“…TRFM contains carboxyl groups with appropriate density, which can be used for covalently coupling of proteins or antibodies to improve the stability of the labeling [ 20 ]. Moreover, TRFM-ICST offers a series of advantages, including high specificity, sensitivity and stability, wide linear range, multi-marker detection, etc [ 18 – 20 ].…”