Rapid, sensitive and versatile determination of selenium in different biological samples

Giguère, A.; Fortier, Marie-Ève; Matte, J. J.

doi:10.4141/a05-044

Cited by 10 publications

(4 citation statements)

References 9 publications

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“…a The calculated compositions for ME, CP, lysine, Ca, and P of the basal diet were 2702 kcal/kg, 14.0, 0.6, 1.0, and 0.6% respectively. b The basal Se and pyridoxine contents of the diet were 0.3 and 1.7 mg/kg respectively (analytical values determined according to Matte et al (2001) and Giguère et al (2005) respectively).…”

Section: Samplingmentioning

confidence: 99%

“…Measurements of Se were done using a fluorimetric method adapted by Giguère et al (2005) from the technique of Sheehan & Gao (1990) and P-5-P was determined using a fluorimetric method adapted by Matte et al (1997) from the technique of Srivastava & Beutler (1973). SeGPX activity was determined in blood plasma by the spectrophotometric method described by Gunzler & Flohe (1985).…”

Section: Laboratory Analysismentioning

confidence: 99%

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Gene expression of porcine blastocysts from gilts fed organic or inorganic selenium and pyridoxine

Dalto¹,

Tsoi²,

Audet³

et al. 2015

Reproduction

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In this study, we determined how maternal dietary supplementation with pyridoxine combined with different sources of selenium (Se) affected global gene expression of porcine expanded blastocysts (PEB) during pregnancy. Eighteen gilts were randomly assigned to one of the three experimental diets (nZ6 per treatment): i) basal diet without supplemental Se or pyridoxine (CONT); ii) CONTC0.3 mg/kg of Na-selenite and 10 mg/kg of HCl-pyridoxine (MSeB 6 10); and iii) CONTC0.3 mg/kg of Se-enriched yeast and 10 mg/kg of HCl-pyridoxine (OSeB 6 10). All gilts were inseminated at their fifth post-pubertal estrus and killed 5 days later for embryo harvesting. A porcine embryospecific microarray was used to detect differentially gene expression between MSeB 6 10 vs CONT, OSeB 6 10 vs CONT, and OSeB 6 10 vs MSeB 6 10. CONT gilts had lower whole blood Se and erythrocyte pyridoxal-5-P concentrations than supplemented gilts (P!0.05). No treatment effect was observed on blood plasma Se-glutathione peroxidase activity (PZ0.57). There were 10, 247, and 96 differentially expressed genes for MSeB 6 10 vs CONT, OSeB 6 10 vs CONT, and OSeB 6 10 vs MSeB 6 10 respectively. No specific biological process was associated with MSeB 6 10 vs CONT. However, for OSeB 6 10 vs CONT, upregulated genes were related with global protein synthesis but not to selenoproteins. The stimulation of some genes related with monooxygenase and thioredoxin families was confirmed by quantitative real-time RT-PCR. In conclusion, OSeB 6 10 affects PEB metabolism more markedly than MSeB 6 10. Neither Se sources with pyridoxine influenced the Se-glutathione peroxidase metabolic pathway in the PEB, but OSeB 6 10 selectively stimulated genes involved with antioxidant defense.

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Section: Samplingmentioning

confidence: 99%

Section: Laboratory Analysismentioning

confidence: 99%

Gene expression of porcine blastocysts from gilts fed organic or inorganic selenium and pyridoxine

Dalto¹,

Tsoi²,

Audet³

et al. 2015

Reproduction

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“…Whole blood Se was chosen in the present experiment as an indicator of circulating Se. It possibly reflects better the animal Se status, because more than 50% of the overall Se in circulation is presented in erythrocytes, mainly bound to hemoglobin (19). The studies in poultry (20) and goats (21), failed to show any effect of Se treatment on the activity of blood GPx.…”

