Rapid recovery of Mycobacterium tuberculosis complex from clinical specimens using the BACTEC 9000 MB system, a new automated fluorimetric technique

D'Alagni, Marina; Prete, Raffaele Del; Simone, Anna Maria; Santis, Antonio de; Miragliotta, G.

doi:10.1111/j.1469-0691.1997.tb00625.x

Cited by 5 publications

(3 citation statements)

References 13 publications

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“…The recently developed MB/BacT system was one of the first fully automated systems for the culture of mycobacteria approved by the Food and Drug Administration since July 1996. Other automated systems for the culture of mycobacteria have been recently evaluated, including the Difco ESP culture system II (24) and the BACTEC 9000 MB system (13,16).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the MB/BacT system and comparison to the BACTEC 460 system and solid media for isolation of mycobacteria from clinical specimens

et al. 1997

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The MB/BacT automated system is designed for the isolation of mycobacteria from clinical specimens. It utilizes a colorimetric sensor and reflected light to continuously monitor the CO 2 concentration in the culture medium. We compared its performance to that of the BACTEC 12B media for the radiometric BACTEC 460 instrument and that of solid culture media. Respiratory specimens and urine samples were decontaminated with 4% NaOH. The vials of the two instruments were inoculated with 500 l of sample and two solid egg-based media at 200 l each. All vials were incubated at 37°C for 6 weeks. A total of 1,078 specimens (633 respiratory specimens, 78 cerebrospinal fluid specimens, 177 other body fluid specimens, 87 urine specimens, and 103 other types of specimens) were cultured in parallel. Mycobacteria could be identified from 73 (6.8%) specimens: 67 M. tuberculosis, 3 M. kansasii, 1 M. xenopi, 1 M. terrae, and 1 mixed M. avium with M. scrofulaceum. Of these, 63 (86.3%) specimens were positive with the MB/BacT system, 67 (91.8%) were positive with the BACTEC 460 instrument, and 58 (79.5%) were positive with the two egg-based media. MB/BacT cultures were positive on average after 17.5 (؎6.4) days, BACTEC cultures with a growth index of >20 (mean, 200) were positive after 14.3 (؎8.2) days, and egg-based media were positive after 24.2 (؎7.5) days. Microorganisms other than mycobacteria contaminated 46 (4.3%) MB/BacT cultures and 31 (2.9%) BACTEC cultures, which had to be discarded. The MB/BacT system is a well-automated system for the detection of M. tuberculosis in clinical specimens without using radioactive reagents. Further trials are required to determine whether it is suitable for the culture of nontuberculous mycobacteria.

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Section: Discussionmentioning

confidence: 99%

Evaluation of the MB/BacT system and comparison to the BACTEC 460 system and solid media for isolation of mycobacteria from clinical specimens

et al. 1997

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“…Recently, much data has been published on three of these innovative continuous‐monitoring blood‐culture systems, the BacT/Alert, the BACTEC 9000 series, and the ESP (Extra Sensing Power). Interestingly, all three systems have been adapted and evaluated for mycobacterial culture capabilities [79–82].…”

Section: Automated Systemsmentioning

confidence: 99%

Review on evaluations of currently available blood-culture systems

Rohner

Auckenthaler

1999

Clinical Microbiology and Infection

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“…Birçok araştırmada benzer şekilde LJ besiyerinde kontaminasyon oranı, sıvı kültür sistemlerine oranla daha yüksek bulunmuştur. 12,17,18,23,24 Ancak örnek-lerin çeşitliliğine ve kullanılan dekontaminasyon yöntemine bağlı olarak besiyerlerindeki kontaminasyon oranları da farklılık gösterebilir.…”

Section: Bulgularunclassified

Comparison of Löwenstein Jensen Medium and Bactec 460TB Culture System for Diagnosis of Tuberculosis

