Rapid, quantitative Tube immunoassays for on-site detection of Botrytis, Aspergillus and Penicillium antigens in grape juice

Dewey, F. M.; Meyer, U. M.

doi:10.1016/j.aca.2003.11.088

Cited by 25 publications

(13 citation statements)

References 18 publications

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“…However, immunoassays developed for routine detection of B. cinerea have used monoclonal antibodies that detect other species in the genus Botrytis (Meyer et al. 2000; Dewey and Meyer 2004).…”

Section: Discussionmentioning

confidence: 99%

“…Immunological and nucleic acid‐based methods have been developed for the detection of B. cinerea (Bossi and Dewey 1992; Rigotti et al. 2002; Dewey and Meyer 2004; Suarez et al. 2005; Spotts et al.…”

Section: Introductionmentioning

confidence: 99%

“…Further to the requirement for high sensitivity, some applications also require the ability to detect B. cinerea quantitatively, and this has been achieved using both antibody‐ and nucleic acid‐based methods (Meyer et al. 2000; Dewey and Meyer 2004; Mehli et al. 2005; Suarez et al.…”

Section: Introductionmentioning

confidence: 99%

“…PCR‐based methods generally do not approach the speed and simplicity of field‐portable antibody‐based methods such as lateral flow devices (LFDs) (Lane et al. 2007) or simplified tube‐format ELISA (Dewey and Meyer 2004).…”

Section: Introductionmentioning

confidence: 99%

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Detection of Botrytis cinerea by loop-mediated isothermal amplification

Tomlinson

Dickinson

Boonham

2010

Letters in Applied Microbiology

115

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Aims: To develop a sensitive, rapid and simple method for detection of Botrytis cinerea based on loop‐mediated isothermal amplification (LAMP) that would be suitable for use outside a conventional laboratory setting. Methods and Results: A LAMP assay was designed based on the intergenic spacer of the B. cinerea nuclear ribosomal DNA (rDNA). The resulting assay was characterized in terms of sensitivity and specificity using DNA extracted from cultures. The assay consistently amplified 65 pg B. cinerea DNA. No cross‐reactivity was observed with a range of other fungal pathogens, with the exception of the closely related species Botrytis pelargonii. Use of a novel real‐time LAMP platform (the OptiGene Genie I) allowed detection of B. cinerea in infected rose petals, with amplification occurring in <15 min. Conclusions: The LAMP assay that was developed is suitable for rapid detection of B. cinerea in infected plant material. Significance and Impact of the Study: The LAMP method combines the sensitivity and specificity of nucleic acid‐based methods with simplified equipment and a reduced reaction time. These features make the method potentially suitable for on‐site use, where the results of testing could help to inform decisions regarding the storage and processing of commodities affected by B. cinerea, such as cut flowers, fruit and vegetables.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Detection of Botrytis cinerea by loop-mediated isothermal amplification

Tomlinson

Dickinson

Boonham

2010

Letters in Applied Microbiology

115

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“…Antibodies against water-soluble fungal antigens, in particular against the whole mycelium of B. cinerea have been obtained by some research groups. Polyclonal (Ricker, Marois, Dlott, Bostock, & Morrison, 1991) and monoclonal antibodies (Meyer & Dewey, 2000) were used in plate-trapped antigen-enzyme-linked immunosorbent assays (PTA-ELISAs) or in Tube immunoassays (Dewey & Meyer, 2004) to detect B. cinerea infection in grape juice. However, the use of antibodies is always associated to the risk of cross-reactivity with proteins derived from other fungi, such as Aspergillus and Penicillium, and could therefore be a rather imprecise way to monitor infection due to B. cinerea.…”

Section: Identification Of Protein Spotsmentioning

confidence: 99%

Identification of potential protein markers of noble rot infected grapes

et al. 2015

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Post-harvest proteomics and food security

et al. 2013

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To guarantee sufficient food supply for a growing world population, efforts towards improving crop yield and plant resistance should be complemented with efforts to reduce post-harvest losses. Post-harvest losses are substantial and occur at different stages of the food chain in developed and developing countries. In recent years, a substantially increasing interest can be seen in the application of proteomics to understand post-harvest events. In the near future post-harvest proteomics will be poised to move from fundamental research to aiding the reduction of food losses. Proteomics research can help in reducing food losses through (i) identification and validation of gene products associated to specific quality traits supporting marker-assisted crop improvement programmes, (ii) delivering markers of initial quality that allow optimisation of distribution conditions and prediction of remaining shelf-life for decision support systems and (iii) delivering early detection tools of physiological or pathogen-related post-harvest problems. In this manuscript, recent proteomics studies on post-harvest and stress physiology are reviewed and discussed. Perspectives on future directions of post-harvest proteomics studies aiming to reduce food losses are presented.

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Rapid, quantitative Tube immunoassays for on-site detection of Botrytis, Aspergillus and Penicillium antigens in grape juice

Cited by 25 publications

References 18 publications

Detection of Botrytis cinerea by loop-mediated isothermal amplification

Detection of Botrytis cinerea by loop-mediated isothermal amplification

Identification of potential protein markers of noble rot infected grapes

Post-harvest proteomics and food security

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