Section: Discussionmentioning

confidence: 99%

Improvement of the Antioxidative Status of Pig Ovaries by Selenopyran Treatment

Abadjieva

Kistanova

Marchev³

et al. 2014

Macedonian Veterinary Review

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Original Scientific ArticleSelenium is an essential micro-element in animal diet due to its high antioxidative properties. As a part of selenocystein it is an important constituent of the glutathione peroxidase (GPx) enzyme, which has a big importance for cell protection from oxidative damage. The aim of the present work was the investigation of the selenopyran effect on the antioxidative state of the pig ovary. The experiment was conducted with 18 gilts of Danube white breed randomly divided into two groups between 120 -228 days of age. The animals received equal basal diets without selenium additives. The experimental gilts were injected once per month intramuscularly with oil solution of preparation selenopyran (9-phenylsymmetrical octahydroselenoxanthene) ensured 0.1 mgSe/kg live weight. After slaughtering, the ovaries were used for histological analysis and estimation of the selenium content in ovarian tissue by the atomic absorption spectroscopy method. The GPx activity in ovary homogenates using the colorimetric assay kit (BioVision) was measured. The expression of γ-glutamyl transpeptidase (GGT) in ovaries by immunochistochemical method was estimated. The selenopyran treatment leads to significant (P<0.05) increase of the selenium level in blood and non-significant (P>0.05) in ovarian tissue. Enhancement of GPx activity in the ovaries of experimental group was observed (142.61±6.6 versus 122.28±3.4U/gP, P<0.05). The GGT expression in the ovarian cortex cells, follicular fl uid and in the erythrocytes of ovarian blood vessels in treated gilts was an evidence of active transport of glutathione from blood to the ovary tissue. The selenopyran treatment promotes the increase of the GPx dependent antioxidative defense in ovary of growing gilts.

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“… The basal Se and pyridoxine content of the diet was 0.2 and 1.7 mg/kg, respectively (Dalto et al., ,b), and 0.3 and 2.4 mg/kg, respectively (Dalto et al., ) (analytical values determined according to Giguère et al. () and Matte et al. () respectively). …”

Section: Methodsmentioning

confidence: 99%

Assessment of antioxidative and selenium status by seleno‐dependent glutathione peroxidase activity in different blood fractions using a pig model: issues for clinical nutrition and research

Dalto

Lapointe

Matte

2017

Animal Physiology Nutrition

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SummaryBlood seleno-dependent glutathione peroxidase (SeGPX) activity is widely used as a metabolic indicator of systemic antioxidative status despite inconsistent responses in the literature. This study aimed to compare SeGPX activity profiles in different blood fractions, expressed with different reference units, and assess their impact on interpretation of results. Two studies on selenium (Se) metabolism in gilts, including long-term and peri-oestrus SeGPX activity profiles, were submitted to analysis of variance with double repeated measures, after data set standardization. Differences between studies were experimental period (three post-pubertal oestrus or five postpubertal oestrus +30 days of gestation) and sample type (whole blood or blood plasma). No difference was observed between whole-blood long-term profiles (three oestrus) for SeGPX activity/mg haemoglobin (SeGPXhb) vs. SeGPX activity/ml whole blood (SeGPXwb; p = 0.29). No long-term difference was observed in whole blood between profiles according to dietary Se provision (basal and dietary Se-supplemented groups; p ≥ 0.12). Blood plasma long-term profiles (five oestrus + 30 days gestation) for SeGPX/mg blood plasma protein (SeGPXpro) were different from SeGPX/ml blood plasma (SeGPXpla) according or not to Se provision (p ≤ 0.007 and p < 0.001 respectively). However, regardless of Se provision (p ≥ 0.80), when excluding gestation from the model, blood plasma profiles were similar. During the peri-oestrus period (day À4 to +3), regardless of Se provision, SeGPX activity profiles differed according to reference units in both studies (p < 0.001). However, considering Se provision, similar profiles were observed in whole blood and blood plasma (p ≥ 0.27) for basal Se groups, whereas in Se-supplemented groups they differed for both sample types (p ≤ 0.02). In conclusion, reference units influence interpretation of SeGPX activity according to physiological state. During oxidative stress periods, this effect depends upon dietary Se provision.

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Rapid, sensitive and versatile determination of selenium in different biological samples

Cited by 10 publications

References 9 publications

Gene expression of porcine blastocysts from gilts fed organic or inorganic selenium and pyridoxine

Gene expression of porcine blastocysts from gilts fed organic or inorganic selenium and pyridoxine

Improvement of the Antioxidative Status of Pig Ovaries by Selenopyran Treatment

Assessment of antioxidative and selenium status by seleno‐dependent glutathione peroxidase activity in different blood fractions using a pig model: issues for clinical nutrition and research

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