Başkesen¹,

Sürücüoğlu²,

Özkütük³

et al. 2010

Turkiye Klinikleri J Med Sci

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1469Tüberküloz Tanısında Löwenstein Jensen Besiyeri ile BACTEC 460TB Kültür Sisteminin Karşılaştırılması Ö ÖZ ZE ET T A Am ma aç ç: : Bu çalışmada tüberküloz tanısında BACTEC 460TB sıvı kültür sistemi ile birlikte Löwenstein Jensen besiyeri kullanımının, bakterinin izolasyon oranına katkı sağlayıp sağlamadığı araştırılmıştır. G Ge er re eç ç v ve e Y Yö ön nt te em ml le er r: : Araştırmada toplam 4237 örneğin mikroskobik inceleme ve kültür sonuçları değerlendirilmiştir. Örneklerin 2719'u solunum yolundan, 1518'i ise solunum yolu dışından elde edilen hasta örnekleridir. B Bu ul lg gu ul la ar r: : Kültür sonuçlarına göre, 271 örnekte (%6.4) BACTEC 460TB sisteminde, 238 örnekte (%5.6) ise Löwenstein Jensen besiyerinde Mycobacterium tuberculosis kompleks üremiştir. Kontaminasyon oranları 12B şişelerinde %3.6, Löwenstein Jensen besiyerinde ise %10.2'dir. Üreme süreleri ise BACTEC sisteminde ortalama 9.6 gün, Löwenstein Jensen besiyerinde ise 21 gün olarak belirlenmiştir. Kontamine olan örnekler çıkarıldığında, 3718 örneğin 258'inde (%6.9) örnekte en az bir kültür yöntemi ile Mycobacterium tuberculosis kompleks ürediği gözlenmiştir. BACTEC 460TB kültür sisteminin duyarlılığı %96, Löwenstein Jensen besiyerinin duyarlılığı ise %92 olarak bulunmuştur. Her iki kültür yöntemi arasında %99 oranında uyum gözlenmiştir. Tek başına BACTEC 460TB sisteminin ve LJ besiyerinin kültür pozitifliğine katkısı sırası ile %8.3 ve %3.9 olarak bulunmuştur. Kültürde üreyen örneklerin mikroskobik inceleme sonuçları ile üredikleri kültür sistemleri karşılaştırıldığında istatistiksel olarak anlamlılık gözlenmemiştir. S So on nu uç ç: : Tüberkülozun hızlı tanısında sıvı kültür sistemlerinin önemli bir gereksinim olduğu, ancak en yüksek izolasyon oranına ulaşabilmek için hem sıvı hem de katı kültür sistemlerinin birlikte kullanılmasının gerektiği düşünülmüştür.A An na ah h t ta ar r K Ke e l li i m me e l le er r: : Mycobacterium tuberculosis; kültür teknikleri A AB BS S T TR RA AC CT T O Ob b j je ec c t ti i v ve e: : In this study, it was intestigated that whet her the use of Lö wens te in Jen sen (LJ) me di um with BAC TEC460 TB li qu id cul tu re system con tri bu ted to bac te ri a iso la ti on ra tes for di ag no sis of tu ber cu lo sis. M Ma a t te e r ri i a al l a an nd d M Me et t h ho od ds s: : A to tal of 4237 spe ci mens we re eva lu a ted in the study for the re sults of mic ros co pic exa mi na ti on and cul tu re. Of 4237 spe ci mens, 2719 we re ob tai ned from res pi ra tory tract and re ma i ning 1518 we re ob ta i ned out of the res pi ra tory tract. R Re e s su ul lt ts s: : Ac cor ding to the re sults of cul tu re, Myco bac te ri um tu ber cu lo sis comp lex we re iso la ted in 271 (6.4%) spe ci mens on BAC TEC 460TB system and in 238 (5.6%) spe ci mens on Lö wens te in Jen sen me di um. Con ta mi na ti on ra tes at BAC TEC 460TB system and Lö wens te in Jen sen me di um we re 3.6% and 10.2%, res pec ti vely. The me an ti mes of re co very we re de ter mi ned as 9.6 days for BAC -TEC system and 21 days for Lö wens te in ...

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Rapid recovery of Mycobacterium tuberculosis complex from clinical specimens using the BACTEC 9000 MB system, a new automated fluorimetric technique

Cited by 5 publications

References 13 publications

Evaluation of the MB/BacT system and comparison to the BACTEC 460 system and solid media for isolation of mycobacteria from clinical specimens

Evaluation of the MB/BacT system and comparison to the BACTEC 460 system and solid media for isolation of mycobacteria from clinical specimens

Review on evaluations of currently available blood-culture systems

Comparison of Löwenstein Jensen Medium and Bactec 460TB Culture System for Diagnosis of Tuberculosis

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