Rapid separation of four main taxoids in Taxus species by a combined LLP‐SPE‐HPLC (PAD) procedure

Abstract

A method is described for the simultaneous determination of paclitaxel and three related taxoids, 10-deacetylbaccatin III (10-DAB III), baccatin III, and cephalomannine, in the extracts from the needles of three Chinese yew species, Taxus cuspidata, T. chinensis, and T. media. SPE was applied as the sample preparation technique and RP-HPLC with a photodiode array detector (PAD) was used for the analysis of extract samples. The crude extracts were treated with an improved SPE cartridge packed with a combination of 1-vinyl-pyrrolidin-2-one and divinyl-benzene. The eluent was 75% methanol. The following separation was achieved with a gradient program on an HIQ SIL C18W column in a system of ACN/water within 60 min. The samples were detected by PAD at wavelengths of 232.1 nm for 10-DAB III, 229.8 nm for baccatin III and paclitaxel, and 223.9 nm for cephalomannine. The content of 10-DAB III, baccatin III, cephalomannine, and paclitaxel varied from 0.0277 to 0.0875, 0.0254 to 0.0405, 0.0715 to 0.2486, and 0.0996 to 0.1301 mg/g in fresh needles of the above yew species, respectively. The assay achieved good resolution in the separation between the four compounds, and it can be used for quality control or purity determination for those in bulk and pharmceutical dosage forms.

Simultaneous determination of main taxoids in Taxus needles extracts by solid‐phase extraction‐high‐performance liquid chromatography with pentafluorophenyl column

“…However, the contents of other taxoids such as 10-DAB III and 7-xyl-10-DAT are much higher than paclitaxel in Taxus species, and pharmacological experiments have indicated that some of them, such as 7-xyl-10-DAT, exert strong anti-tumor activity (Lataste et al, 1984). These taxoids are precursors necessary for the biosynthesis of paclitaxel in the plants (Madhusudanan et al, 2002b;Zu et al, 2006;Ballero et al, 2003;Mroczek and Glowniak, 2001). Therefore, these taxoids have become one of the hot spots in the field of paclitaxel research in recent years.…”

“…Solid-phase extraction (SPE) made the determination and preparation stage faster, easier, and more automatable (Son et al, 2000). There are many reports on SPE of taxoids on C 18 sorbent (Mattina and MacEachen, 1994;Glowniak et al, 1996), cyano bonded silica phases (Sparreboom et al, 1995;Huizing et al, 1995), and 1-vinyl-pyrrolidin-2-one and divinyl-benzene (Waters Oasis HLB) sorbent (Zu et al, 2006). Octadecyl-bonded SPE was reported to obtain five taxanes in needle extracts using a PFP column (Mattina and MacEachen, 1994).…”

Determination of paclitaxel and other six taxoids in Taxus species by high-performance liquid chromatography–tandem mass spectrometry

“…It is regarded as one of the most useful anticancer drugs of plant origin available today. It is effective in the treatment of many tumor types, such as ovarian, breast, melanoma, head, neck, bladder, gastrointestinal, lung cancer, and non-small-cell lung cancer [1][2][3], as well as for AIDS-related Kaposi's sarcoma [4][5][6]. Its fundamental anticancer mechanism is unique [7].…”

Preparation and characterization of molecular imprinted polymer functionalized with core/shell magnetic particles (Fe 3 O 4 @SiO 2 @MIP) for the simultaneous recognition and enrichment of four taxoids in Taxus × media

“…However, no superparamagnetic MIP was reported. In this 3 work, the molecularly imprinted particles with a superparamagnetic Fe 3 O 4 @SiO 2 core (Fe 3 O 4 @SiO 2 @MIP) for rapid, selective, simultaneous enrichment and separation of four taxoids in the extracts of T. × Media were synthesized by a multi-step procedure illustrated in Fig. 2 [19].…”

“…Besides paclitaxel numerous related taxoids have been identified in the twigs of T. × media, including 10-deacetylbaccatin III (10-DAB), baccatin III, and cephalomannine ( Fig. 1) [2,3]. Cephalomannine also has important bioactivity [4].…”