Rapid quantification of 14 saponins of Maesa lanceolata by UPLC-MS/MS

Foubert, Kenn; Cuyken, F; Theunis, Mart; Pollier, Jacob; González-Guzmán, Miguel; Lambert, Ellen; Geelen, Danny; Goossens, Annick; Vlietinck, A; Pieters, Luc; Apers, Sandra

doi:10.1055/s-0029-1234317

Cited by 4 publications

(5 citation statements)

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“…Relative standard deviation between results obtained for three independent extractions ranged from 3 to 16%. Considering difficulties in obtaining homogeneous plant material and that purification of samples included several steps of extraction/purification, the relative standard variation was satisfactory and was comparative to that obtained in our previous studies and to works performed in different laboratories. , …”

Section: Resultssupporting

confidence: 61%

Triterpene Saponin Content in the Roots of Red Beet (Beta vulgaris L.) Cultivars

Mroczek

Kapusta

Janda

et al. 2012

J. Agric. Food Chem.

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Triterpene saponins in the roots of Beta vulgaris cultivars Red Sphere, Rocket, and Wodan were profiled and quantitated using reverse-phase liquid chromatography coupled with electrospray ionization mass spectrometry (HPLC-ESI/MS/MS). Results obtained indicated that the roots of all three cultivars contained 11 saponins consisting of oleanolic acid or hederagenin aglycone and varying numbers of sugars, with the dominant triglycoside derivative of oleanolic acid. The relative proportions of derivatives of these two aglycones were similar in the three subspecies: cv. Red Sphere contained 99.1 and 0.9%; cv. Rocket, 98.2 and 1.8%; and Wodan 98.8 and 1.2% of oleanolic acid and hederagenin glycosides, respectively. To the best of our knowledge this is the first report on the occurrence, structure, and content of triterpenoid saponins in red beet.

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Section: Resultssupporting

confidence: 61%

Triterpene Saponin Content in the Roots of Red Beet (Beta vulgaris L.) Cultivars

Mroczek

Kapusta

Janda

et al. 2012

J. Agric. Food Chem.

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“…Results in rows not sharing a common letter are significantly different (p≤0.05); nd -not detected. The numbers (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16) correspond to the numbers of compounds named in Table 1.…”

Section: Orcid Idsmentioning

confidence: 99%

“…Consequently, appropriate methods needed to be established for the qualitative and quantitative analysis of saponins in complex plant matrices. Mass spectrometry (MS), as an extremely sensitive and specific analytical technique, providing qualitative and quantitative data, is an essential tool for saponin characterization [Foubert et al, 2010;Ge et al, 2017;Gómez--Caravaca et al, 2011;Kowalczyk et al, 2011;Mikołajczyk--Bator et al, 2016a, 2016b]. In the current study, we used ultra--performance liquid chromatography (UHPLC) coupled with quadrupole-time-of-flight mass spectrometry (QTOF-MS/MS) that has recently been widely applied to characterize natural compound structures, including saponins [Alara et al, 2018;Mikołajczyk-Bator et al, 2016a;Onlom et al, 2017].…”

Section: Introductionmentioning

confidence: 99%

Determination of Saponins in Leaves of Four Swiss Chard (<i>Beta vulgaris</i> L.) Cultivars by UHPLC-CAD/QTOF-MS/MS

Mroczek¹,

Klimczak²,

Kowalczyk³

2021

Pol. J. Food Nutr. Sci.

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Swiss chard is a vegetable valued not only for the taste of its leaves but also because of its health-promoting properties. To date, nothing is known regarding the occurrence of saponins in the Swiss chard plant, even though they could be at least partially responsible for the nutraceutical activities of this plant. This research aimed to describe saponins from the leaves of four Swiss chard (Beta vulgaris L.) cultivars. Saponin structures were analyzed by UHPLC-CAD/QTOF-MS/MS. Based on the fragmentation patterns, we tentatively identified 16 triterpene saponins in B. vulgaris, including two that had not been detected previously. The observed compounds were glycosides of five different, tentatively identified aglycones, i.e., oleanolic acid, hederagenin, gypsogenin, akebonoic acid, and serjanic acid. Moreover, the structure of four saponins detected in Swiss chard leaves included dioxolane-type and six acetal-type substituents. Eleven, eight, eleven, and eight saponins were observed in saponin fractions obtained from Rhubarb, Bulls Blood, Perpetual Spinach, and White Silver cultivars, respectively. Furthermore, the content of all identified triterpene derivatives in the investigated cultivars was estimated using a method based on the UHPLC coupled with QTOF-MS/MS and charged aerosol detector (CAD). The analyzed cultivars differed in the total and individual saponin content. The total saponin content ranged from 125.53 to 397.09 μg/g DW.

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“…It is also used in East African folk medicine to prevent cholera (Foubert et al, 2010). The antimicrobial activity observed in the extracts of Maesa lanceolata could be from the saponins, reportedly isolated from its leaves and have been shown to have virucidal, haemolytic and anthelmitic activities (Apers et al, 1998).…”

Section: Antibacterial Bioassaymentioning

confidence: 99%