Rapid screening and separation of active compounds against α-amylase from Toona sinensis by ligand fishing and high-speed counter-current chromatography

Meng, Jing; Li, Qingyue; Cao, Zengyuan; Gu, Dongyu; Wang, Yunxiao; Zhang, Yunci; Wang, Yi; Yang, Yi; He, Fei

doi:10.1016/j.ijbiomac.2021.01.195

Cited by 34 publications

(8 citation statements)

References 42 publications

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“…Owing to its high sample recovery and repeatability, HSCCC has become the preferred separation method for the separation of target compounds from complex natural extracts (Meng et al, 2021). In HSCCC separation, the most important step is the selection of the two‐phase solvent system, with an important criterion that the K‐ value should be in the range 0.4–2.5 (Friesen & Pauli, 2005).…”

Section: Resultsmentioning

confidence: 99%

“…Dried Fe 3 O 4 (10 mg) and dopamine solution (8 mg/ml, 1 ml) were dispersed into Tris–HCl buffer (1 ml, pH 8.5) in the centrifugal tube and reacted at 1000 rpm for 10 h to obtain the polydopamine‐wrapped Fe 3 O 4 @PDA (Meng et al, 2021). Fe 3 O 4 @PDA was mixed with glutaraldehyde (3%) in the centrifugal tube at 1,000 rpm for 1 h. After washing with PBS buffer solution (pH 5.7) and removing the supernatant, 1 ml of α ‐amylase solution (1 mg/ml) and 1 ml of α ‐glucosidase solution (5 μl/ml) were added to the crosslinked Fe 3 O 4 @PDA and mixed at 1,000 rpm for 4 h. The magnetic bienzymes were co‐immobilized by the covalent method.…”

Section: Methodsmentioning

confidence: 99%

“…tube and reacted at 1000 rpm for 10 h to obtain the polydopamine-wrapped Fe 3 O 4 @PDA (Meng et al, 2021).…”

Section: Co-immobilization Of Bienzymesmentioning

confidence: 99%

Section: Isolation and Identification Of The Target Compoundmentioning

confidence: 99%

“…Common advanced materials, such as hollow fibers (Yu et al, 2014), nanotubes (Wang et al, 2022) and metal–organic frameworks (Zhong et al, 2019), can be used to immobilize enzymes for improving the stability and reusability of the enzymes (Guo et al, 2023). In addition, in order to facilitate the recovery of immobilized enzymes, the introduction of Fe 3 O 4 with good dispersion and magnetism is a very good solution (Meng et al, 2021). Active compounds can be affinity screened from complex mixtures like fishing with immobilized enzymes under the action of an external magnetic field.…”

Section: Introductionmentioning

confidence: 99%

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Dual‐target affinity analysis and separation of α‐amylase and α‐glucosidase inhibitors from Morus alba leaves using a magnetic bifunctional immobilized enzyme system

Guo

Liu

Meng

et al. 2022

Biomedical Chromatography

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Morus alba leaves are a natural product with great antidiabetic potential. However, the therapeutic efficacy of natural products is usually achieved through the interaction of active compounds with specific targets. Among them, active compounds with multi‐target therapeutic functions are more effective than single‐target enzymes. In this study, a bienzyme system was constructed by co‐immobilizing α‐amylase and α‐glucosidase onto Fe3O4 for affinity screening of dual‐target active components in the complex extract from M. alba leaves. As a result, a potential active compound was selectively screened by ligand fishing, separated by high‐speed countercurrent chromatography using a solvent system of ethyl acetate–n‐butanol–water (3:2:5, v/v), and identified as rutin. In addition, the result of molecular docking showed that rutin could interact with the active center of α‐amylase and α‐glucosidase through multiple hydrogen bonds, van der Waals forces, etc. to play an inhibitory role. These results demonstrate the effectiveness of the polydopamine magnetically immobilized bienzyme system for dual‐target affinity screening of active substances. This study not only reveals the chemical basis of the antidiabetic activity of M. alba leaves from a dual‐target perspective, but also promotes the progress of multitarget affinity screening.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…tube and reacted at 1000 rpm for 10 h to obtain the polydopamine-wrapped Fe 3 O 4 @PDA (Meng et al, 2021).…”

Section: Co-immobilization Of Bienzymesmentioning

confidence: 99%

Section: Isolation and Identification Of The Target Compoundmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Dual‐target affinity analysis and separation of α‐amylase and α‐glucosidase inhibitors from Morus alba leaves using a magnetic bifunctional immobilized enzyme system

Guo

Liu

Meng

et al. 2022

Biomedical Chromatography

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Ellagic acid from pomegranate peel: Consecutive countercurrent chromatographic separation and antioxidant effect

Yang

Liu

et al. 2023

Biomedical Chromatography

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Ellagic acid is one of the most representative natural antioxidants, and is rich in pomegranate peel. In this study, a consecutive countercurrent chromatographic (CCC) separation method was established to improve the preparative efficiency of ellagic acid from pomegranate peel. By optimizing the solvent system, sample size and flow rate, 280 mg of ellagic acid was obtained from 5 g of crude sample from pomegranate peel by CCC after six consecutive injections. Moreover, the values of EC50 for ellagic acid in scavenging ABTS·+ and DPPH· were 4.59 ± 0.07 and 10.54 ± 0.07 μg/ml, respectively, indicating a strong antioxidant activity. This study not only established a high‐throughput method for the preparation of ellagic acid, but also provided a successful example for the development of and research on other natural antioxidants.

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Recent Advances in Screening and Separating Active Components From Natural Products Based on High‐Speed Countercurrent Chromatography

Wang,

Huang,

Zhang

et al. 2024

J of Separation Science

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Natural products play a vital role in human health because people find that they can reduce the incidence of many diseases, such as cancer and cardiovascular diseases. Therefore, researchers have developed many effective techniques for screening and separating active compounds from natural products. High‐speed countercurrent chromatography is a unique unsupported liquid–liquid chromatography technology that has been widely used in the separation of natural products. In this paper, the research progress of screening and separating active components from natural products by combining high‐speed countercurrent chromatography technology with other technologies is reviewed from two aspects: pre‐column activity assay and post‐column activity assay. The purpose of this paper is to provide some theoretical support for the screening and separation of natural active compounds by high‐speed countercurrent chromatography. I hope that the contents summarized in this paper can provide more basis for the development of screening and separation of natural active compounds to realize large‐scale preparation.

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Rapid screening and separation of active compounds against α-amylase from Toona sinensis by ligand fishing and high-speed counter-current chromatography

Cited by 34 publications

References 42 publications

Dual‐target affinity analysis and separation of α‐amylase and α‐glucosidase inhibitors from Morus alba leaves using a magnetic bifunctional immobilized enzyme system

Dual‐target affinity analysis and separation of α‐amylase and α‐glucosidase inhibitors from Morus alba leaves using a magnetic bifunctional immobilized enzyme system

Ellagic acid from pomegranate peel: Consecutive countercurrent chromatographic separation and antioxidant effect

Recent Advances in Screening and Separating Active Components From Natural Products Based on High‐Speed Countercurrent Chromatography

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