Rapid Removal of Matrices from Small‐Volume Samples by Step‐Voltage Nanoelectrospray

Wei, Zhenwei; Han, S.; Gong, Xiaoyun; Zhao, Yaoyao; Yang, Chengdui; Zhang, Sichun; Zhang, Xinrong

doi:10.1002/anie.201302870

Cited by 56 publications

(54 citation statements)

References 40 publications

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“…Typical mass spectra (Figure e) of zone 1 are very noisy; the spectrum of zone 2 is very clean with the analyte peptides displaying very high SNR and enhanced signal intensity, while the spectrum of zone 3 shows matrix peaks. These results are consistent with the following proposed mechanism, based on our prior study of this effect: during electrophoresis (−5 kV voltage applied to the electrode) a strong static electrical field in the solution pulls small cations and positively charged complexes into zone 3 (they show up later as the interference peaks in the MS of zone 3); the initial negative potential also pushes small anions into zone 1 so cleaning the analyte in zone 2 of interfering negatively charged ions. By removal of the high mobility ions from zone 2, a commensurate narrowing of the bandwidth and preconcentration of weak electrolytes (e.g.…”

Section: Figuresupporting

confidence: 90%

High‐Throughput Bioassays using “Dip‐and‐Go” Multiplexed Electrospray Mass Spectrometry

et al. 2019

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A multiplexed system based on inductive nanoelectrospray mass spectrometry (nESI‐MS) has been developed for high‐throughput screening (HTS) bioassays. This system combines inductive nESI and field amplification micro‐electrophoresis to achieve a “dip‐and‐go” sample loading and purification strategy that enables nESI‐MS based HTS assays in 96‐well microtiter plates. The combination of inductive nESI and micro‐electrophoresis makes it possible to perform efficient in situ separations and clean‐up of biological samples. The sensitivity of the system is such that quantitative analysis of peptides from 1–10 000 nm can be performed in a biological matrix. A prototype of the automation system has been developed to handle 12 samples (one row of a microtiter plate) at a time. The sample loading and electrophoretic clean‐up of biosamples can be done in parallel within 20 s followed by MS analysis at a rate of 1.3 to 3.5 s per sample. The system was used successfully for the quantitative analysis of BACE1‐catalyzed peptide hydrolysis, a prototypical HTS assay of relevance to drug discovery. IC50 values for this system were in agreement with LC‐MS but recorded in times more than an order of magnitude shorter.

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Section: Figuresupporting

confidence: 90%

High‐Throughput Bioassays using “Dip‐and‐Go” Multiplexed Electrospray Mass Spectrometry

et al. 2019

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“…[1][2][3][4] The multi-stage pretreatment procedures are usually laborious, timeconsuming, and expensive in operation and commonly suffer from sample losses and chemical contamination. Significant efforts are being made to develop more practical and versatile approaches for chemical preconcentration and purification relying on the recent advances in adsorbent materials, [5][6][7] microfluidics, 8 nanotechnology, 9 micro-extraction, 10 crystallization, 11 electrospray ionization (ESI) [12][13][14] and matrix-assisted laser desorption/ionization (M ALDI). 15 -17 It has been earlier discovered that the concentration of organic solutes in bursting bubble aerosols above the sea surface is increased by more than two orders of magnitude relative to the bulk seawater whereas the chemical enrichment of inorganic constituents (including Na + , M g 2+ , Ca 2+ , K + , Cl -, and SO 4 2-) is limited to a few percent if occurs at all.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Preconcentration and Desalting of Organic Solutes in Aqueous Solutions by Bubble Bursting

et al. 2016

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Significant efforts are being made to develop more practical and versatile approaches for the preconcentration and purification of complex chemical samples. Inspired by the naturally occurring enrichment of organic compounds in sea aerosols, in this study we demonstrate the potential of induced bubble bursting as an approach for the preconcentration of organic solutes in various aqueous solutions. Apart from the preconcentration of organics, notable decrease in the concentration of metal salt components was discovered for the first time. On the basis of a series of model experiments, the phenomenon has been attributed to intermolecular competition at the surface interface of rising bubbles. Overall, our results indicate the high versatility and simplicity of the bubble bursting approach for the simultaneous preconcentration and desalting of organic solutes in aqueous solutions for mass spectrometry, chromatography, optical detection, and other fields of analysis.

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“…2). Besides, our method could avoid the matrix effects caused by the complexity of the sample33, even if sample purification was not conducted, because 1) some matrix, such as salts, could not dissolve into pure methanol, resulting in some tolerance of matrix effects; 2) single reaction monitoring (SRM) was always utilized to identify Cyt and 5mCyt in MS 2 spectra. Therefore, SRM were always used to identify Cyt and 5mCyt while the samples were ionized.…”

Section: Resultsmentioning

confidence: 99%

DNA methylation as a potential diagnosis indicator for rapid discrimination of rare cancer cells and normal cells

Zhao

Yang

et al. 2015

Sci Rep

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The global DNA methylation degree may be a ubiquitous and early biomarker to distinguish cancer cells from benign cells. However, its usefulness in clinical diagnosis was scarcely demonstrated, because the cancer cells isolated from patients were usually very rare. Even if 10 mL of peripheral blood was sampled from a patient, only tens of cancer cells could be isolated. So a method to quantify DNA methylation from small number of cells was needed to apply DNA methylation in clinical environment. In this study, we found that normal breast cell line MCF10A and breast cancer cell line MCF7 cells present significantly different percentage of genomic 5-methylcytosine (p < 0.02, n = 8), it could be a potential indicator for rapid discrimination of rare cancer cells from normal cells. However, conventional mass spectrometry needs usually ~106 cells to quantify DNA methylation degree, which was too large to be applied in clinical diagnosis. Here we developed a fast mass spectrometry-based method capable of analyzing the DNA methylation degree from only ~100 human cells. Our method could reveal the different DNA methylation degree between MCF10A and MCF7 cells in less than two hours, having the potential to provide reliable information for clinical application.

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Rapid Removal of Matrices from Small‐Volume Samples by Step‐Voltage Nanoelectrospray

Cited by 56 publications

References 40 publications

High‐Throughput Bioassays using “Dip‐and‐Go” Multiplexed Electrospray Mass Spectrometry

High‐Throughput Bioassays using “Dip‐and‐Go” Multiplexed Electrospray Mass Spectrometry

Simultaneous Preconcentration and Desalting of Organic Solutes in Aqueous Solutions by Bubble Bursting

DNA methylation as a potential diagnosis indicator for rapid discrimination of rare cancer cells and normal cells

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