Rapid, Sensitive Colorimetric Method for Determination of Ethinyl Estradiol

Eldawy, M. A.; Tawfik, A.S.; El-Shabouri, Salwa R

doi:10.1002/jps.2600640722

Cited by 16 publications

(7 citation statements)

References 12 publications

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“…However, determination of ETE directly in contraceptive formulations was not possible because of the interference of other compounds in the UV spectral region, as previously observed by Berzas-Nevada et al 2 In a previous work, a colorimetric procedure, based on the formation of an azo dye by the condensation of diazotized 5-chloro-2,4-dinitroaniline with ethinylestradiol, was described. 20 In this work, three nitroanilines (2-methoxy-4nitroaniline, 2,4-dinitroaniline and 2-methyl-4-nitroaniline), available in our laboratory, were tested as spectrophotometric reagents for determination of ethinylestradiol (ETE). The determinations were based on absorbance measurments of the reaction product obtained from the coupling of each diazotized nitroaniline with ETE.…”

Section: Resultsmentioning

confidence: 99%

“…19 Formation of products with stable colours by diazotized nitroanilines coupled with phenolic compounds is the basis for a rapid and sensitive method for determination of pharmaceuticals containing a phenolic hydroxyl group such as ETE. 20 In this work, three nitroanilines (2-methoxy-4-nitroaniline, 2,4-dinitroaniline and 2-methyl-4-nitroaniline) were tested as spectrophotometric reagents for determination of ethinylestradiol (ETE) and a procedure for determination of ETE in pharmaceutical formulations is proposed. The method is based on absorbance measurments of the reaction product of the coupling reaction between diazotized 2-methyl-4-nitroaniline and ETE, which is a convenient and stable compound for spectrophotometric determination.…”

Section: Introductionmentioning

confidence: 99%

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Use of nitroanilines for spectrophotometric determination of ethinylestradiol in pharmaceutical formulations

Teixeira

Bueno

et al. 2011

Anal. Methods

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Three nitroanilines (2-methoxy-4-nitroaniline, 2,4-dinitroaniline and 2-methyl-4-nitroaniline) were tested as spectrophotometric reagents for determination of ethinylestradiol (ETE). The determinations were based on absorbance measurments of the reaction product obtained from the coupling of each diazotized nitroaniline with ETE. Optimization of temperature, pH and diazotization coupling reaction time was realized. 2-Methyl-4-nitroaniline provided the more sensitive system for ETE spectrophotometric determination at 531 nm and the analytical response was linear in the concentration range of 0.65-10.0 mg ml À1 . The limit of detection and coefficient of variation were estimated as 197 mg l À1 and 4.5%, respectively. The results showed that the proposed method is simple and rapid, allowing selective determination of analyte. The method successfully determined ETE in oral contraceptive formulations.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Use of nitroanilines for spectrophotometric determination of ethinylestradiol in pharmaceutical formulations

Teixeira

Bueno

et al. 2011

Anal. Methods

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“…The structure of gestodene (GTD) has a characteristic A4-3-keto group GTD cn~ I OH C~CH CH i..-in the A-ring with a different chromophoric power to ethinylestradiol. Several studies has been reported 1-12 for the determination of contraceptive agents including the use of radio-inmunoassay [1], radioactively labelled derivatives [2,3], dansyl or other fluorescent derivatives [4][5][6], spectrophotometry [7][8][9] and high-performance liquid chromatography (HPLC) [10][11][12]. No references were found for ETE and GTD in capillary electrophoresis.…”

Section: Ete Ohmentioning

confidence: 99%

Determination of ethinylestradiol and gestodene in oral contraceptives by micellar electrokinetic chromatography

et al. 1999

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SummaryA micellar electrokinetic chromatographic method is presented which permits quantification of ethinylestradiol and gestodene in pharmaceutical products. Separation was carried out at 25~ and 25 KV, using a 20 mM borate buffer (pH 9.2), 15 mM sodium dodecylsulfate in 30% acetonitrile-water (v/v). Under these conditions analyses were carried out in 7 min. Four different oral contraceptives were analysed and the results compared favourably with those of a reference liquid chromatographic method.

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“…Literature survey reveals that several methods were reported for estimation of like HPLC [3][4][5][6][7][8][9][10][11], RPHPLC [12,13]. Derivative spectrophotmetry [14], LC [15], HPLC-MS [16,17] Colorimetry [18].…”

Section: Introductionmentioning

confidence: 99%

Method Development and Validation for Desogestrel and Ethinylestradiol in Combined Pharmaceutical Dosage Form by RP-HPLC

Chandewar¹

2013

Pharm Anal Acta

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A simple, rapid, sensitive RP-HPLC method for the simultaneous determination of Desogestrel and Ethinyloestradiol in pharmaceutical dosage forms was developed the analyte were resolved using KH 2 PO 4 Buffer (0.02M): Acetonitrile (50:50), at a flow rate of 2.0ml/min, on HPLC auto sampler system containing UV-visible and fluorescence detector with Empower software and Zorbax SB Phenyl C18 column (4.6×150 mm). Detector Fluorescence detector for Ethinylestradiol UV detector for Desogestrel, For the estimation the detection wavelength was taken as 310 nm Emission and 285 nm excitation Ethinyloestradiol and 210 nm for Desogestrel. Linearity for detector response was observed in the concentration range of 10-150% of test concentration. Correlation coefficient (r) for calibration curve was found to be 1.0. Retention times were found to be 2.4 min and 13.9 min for Ethinyloestradiol and Desogestrel respectively. Percent recovery was found to be within the range of 98.0% to 102.0%. The percent RSD for the analyzed tablet and recovery studied was less than 2. The results of recovery studies were found to be linear in the range 50% to 150% of test concentration. Results of the analysis were validated statistically and by recovery studies. The developed method was found to be precise, selective and rapid for the simultaneous determination of Desogestrel and Ethinyloestradiol in bulk and in pharmaceutical dosage form.

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Rapid, Sensitive Colorimetric Method for Determination of Ethinyl Estradiol

Cited by 16 publications

References 12 publications

Use of nitroanilines for spectrophotometric determination of ethinylestradiol in pharmaceutical formulations

Use of nitroanilines for spectrophotometric determination of ethinylestradiol in pharmaceutical formulations

Determination of ethinylestradiol and gestodene in oral contraceptives by micellar electrokinetic chromatography

Method Development and Validation for Desogestrel and Ethinylestradiol in Combined Pharmaceutical Dosage Form by RP-HPLC

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