Rapid Quantification of Escherichia coli in Potable Water by Fluorescence In Situ Hybridization Performed in Liquid (liq-FISH) and a Microfluidic System

Yamaguchi, Naohito; Goto, Satoko

doi:10.1007/s11270-019-4342-3

Cited by 3 publications

(1 citation statement)

References 17 publications

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“…Additionally, water samples were analyzed in regard to the occurrence of specific microorganisms. FISH was applied to tap water samples to detect E. coli [ 163 ], C. coli , and Mycobacterium avium [ 164 , 165 ]. However, most studies dealing with FISH for water samples have focused on seawater and lake water samples.…”

Section: Specific State-of-the-art Flow-fish Methods and Applications For Monitoring And Detectionmentioning

confidence: 99%

Potential of Flow Cytometric Approaches for Rapid Microbial Detection and Characterization in the Food Industry—A Review

Zand

Froehling

Schoenher

et al. 2021

Foods

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As microbial contamination is persistent within the food and bioindustries and foodborne infections are still a significant cause of death, the detection, monitoring, and characterization of pathogens and spoilage microorganisms are of great importance. However, the current methods do not meet all relevant criteria. They either show (i) inadequate sensitivity, rapidity, and effectiveness; (ii) a high workload and time requirement; or (iii) difficulties in differentiating between viable and non-viable cells. Flow cytometry (FCM) represents an approach to overcome such limitations. Thus, this comprehensive literature review focuses on the potential of FCM and fluorescence in situ hybridization (FISH) for food and bioindustry applications. First, the principles of FCM and FISH and basic staining methods are discussed, and critical areas for microbial contamination, including abiotic and biotic surfaces, water, and air, are characterized. State-of-the-art non-specific FCM and specific FISH approaches are described, and their limitations are highlighted. One such limitation is the use of toxic and mutagenic fluorochromes and probes. Alternative staining and hybridization approaches are presented, along with other strategies to overcome the current challenges. Further research needs are outlined in order to make FCM and FISH even more suitable monitoring and detection tools for food quality and safety and environmental and clinical approaches.

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Section: Specific State-of-the-art Flow-fish Methods and Applications For Monitoring And Detectionmentioning

confidence: 99%

Potential of Flow Cytometric Approaches for Rapid Microbial Detection and Characterization in the Food Industry—A Review

Zand

Froehling

Schoenher

et al. 2021

Foods

View full text Add to dashboard Cite

show abstract

Microfluidics in environmental analysis: advancements, challenges, and future prospects for rapid and efficient monitoring

Aryal,

Hefner,

Martinez

et al. 2024

Lab Chip

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Recent advances in microfluidic-based spectroscopic approaches for pathogen detection

Hussain,

He,

Wang

et al. 2024

Biomicrofluidics

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Rapid identification of pathogens with higher sensitivity and specificity plays a significant role in maintaining public health, environmental monitoring, controlling food quality, and clinical diagnostics. Different methods have been widely used in food testing laboratories, quality control departments in food companies, hospitals, and clinical settings to identify pathogens. Some limitations in current pathogens detection methods are time-consuming, expensive, and laborious sample preparation, making it unsuitable for rapid detection. Microfluidics has emerged as a promising technology for biosensing applications due to its ability to precisely manipulate small volumes of fluids. Microfluidics platforms combined with spectroscopic techniques are capable of developing miniaturized devices that can detect and quantify pathogenic samples. The review focuses on the advancements in microfluidic devices integrated with spectroscopic methods for detecting bacterial microbes over the past five years. The review is based on several spectroscopic techniques, including fluorescence detection, surface-enhanced Raman scattering, and dynamic light scattering methods coupled with microfluidic platforms. The key detection principles of different approaches were discussed and summarized. Finally, the future possible directions and challenges in microfluidic-based spectroscopy for isolating and detecting pathogens using the latest innovations were also discussed.

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Rapid Quantification of Escherichia coli in Potable Water by Fluorescence In Situ Hybridization Performed in Liquid (liq-FISH) and a Microfluidic System

Cited by 3 publications

References 17 publications

Potential of Flow Cytometric Approaches for Rapid Microbial Detection and Characterization in the Food Industry—A Review

Potential of Flow Cytometric Approaches for Rapid Microbial Detection and Characterization in the Food Industry—A Review

Microfluidics in environmental analysis: advancements, challenges, and future prospects for rapid and efficient monitoring

Recent advances in microfluidic-based spectroscopic approaches for pathogen detection

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