2017
DOI: 10.1186/s12915-017-0348-8
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Rapid, precise quantification of bacterial cellular dimensions across a genomic-scale knockout library

Abstract: BackgroundThe determination and regulation of cell morphology are critical components of cell-cycle control, fitness, and development in both single-cell and multicellular organisms. Understanding how environmental factors, chemical perturbations, and genetic differences affect cell morphology requires precise, unbiased, and validated measurements of cell-shape features.ResultsHere we introduce two software packages, Morphometrics and BlurLab, that together enable automated, computationally efficient, unbiased… Show more

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Cited by 136 publications
(151 citation statements)
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“…We grew the library in a 96-well plate and acquired phase images for exponentially growing cells using SLIP (Fig. 3b), then used Morphometrics 17 to extract quantitative morphological information from these images for direct comparison across strains. After correcting for the cell growth effect, mean cell length and width were highly correlated (Fig.…”
Section: Anticipated Resultsmentioning
confidence: 99%
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“…We grew the library in a 96-well plate and acquired phase images for exponentially growing cells using SLIP (Fig. 3b), then used Morphometrics 17 to extract quantitative morphological information from these images for direct comparison across strains. After correcting for the cell growth effect, mean cell length and width were highly correlated (Fig.…”
Section: Anticipated Resultsmentioning
confidence: 99%
“…Our laboratory has developed a custom MATLAB software, Morphometrics , for segmenting and analyzing phase images of bacteria cells 17 . General steps to use the software are included below; other software packages, e.g.…”
Section: Methodsmentioning
confidence: 99%
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