2016
DOI: 10.4236/jasmi.2016.62004
|View full text |Cite
|
Sign up to set email alerts
|

Rapid Separation of Five Cyclosporin Analogs by Supercritical Fluid Chromatography

Abstract: Recently there has been a resurgence of interest in cyclic peptides due to their therapeutic advantages in terms of potency, permeability, proteolytic stability, and unique selectivity relative to traditional smaller drug molecules. Cyclosporin is a family of cyclic peptides widely used as autoimmune suppression agents. Cyclosporin analogs consist of eleven amino acids with the main difference lying at the side chain of its amino acid residues. In this study, a single step separation method was developed utili… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

0
5
0

Year Published

2019
2019
2024
2024

Publication Types

Select...
6

Relationship

0
6

Authors

Journals

citations
Cited by 6 publications
(5 citation statements)
references
References 25 publications
0
5
0
Order By: Relevance
“…SFC can be used for the purification of up to 40-mer peptides, both linear and cyclic. It uses CO 2 as a mobile phase with modifiers (e.g., methanol, ethanol, 2-propanol) and additives (e.g., TFA or ammonium acetate). , Zheng et al claimed that the acidity of the mobile phase modifier was critical and the amount of TFA additive varied with different polypeptides . SFC appears to be a promising technique in relation to greener chromatography compared with HPLC, due to the possibility of recycling the CO 2 used during SFC purifications (as yet, still an unresolved topic).…”
Section: Sustainability In Peptide Isolation and Purificationmentioning
confidence: 99%
“…SFC can be used for the purification of up to 40-mer peptides, both linear and cyclic. It uses CO 2 as a mobile phase with modifiers (e.g., methanol, ethanol, 2-propanol) and additives (e.g., TFA or ammonium acetate). , Zheng et al claimed that the acidity of the mobile phase modifier was critical and the amount of TFA additive varied with different polypeptides . SFC appears to be a promising technique in relation to greener chromatography compared with HPLC, due to the possibility of recycling the CO 2 used during SFC purifications (as yet, still an unresolved topic).…”
Section: Sustainability In Peptide Isolation and Purificationmentioning
confidence: 99%
“…SFC has been extensively employed in the past for the purification of chiral compounds, including chiral APIs in the pharmaceutical industry, 253,255 but lately it has been used also in the field of peptides’ separations for analytical purposes, as proved by a rich literature. 256 Stationary phases commonly employed are amine or cyanopropyl-bonded silica or copolymer of styrene and divinylbenzene. 250 a…”
Section: Peptide Purification Improvementsmentioning
confidence: 99%
“…Various working groups reported the use of SFC for the analysis of peptides. Cyclosporine [6], gramicidin [7][8][9], mixtures of chemical diverse peptides [10][11][12][13], or isomeric peptide pairs [14,15] were used to demonstrate its potential. Even longer chain peptides up to 40 amino acids [9,12] or larger glycoproteins up to 80 kDa were separated successfully [16,17].…”
Section: Introductionmentioning
confidence: 99%